ISSN:
1573-8221
Keywords:
lymphocytopoiesis
;
clone formation
;
lymphocyte receptors
;
immunoglobulins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Antigen-binding cell clones and Ig-positive cells were found and quantitatively assessed in primary hematopoietic splenic colonies. The results were analyzed on the basis of the hypothesis, the validity of which is discussed, that the ratio of clone volumes of specialized B-cells should reflect the quantitative ratio between the corresponding V-genes in a given lymphocyte population at certain stages of its development. The colonies differed markedly from one another in their curves of inhibition of rosette formation with DNP-erythrocytes by DNP-lysine, i.e., in the avidity of the B-cells of the given specificity. The colonies differed markedly also in the ratio between the volumes of the two clone groups studied (cells with anti-DNP and anti-BE Ig-receptors) with each other and with the total population of Ig-positive cells. These quantitative relationships were incompatible with the view that every B-cell contains every conceivable V-gene of the set, i.e., with the germ-line hypothesis of diversity of antibodies and receptors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00797143
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