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  • 1
    Electronic Resource
    Electronic Resource
    c-mos immunoreactivity is an indicator of good prognosis in lung cancer (2000)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Histopathology 37 (2000), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Reports concerning the expression of cytoplasmic components of the mitogen-activating protein kinase (MAPK) pathway in lung cancer are limited. One of the molecules participating in this pathway is the product of the c-mos proto-oncogene. In vitro investigations, in somatic cells, have shown that c-mos expression has opposing effects on cell cycle progression suggesting that it may represent an important determinant of aberrant cell function. In this study we analysed, by immunohistochemical means, its status in a series of lung carcinomas and correlated the findings with clinicopathological parameters and survival of the patients.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and resultsSixty cases of lung carcinomas were included in the study. These comprised 52 non-small (NSCLCs) and eight small cell lung carcinomas (SCLCs). Sections from the carcinomas were immunostained with the polyclonal anti-c-mos antibody P-19. Specificity was tested by using the appropriate control peptide and control cell lines. Expression was observed in 63% of the cases, with NSCLCs showing higher reactivity (67%) than SCLCs (37.5%). Staining was observed mainly to the cytoplasm and membranes of the cancerous cells, but some nuclei reacted as well. An intratumour heterogeneous immunoreactivity was noticed. The most interesting and unexpected finding was that c-mos positive staining was associated with better recurrence-free survival in our series, regardless of histological type (P = 0.035). Furthermore, favourable disease-related and recurrence-free survival was observed in the SqC group with c-mos immunoreactivity (P 〈 0.001).〈section xml:id="abs1-3"〉〈title type="main"〉Conclusions c-mos proto-oncogene is expressed in a significant proportion of lung carcinomas and may play a role in its development. The fact that its expression is associated with a relatively good prognosis may be indicative of a negative impact on tumour growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2000.00898.x
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  • 2
    Electronic Resource
    Electronic Resource
    Hyalinosis cutis et mucosae: excessively secreted basal lamina by pericytes and myofibroblasts and its conversion to hyalin (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of the European Academy of Dermatology and Venereology 17 (2003), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1468-3083.2003.00519_8.x
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  • 3
    Electronic Resource
    Electronic Resource
    Elevated serum levels of the apoptosis related molecules TNF-α, Fas/Apo-1 and Bcl-2 in oral lichen planus (2004)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 33 (2004), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  The serum circulatory levels of apoptosis related molecules measured in patients with oral lichen planus (OLP) and healthy individuals in order to investigate possible alterations associated with the clinical forms of OLP.Methods:  Serum levels of tumor necrosis factor (TNF)-α, soluble Fas (sFas) and Bcl-2 studied by enzyme-linked immunosorbent assay in whole blood samples in 13 OLP reticular, 13 OLP atrophic-erosive form patients and 26 healthy subjects.Results:  Significantly elevated levels of TNF-α and sFas detected in OLP patients as compared with controls. Serum concentrations of Bcl-2 although increased in 17/26 patients, they were not statistically significant. Reticular OLP exhibited slightly elevated TNF-α and significantly elevated Bcl-2 serum levels, compared with erosive OLP.Conclusions:  These data suggest that a putative dysfunction in the Fas/FasL mediated apoptosis might be involved in the OLP pathogenesis. A downregulation of Bcl-2 serum levels in the atrophic-erosive OLP may be associated with promotion of the disease activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.2004.00221.x
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of p53 protein in laryngeal squamous cell carcinoma and dysplasia: possible correlation with human papillomavirus infection and clinicopathological findings (1994)
    Springer
    Virchows Archiv 425 (1994), S. 481-489 
    Details
    ISSN: 1432-2307
    Keywords: p53 ; Human papillomavirus ; Polymerase chain reaction ; In situ hybridization ; Laryngeal squamous cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to evaluate the expression of p53 protein in 28 premalignant and 40 malignant squamous cell proliferations of the larynx and its relationship to tobacco consumption, human papillomavirus infection and differentiation grade of the lesions, p53 expression was examined by means of a microwave post-fixation immunohistochemical method using the PAb 240 and PAb 1801 monoclonal antibodies. HPV infection was assessed by non-isotopic in situ hybridization (NISH) and polymerase chain reaction (PCR). A large proportion of carcinomas (77.5%) and dysplasias (61%) expressed p53. No difference was found between differentiation grades of the lesions regarding p53 detection (P〉0.1), but moderate or intense p53 expression was more frequent in the carcinomas (P〈0.05). A statistical correlation was found between cigarette consumption and both p53 detection and p53 staining intensity (P〈0.05 in each case). HPV study revealed HPV 16 and 18 infection only in carcinomas. The frequency was 28% and the physical state of the virus as demonstrated by NISH was integration into the genome. We observed an inverse relationship between HPV infection and p53 expression (P=0.006). Our findings suggest that p53 overexpression is a common and early event which increases in frequency with progression of laryngeal squamous cell carcinoma. The expression of p53 is influenced by tobacco and high-risk types of HPV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197551
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular analysis of p53 gene in laryngeal premalignant and malignant lesions. p53 protein immunohistochemical expression is positively related to proliferating cell nuclear antigen labelling index (1995)
    Springer
    Virchows Archiv 426 (1995), S. 339-344 
    Details
