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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 285 (1980), S. 169-171 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Physarum actin and myosin were purified as reported previously3. Actomyosin (molar ratio 1:1) was reconstituted by mixing actin and myosin in 20 mM imidazole buffer of pH 7.0, 30 mM KC1, 0.1 mM EDTA and 0.2 mM dithiothreitol (DTT). After centrifugation at 30,000g for 15 min, the precipitated ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 46 (1956), S. 423-436 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. When one end of an internodial cell ofNitella is brought in contact with water, and the other end with the solution of sucrose, transcellular osmosis takes place from water side to the sucrose side. 2. Accompanying transcellular osmosis, the cell sap on the water side is diluted and that on the sucrose side is concentrated. The magnitude of the polar change in sap concentration is dependent on the difference in osmotic pressure between the two external solutions. 3. By tying off an internodial cell ofNitella with strips of silk thread after inducing transcellular osmosis, it is possible to produce cells having arbitrary osmotic pressures within the range of as high as 3 times and as low as 1/4 the normal level. 4. The cells thus produced, which are in fact fragments of the mother internodial cell, can survive indefinitely. 5. The cell, whose osmotic pressure is abnormally high or low, has a marked tendency to be restored to its normal level. 6. The tendency to be restored to the normal osmotic pressure is maintained, if not fully, even when the import or export of solutes to/from the cell is prevented. 7. Turgor pressure of the cell plays no essential role in the osmoregulation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 50 (1958), S. 144-148 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present work is a first attempt at calculating the absolute amount of the motive force responsible for the rotational protoplasmic streaming. The calculation was made on the basis of the conclusion we arrived at previously through the analysis of intracellular velocity distribution, namely, that the active driving mechanism responsible for the rotational streaming is located at the interface between the cortical gel and the outer edge of the endoplasmic layer. The motive force, which is the shifting force generated at this interface, was determined in the internodal cell ofNitella flexilis to be within the range of 1–2 dynes/cm2 at room temperature.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 33 (1939), S. 427-439 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 48 (1957), S. 94-112 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The motive force responsible for the protoplasmic streaming in the myxomycete plasmodium,Physarum polycephalum, was measured by means of the double-chamber method under the influence of various chemical agents. 2. The motive force rather increases than decreases under anaerobic state as well as under the effect of KCN. The process is reversible. 3. Monoiodoacetic acid and sodium fluoride, both fermentation poisons, decrease the strength of the force back of the protoplasmic streaming. 4. 2,4-dinitrophenol inhibits the motive force production both under aerobic and anaerobic conditions. 5. ATP admitted from without markedly increases the motive force of the protoplasmic streaming. The effect is manifested several minutes after the reagent is applied and continues several ten minutes after the reagent is removed. 6. As the fermentation poisons depresses the generation of the motive force while conditions inhibiting respiration do not depress it, it was concluded that the direct energy source of the protoplasmic streaming is ATP synthesized by fermentation process.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 32 (1939), S. 373-396 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 53 (1961), S. 600-614 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 39 (1950), S. 333-343 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The electrical response released by the slime mold,Physarum polycephalum, through mechanical and electrical stimuli disobeys the all-or-none law. 2. A stimulating current flowing outwards through the surface of the protoplasm is more effective in evoking a response than a current flowing in the opposite direction. 3. The process underlying electrical responses is the same irrespective of the means of stimulation, either mechanical or electrical. 4. The strength-duration relation agrees well with the Weiss' formulao=b (1+k/t, in whichb (rheobase) is 2–10 volts andk (chronaxie) is approximately 1 millisecond. 5. The “action potential” of the slime mold accompanies a simultaneous change in the electric impedance of the protoplasm. There is always a close parallelism between them. 6. Protoplasmic flow can proceed unhindered at the moment when electrical responses are induced.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 39 (1950), S. 344-357 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The manner of the locomotion of the slime mold,Physarum polycephalum, was shown graphically using a double-chamber volumeter developed by the author. It enabled him to represent in undulating curves every detail of the way in which the slime mold moves on little by little by availing itself of the difference in transport-volume of the endoplasm produced at each repetition of the back and forth streaming. The curve showing the locomotion of the organism pointed out that more than 4 mm3 of protoplasm is sometimes shifted in a direction in one streaming duration. No close relationship is found between the streaming duration and the transport-volume of protoplasm. The intensity of the flow, which may be defined as the volume of protoplasm transported per unit time, can be obtained from the transport-volume curve through its graphical differentiation.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 65 (1968), S. 358-362 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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