ISSN:
0006-3592
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Polymer ampholites, partially N-succinylated chitosans (PSC), and partially N-succinylated glycolchitosans (PSGC) were prepared from chitosan (an N-deacetylated chitin) and glycolchitosan (a partially O-2-hydroxyethylated chitosan), and they were utilized as novel supports for the immobilization of enzymes. The immobilization was conducted simultaneously with gelation of PSC and PSGC by reaction with water-soluble carbodiimide in the presence of enzymes. Enzymes were covalently bonded on PSC and PSGC chains. Maximum activity yields of glucoamylase, β-fructosidase, and D-glucose isomerase were 58.8, 64.3, and 65.2%, respectively. Favorable activity yields of glucoamylase and β-fructosidase were attained with PSC and PSGC having high degree of N-succinylation, but those of D-glucose isomerase were not affected by the degree of N-succinylation (DS). The activity of immobilized glucoamylase was retained up to 85.5% over 30 batch reactions.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bit.260240505
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