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  • 1
    Electronic Resource
    Electronic Resource
    Spatial distribution of mammalian cells grown on macroporous microcarriers with improved attachment kinetics (1992)
    s.l. : American Chemical Society
    Biotechnology progress 8 (1992), S. 486-493 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00018a003
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of serum type and concentration on the expression ofβ-galactosidase in recombinant vaccinia virus infected HeLa S3 cells (1995)
    Springer
    Cytotechnology 19 (1995), S. 153-159 
    Details
    ISSN: 1573-0778
    Keywords: vaccinia virus ; HeLa S3 cells ; β-galactosidase ; calf and horse serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of serum type and concentration on recombinant protein expression in vaccinia virus infected HeLa S3 cells was studied in both static and suspension culture. A model heterologous protein,β-galactosidase (β-gal), was used. Calf and horse sera in the range of 0.5–10%(v/v) were investigated. In static culture, the calf serum concentration did not show any significant influence on the β-gal production which was almost completed within 24h postinfection (pi). Higher horse serum concentration, on the other hand, resulted in higher β-gal concentration which continued to increase until 48 h pi. Total β-gal concentrations in 0.5% calf serum at 24 h pi and 10% horse serum at 48 h pi were 2.2±0.7 and 2.2±0.1 IU/ml, respectively. In suspension culture, both sera showed their respective effects on the β-gal production similar to those observed in static culture, indicating that the cultivation method had little influence on β-gal production. Accordingly, the use of 0.5% calf serum after virus infection in recommended for economical β-gal production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749770
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of calcium chloride treatment on hybridoma cell viability and growth (1992)
    Springer
    Biotechnology letters 14 (1992), S. 891-896 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and treatment time. The cell viability as measured by trypan blue exclusion did not decrease rapidly during the first hour of calcium chloride treatment regardless of calcium chloride concentrations used (1.3 and 1.5%). However, 1.3% calcium chloride solution appeared to be more detrimental to the cells than 1.5% calcium chloride solution. The cells in 1.3% calcium chloride solution lost their viability faster than the cells in 1.5% calcium chloride solution. In addition, when the cells treated with calcium chloride were inoculated into spinner flasks containing IMDM with 10% fetal calf serum, the cells treated with 1.3% calcium chloride solution showed a longer lag phase than the cells treated with 1.5% calcium chloride solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01020624
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  • 4
    Electronic Resource
    Electronic Resource
    Production of a polysaccharide, methylan, in Methylobacterium organophilum by controlling ammonium ion (1996)
    Springer
    Biotechnology letters 18 (1996), S. 1427-1430 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cell growth increased proportionally to the initial concentration of ammonium ion, however, methylan production was significantly inhibited at the high concentration of ammonium ion. The control of ammonium ion within the desired level(usually 0.45 g/l) was needed to reduce the inhibition. Methylan production was increased to 12.5 g/l by maintaining ammonium ion below 0.15 g/l.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129348
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of cell disruption methods for determining β-galactosidase activity expressed in animal cells (1994)
    Springer
    Biotechnology techniques 8 (1994), S. 425-430 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To determine β-galactosidase activity expressed in animal cells, the cells are often disrupted. In this study, four different cell disruption methods (freezing and thawing, sonication, homogenization, and lysis buffer) were compared with regard to their efficiency in the determination of β-galactosidase activity. Further, the cell lines' susceptibility to cell disruption methods was investigated by employing three different animal cell lines (HeLa HH, Vero, and HeLa S3 cells) infected by recombinant vaccinia virus (vSC8) expressing β-galactosidase gene. Regardless of cell lines used, three different cell disruption methods except homogenization did not show any significant difference in the final β-galactosidase activity recovered from the cells. Homogenization was inefficient, and required up to 300 strokes to recover β-galactosidase activity fully from HeLa S3 cells. The use of lysis buffer was recommendable because of its convenience. However, if there was no need for an immediate assay, it was convenient to keep cell samples collected during the culture frozen at −20°C until the assay. Since thawing of the cells was enough to recover the β-galactosidase, further treatment on the frozen cells after thawing was unnecessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154315
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  • 6
    Electronic Resource
    Electronic Resource
    Metal adsorption of the polysaccharide produced from Methylobacterium organophilum (1996)
    Springer
    Biotechnology letters 18 (1996), S. 1161-1164 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Metal adsorption of the acidic polysaccharide produced from Methylobacterium organophilum was investigated. The polysaccharide non-specifically adsorbed 21% for copper and 18% for lead of the polysaccharide used after 30 minutes of reaction time at pH 7. The metal adsorption may be caused by the interaction between metal cations and negative charges of acidic residues of the polysaccharide such as uronic, pyruvic and acetic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128585
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  • 7
    Electronic Resource
    Electronic Resource
    Erythritol production by controlling osmotic pressure in Trigonopsis variabilis (1997)
    Springer
    Biotechnology letters 19 (1997), S. 727-729 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Specific erythritol production rate by Trigonopsis variabilis increased from 0.09 to 0.19 g/g-day by varying the osmotic pressure from 1.3 to 3.9 kPa in glucose medium, but the specific growth rate decreased from 0.28 to 0.13 h -1 . Therefore, osmotic pressure was adjusted to 1.4 kPa during growth phase and to 3.7 kPa during production phase in a two-stage fermentation. Erythritol reached 46 g/ l in such a system and was twice that obtained in one-stage fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018371722456
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  • 8
    Electronic Resource
    Electronic Resource
    Evaluation of xylitol production from corn cob hemicellulose hydrolysate by Candida parapsilosis (1999)
    Springer
    Biotechnology letters 21 (1999), S. 891-895 
    Details
    ISSN: 1573-6776
    Keywords: Candida parapsilosis ; corn cob hemilcellulose hydrolysate ; xylitol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Candida parapsilosis was grown for 59 h in a medium containing corn cob hydrolysate consisting of 50 g xylose l−1, 3.0 g glucose l−1, 2.0 g arabinose l−1, and 0.9 g acetic acid l−1. A biomass of 9.1 g l−1 was produced with 36 g xylitol l−1 and 2.5 g ethanol l−1. In a medium containing 50 g xylose l−1 instead of corn cob hydrolysate, the concentrations of cells, xylitol, and ethanol were 8.6 g l−1, 33 g l−1, and 0.2 g l−1, respectively. The differences between two cultures were due to the glucose and arabinose in the corn cob hydrolysate stimulating growth and the low concentration of acetic acid stimulating xylitol production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005574624617
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  • 9
    Electronic Resource
    Electronic Resource
    Relationship between emulsifying activity and carbohydrate backbone structure of emulsan from Acinetobacter calcoaceticus RAG-1 (1997)
    Springer
    Biotechnology letters 19 (1997), S. 457-459 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Various emulsan samples with the different degrees of branching of the carbohydrate backbone were obtained from Acinetobacter calcoaceticus under different culture conditions. The emulsifying activity of emulsan had a linear correlation to the branching degrees of the carbohydrate backbone (r 2 = 0.930) suggesting that the structure of carbohydrate backbone was an important factor in_uencing emulsifying activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018348227965
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  • 10
    Electronic Resource
    Electronic Resource
    Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation (1989)
    Springer
    Cytotechnology 2 (1989), S. 135-140 
    Details
    ISSN: 1573-0778
    Keywords: hepatoma ; cell culture ; microcarrier ; agitation rate ; cell inoculation density ; growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386146
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