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  • 1
    Electronic Resource
    Electronic Resource
    No relationship between genetic instability in Bloom's syndrome and DNA hypomethylation of some major repetitive sequences (1993)
    Springer
    Human genetics 〈Berlin〉 92 (1993), S. 57-60 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bloom's syndrome (BS) is an autosomal recessive disorder, characterized by a high incidence of cancer at a young age. Cytogenetically, BS cells exhibit a high frequency of chromosomal damage and sister chromatid exchange (SCE). Thus, BS provides a human model of a genetic disorder exhibiting both chromosomal instability and a high incidence of cancer. In addition to its involvement in gene regulation, CpG methylation has recently been suggested to play an important role in the evolution and stability of chromosome structure. We have examined DNA methylation profiles of total DNA and some selected repeated sequences in normal and BS cells. No specific DNA hypomethylation in either total blood or lymphoblastoid cell lines from BS patients has been detected, suggesting that the genomic instability observed in BS is not directly related to a major DNA demethylation of the total CCGG sites, or of Alu or chromosome 1 satellite 2 repeated sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216145
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Insulin effect on GABA uptake in astroglial primary cultures (1988)
    Springer
    Neurochemical research 13 (1988), S. 1119-1124 
    Details
    ISSN: 1573-6903
    Keywords: Insulin ; astroglia ; cultures ; GABA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Astroglial cultures from newborn mouse cerebral cortex contain [125I]insulin binding sites. Binding was specific reversible, time dependent and reached equilibrium after 45 min. Insulin analogues compete for this [125I]Insulin binding. Incubation of cerebral cortex astroglial cultures with insulin induced a time-and dose-dependent inhibition of the [3H]GABA high affinity uptake. A decrease in theV max rather than, an effect on theK m was observed. This effect was dose-dependent and effective at 10−10 M. Autoradiographic observations on the cell monolayer showed the presence of two groups of cells: one which strongly takes up [3H]GABA and consist in smaller GFAP positive process-bearing cells and another group of much flatter and larger GFAP positive cells which uptake was lower. The smaller stellate cells were apparently the most sensitive to insulin effect. These results: 1) confirm the presence of insulin binding sites on astroglial primary cultures, 2) show an effect of insulin of [3H]GABA high affinity uptake of these cells; this effect being optimal on a stellate-like population of astrocytes, and 3) indicate, that insulin may interfere in neuromodulation through astroglial signals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00971628
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    Paper (German National Licenses)
    Fulltext
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