ISSN:
1476-4687
Source:
Nature Archives 1869 - 2009
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Notes:
[Auszug] Physarum actin and myosin were purified as reported previously3. Actomyosin (molar ratio 1:1) was reconstituted by mixing actin and myosin in 20 mM imidazole buffer of pH 7.0, 30 mM KC1, 0.1 mM EDTA and 0.2 mM dithiothreitol (DTT). After centrifugation at 30,000g for 15 min, the precipitated ...
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/285169a0
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