    ISSN: 1432-2307
    Keywords: p53 mutations ; Polymerase chain reaction ; Single strand conformation polymorphism ; Proliferating cell nuclear antigen ; Laryngeal squamous cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was undertaken in order to investigate the molecular nature of the p53 gene in 19 laryngeal squamous cell carcinomas and dysplasias. Moreover, we have examined the possible relationship between proliferating cell nuclear antigen (PCNA) expression and p53 protein detection status in 42 laryngeal premalignant and malignant lesions in which 14 of the 19 samples used in the molecular study were included. p53 gene analysis was performed with the single-strand conformation polymorphism technique. PCNA was stained with the peroxidase/antiperoxidase immunohistochemical method using the monoclonal antibody PC-10. Data from previous work concerning p53 expression was used. We found that 9 of 12 of the immunohistochemically p53 positive (+) cases had mutations in exons 5 or 6. In the remaining immunohistochemically p53(+) and p53 negative (−) specimens there was no indication of sequence alterations. Furthermore, we did not observe any deletions in the chromosomal region 17p31.1 which encodes exons 4–8 of the p53 gene. The PCNA labelling index (LI) increased progressively with p53 protein detection status (percentage of cells immunohistochemically positive for p53). The difference between the group with the higher percentage of p53(+) cells and the others was statistically significant. These data show that although there is a discrepancy between immunohistochemical demonstration of p53 and molecular analysis, a large proportion of the former harbours the mutant form of the protein. In addition, p53 overexpression is positively correlated with PCNA LI, a finding which accompanies tumour progression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191341
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  • 6
    Electronic Resource
    Electronic Resource
    Epidermal growth factor receptor expression in squamous cell lung carcinomas: An immunohistochemical and gene analysis in formalin-fixed, paraffin-embedded material (1993)
    Springer
    Virchows Archiv 423 (1993), S. 295-302 
    Details
    ISSN: 1432-2307
    Keywords: Epidermal growth factor receptor ; Squamous cell lung carcinoma ; Southern blot ; Differential polymerase chain reaction ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Epidermal growth factor (EGF) and its receptor (EGFr) constitute an important and well-characterized mitogenic system in various ectodermal tissues. We evaluated the expression of EGFr and examined possible EGFr gene alterations in 18 formalin-fixed, paraffin-embedded squamous cell lung carcinomas (SCLC) by an immunohistochemical assay, Southern blotting and differential polymerase chain reaction (DPCR). The immunohistochemical study employing the F4 and EGF-R1 monoclonal antibodies, directed against the intra- and extra-cellular portion of the receptor respectively, showed EGFr over-expression in 89% of the SCLC cases examined. All cases showed positive immunostaining for both antibodies, thus excluding the possibility of truncated receptors. In addition, analysis of the EGFr gene was carried out by Southern blotting and DPCR on paraffin extracted DNA from the same carcinoma cases. We found amplification of the EGFr gene in 5/18 (27%) SCLCs. All 5 positive cases showed EGFr over-expression, suggesting a possible correlation between the presence of EGFr gene amplification and over-expression of receptor protein. No correlation was observed among EGFr staining, EGFr gene amplification and differentiation of carcinomas. In addition, Southern blot analysis with HER-A2, a probe which hybridizes a sequence of the receptor's intracellular domain, revealed three novelEcoRI restriction fragment patterns. We suggest that these patterns correspond toEcoRI polymorphic sites of the receptor's tyrosine kinase domain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606894
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  • 7
    Electronic Resource
    Electronic Resource
    The use of laser surgery in subtotal meniscectomy and the effect of low-level laser therapy on the healing potential of rabbit meniscus: an experimental study (1996)
    Springer
    Lasers in medical science 11 (1996), S. 109-115 
    Details
    ISSN: 1435-604X
    Keywords: Subtotal meniscectomy ; CO2 laser ; Diode laser ; Healing potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract The aim of this study was to evaluate the effect of low-level laser therapy on the healing potential of the peripheral rim of rabhit meniscus following laser subtotal meniscectomy, and to compare the subtotal meniscectomy as produced by a surgical blade and by a surgical CO2 laser. Twenty-four male New Zealand rabbits were divided into three groups (A, B and C). In each animal, a subtotal meniscectomy of the medial meniscus of the right knee was carried out. In Group A, meniscectomy was performed with a surgical blade, and in Groups B and C, a meniscectomy was performed with a CO2 laser (6 W, 0.1 mm, 0.2 s). The animals of Group C were treated every other day with a GaAIAs diode laser (820 nm, 100 mW, 48 J cm−2) until death. Two rabbits of each group were killed on the 10th, 20th, 30th and 40th post-operative days. The medial meniscus was removed from all cases, fixed in buffered formalin and embedded in paraffin wax. Subsequently, the sections were stained with Haematoxylin and Eosin as well as with Masson's trichrome, and examined microscopically and morphometrically. This study showed that: (1) fibroblasts and collagen fibrils were more abundant and dense in rabbits of Group C than in rabbits of Groups A and B; (2) after the 20th post-operative day, the granulation tissue was more cellular in Group C in comparison with Groups A and B; and (3) the morphometric evaluation showed that the subtotal meniscectomy with CO2 laser in Groups B and C caused less tissue damage in comparison with the conventional surgical technique used in Group A. It was concluded that: (1) less damage was produced by a CO2 laser than by a surgical blade; and (2) the healing potential of rabbit meniscus following laser resection was accelerated with the use of low-level laser therapy post-operatively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133207
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