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  • 1
    Electronic Resource
    Electronic Resource
    Clinicopathologic Correlation of p53 Protein Overexpression in Adenoma and Carcinoma of the Ampulla of Vater (2000)
    Springer
    World journal of surgery 24 (2000), S. 54-59 
    Details
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. It has been well documented that ampullary carcinoma arises from a precancerous lesion, but there have been few studies concerning changes at the molecular level during the adenoma-carcinoma sequence. In this study, p53 overexpression during the progression of carcinoma was compared and the relation between p53 expression and prognosis was analyzed. Ninety-four cases of adenocarcinoma of the ampulla of Vater were reviewed histopathologically and examined for overexpression of p53 protein using the DO-7 (mouse monoclonal; DAKO, Glostrup, Denmark) antibody. The correlation of p53 overexpression with the existence of adenoma, clinical stage, histologic grade, and overall survival was investigated. The proportion of p53-positive cases among normal mucosa, adenoma, early stage carcinoma (I and II), advanced stage carcinoma (III and IV), and metastatic lesion was 0% (0/94), 14.3% (6/42), 32.3% (20/62), 53.1% (17/32), and 63.3% (19/30), respectively. The existence of adenoma or histologic grade of carcinoma did not correlate with p53 overexpression. The carcinoma having adenomatous component was more common in early stages (54.8% in stages I and II, 25% in stages III and IV; p= 0.006) and in well-differentiated carcinoma (p= 0.001). The existence of adenoma or p53 overexpression did not independently correlate with prognosis. In contrast, the p53 overexpressed group without adenoma showed a worse prognosis than the remaining patients (p= 0.0006) and this trend was still demonstrable when the groups were compared stage by stage. In ampullary carcinoma, p53 abnormality occurs during malignant transformation from the adenoma and continues during the tumor progression in carcinoma. The clinical prognosis of de novo carcinomas with p53 overexpression was worse than that of the remaining patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002689910011
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  • 2
    Electronic Resource
    Electronic Resource
    A Study on the Method of Vibration Analysis for Korean High Speed Train by the On-Line Test (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 321-323 (Oct. 2006), p. 1593-1596 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The Korean High Speed Train (KHST) has been tested on the Kyongbu high speed line andthe Honam conventional line since 2002. A data acquisition system was developed to test and provethe dynamic performance of the KHST, and the system has been found to be very efficient inacquiring multi-channel data from accelerometers located all over the train. Also presented in thispaper is an analysis procedure which is simple and efficient in analyzing the acceleration dataacquired during the on-line test of the KHST. The understanding of system vibration mode for arailway vehicle is essential to evaluate the characteristics of a dynamic system and to diagnose thedynamic problems of the vehicle system during tests and operations. Methods based on homogeneouslinear systems are not realistic because real systems have nonlinear characteristics and are stronglydependent on environmental conditions. In this paper an efficient method of vibration analysis hasbeen proposed and applied for the KHST to evaluate its vibration mode characteristics. The resultsshow that this method is suitable to estimate the system vibration modes of the KHST
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.321-323.1593.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Role of calmodulin in the activation of carbachol-activated cationic current in guinea-pig gastric antral myocytes (1995)
    Springer
    Pflügers Archiv 430 (1995), S. 757-762 
    Details
    ISSN: 1432-2013
    Keywords: Gastrointestinal myocytes ; Nonselective cation channels ; Carbachol ; Calmodulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In mammalian gastrointestinal myocytes, it is known that muscarinic stimulation activates nonselective cation channels through a G-protein and a Ca2+dependent pathway. We recorded inward cationic currents following application of carbachol (I CCh) to guinea-pig gastric myocytes, which were held at −20 mV using the whole-cell patch-clamp method. I CCh was suppressed by nicardipine or removal of Ca2+ from the bath solution. The peak value of inward current induced by repetitive applications of carbachol (CCh) decreased progressively (run-down phenomenon). This run-down was significantly alleviated by the addition of calmodulin to the pipette solution (0.15 mg/ml) or by using the perforated-patch whole-cell voltage-clamp technique. Moreover, W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide], a calmodulin antagonist, was a reversible inhibitor of I CCh. However, W-7 had only a weak inhibitory effect on the same cationic current which was induced by guanosine 5′-O-(3-thiotriphosphate) (GTP[γS] 0.2 mM) in the pipette solution. This GTP[γS]-induced cationic current was still markedly suppressed by the Ca2+-free bath solution. W-7 itself had a weak inhibitory effect on voltage-operated Ca2+ channels as well as the effects on I CCh. These data suggest that multiple Ca2+-dependent pathways are involved in the activation of CCh-gated cation channels in guinea-pig antral myocytes and a Ca2+/calmodulin-dependent pathway would be one of them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386173
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  • 4
    Electronic Resource
    Electronic Resource
    Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of Go protein in guinea-pig gastric myocytes (1998)
    Springer
    Pflügers Archiv 436 (1998), S. 494-496 
    Details
    ISSN: 1432-2013
    Keywords: Key words Carbachol (CCh) ; G protein ; Nonselective cationic current ; Smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  In this study, we investigated which subtype of GTP-binding protein (G protein) is related to muscarinic activation of nonselective cation (NSC) channels in gastric smooth muscle. Inward cationic current was activated by the application of 50 µM carbachol (I CCh) at a holding potential of –60 mV with the same CsCl-rich solution in both pipette and bath. The same cationic current as I CCh was slowly activated by the dialysis of guanosine 5′-O-(3-thiotriphosphate) (GTP[γ-S]) through the pipette. Since it is known that pertussis toxin pretreatment can block I CCh, antibodies (Abs) against Gα,i (anti-Gα,i) or Gα,o (anti-Gα,o) were tested. Activation of I CCh was blocked by the addition of anti-Gα,o. However, anti-Gα,i Abs had no significant effect on I CCh.The expression of Gα,o in guinea-pig gastric smooth muscle was confirmed by Western immunoblot analysis. These results suggest that Go-type protein may mediate signals from the muscarinic receptor to NSC channel in guinea-pig gastric myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050663
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    Paper (German National Licenses)
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