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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Agriculture, Ecosystems and Environment 47 (1993), S. 223-235 
    ISSN: 0167-8809
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Exposure of the moderately halophilic bacterium, Deleya halophila, to high NaCl concentrations (2 or 2.5 M) resulted in a transient cessation of cell division. The time taken for the cells to adapt and grow depended on the final salt concentrations. During the initial phases of adaption to high salt both the rate of protein synthesis and amino acid uptake were transiently inhibited. The extent and duration of the inhibition was dependent on the magnitude of the salt shock. Alterations in the patterns of pulse-labelled proteins were observed during adaption to high salt. The response of Deleya halophila cells to decreasing salinity (2.5 to 1 M NaCl) was also characterized by distinct changes in the protein profiles, whereas minor changes in the protein patterns were observed during adaptation from 1 M to 0.5 M NaCl. The labelled protein patterns of cells grown in 1 M or 2.5 M NaCl appear to be similar but not identical.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 48 (1992), S. 54-57 
    ISSN: 1420-9071
    Keywords: Deleya halophila ; oxidative stress ; viability ; protein synthesis ; heat shock
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The sensitivity ofDeleya halophila to oxidative stress caused by hydrogen peroxide (H2O2) was found to vary, depending on the NaCl concentration of the growth medium. Pretreatment of the bacteria at a low concentration of H2O2 (50 μM) protected the cells against the lethal effects of higher levels (1–2 mM) of H2O2. Exposure ofD. halophila cells to 50 μM H2O2 resulted in the induction of several proteins (hydrogen peroxide-inducible proteins, hips). However, the kinetics of induction, the extent of induction and the number of hips appear to be influenced by the salt concentration of the growth medium. Five of the hips exhibited apparent molecular masses identical to those of five heat shock proteins (hsps).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 51 (1995), S. 90-94 
    ISSN: 1420-9071
    Keywords: Uricase II ; Phaseolus ; nodulin ; nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract APhaseolus vulgaris uricase II cDNA clone has been isolated and sequenced. Comparison on the nucleotide level between this clone and the soybean uricase II clone revealed 88.8% identity. The in situ hybridization technique was used to follow the expression pattern in developing root nodules ofPhaseolus vulgaris. The uricase II transcripts were localized only in the uninfected cells of the central tissue and mainly in the periphery of the cell. Uricase II mRNA is first detected in nodules 12 days after infection. A maximum level of transcripts is reached in 21-day-old nodules, followed by a considerable reduction in 28-day-old nodules.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5028
    Keywords: pea ; peribacteroid space ; protein excretion ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-dimensional gel electrophoresis. Silver staining revealed approximately 40 PBS polypeptides. Ex planta 35S-methionine labeling of PBM-enclosed bacteroids revealed that about 90% of the PBS proteins are synthesized by the bacteroid. Approximately 30% of the PBS polypeptides are common between the PBS and the periplasmic space of free-living bacteria; one (38kDa) PBS protein is also excreted by free-living bacteria in the bacterial culture medium. At least four bacteroid-encoded PBS polypeptides were clearly identified as symbiosis-specific.
    Type of Medium: Electronic Resource
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