Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
23 (1974), S. 0
ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Squid axonal preparations consisting of the giant axon plus adhering small nerve fibres were incubated for 30 min with phospholipase A (1, 0.2 and 0.025 mg/ml); phospholipase C (10 and 0.5 mg/ml) or lysolecithin (1 and 0.2 mg/ml) followed by another 30 min incubation in normal sea water. The axoplasm and envelope (sheath) of the axonal preparation were then separated and the phospholipids and free amino acids determined. The released amino acids were also measured in the incubation solutions. Compared to phospholipase C, phospholipase A caused a much greater reduction in the free amino acid content of axoplasm and envelope; and a concomitant much greater increase in amino acids released into the incubation solutions, even when phospholipase A was used in concentrations which caused less phospholipid splitting than phospholipase C. Lysolecithin had a much weaker effect than phospholipase A. It is concluded that disruption of hydrophobic binding has a much greater effect on the structure of the non-lipid portion of the axonal membranes than does disruption of hydrophilic (electrostatic) forces of interaction. Our results can be interpreted in terms of the protein-crystal or mosaic models of membrane structure whereas they do not support the unit membrane hypothesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1974.tb06915.x
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