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Proceedings of the Australian Physiological and Pharmacological Society Symposium: New Frontiers in Muscle Research Gene transfer: manipulating and monitoring function in cells and tissues (2001)

Panchal, Rekha G ; Williams, David A ; Kitchener, Peter D ; [et al.]

Melbourne, Australia : Blackwell Science Pty

Clinical and experimental pharmacology and physiology 28 (2001), S. 0

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ISSN: 1440-1681

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: 1. The ectopic expression of genes has proven to be an extremely valuable tool for biologists. The most widely used systems involve electrically or chemically mediated transfer of genes to immortalized cell lines and, at the other end of the spectrum, transgenic animal models. As would be expected, there are compromises to be made when using either of these broad approaches. Immortalized cell lines have limited ‘physiological relevance’ and transgenic approaches are costly and out of the reach of many laboratories. There is also significant time required for the de novo generation of a transgenic animal.2. As a viable alternative to these approaches, we describe the use of recombinant adenovirus and Sindbis virus to deliver genes to cells and tissues.3. We exemplify this approach with studies from our laboratories: (i) an investigation of Ca2+ handling deficits in cardiac myocytes of hypertrophied hearts using infection with recombinant adenovirus encoding either green fluorescent protein (GFP) or the sarcoplasmic/endoplasmic reticulum calcium-ATPase (Serca2a); (ii) a study of the mechanism of macrophage/microglial migration by infection of embryonic phagocytes with a GFP-encoding virus and coculture with brain slices to then track the movement of labelled cells; and (iii) we are also exploiting the natural tropism of the Sindbis virus to label neurons in hippocampal brain slices in culture to resolve high-resolution structure and to map neuronal connectivity.4. Further development of these approaches should open new avenues of investigation for the study of physiology in a range of cells and tissues.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1440-1681.2001.03504.x

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The optimal dimensions of convective-radiating circular fins (1999)

Khan, Jameel-ur-Rehman ; Zubair, S. M.

Springer

Heat and mass transfer 35 (1999), S. 469-478

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ISSN: 1432-1181

Source: Springer Online Journal Archives 1860-2000

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: Abstract The optimal dimensions of convective-radiating circular fins with variable profile, heat-transfer coefficient and thermal conductivity, as well as internal heat generation are obtained. A profile of the form y=(w/2) [1+(r o/r) n ] is studied, while variation of thermal conductivity is of the form k=k o[1+ɛ((T−T ∞)/ (T b−T ∞)) m ]. The heat-transfer coefficient is assumed to vary according to a power law with distance from the bore, expressed as h=K[(r−r o)/(r e−r o)]λ. The results for λ=0 to λ=1.9, and −0.4≤ɛ≤0.4, have been expressed by suitable dimensionless parameters. A correlation for the optimal dimensions of a constant and variable profile fins is presented in terms of reduced heat-transfer rate. It is found that a (quadratic) hyperbolic circular fin with n=2 gives an optimum performance. The effect of radiation on the fin performance is found to be considerable for fins operating at higher base temperatures, whereas the effect of variable thermal conductivity on the optimal dimensions is negligible for the variable profile fin. It is also observed, in general, that the optimal fin length and the optimal fin base thickness are greater when compared to constant fin thickness.