ISSN:
1437-7780
Keywords:
enterotoxins
;
toxic shock syndrome toxin 1 (TSST-1)
;
polymerase chain reaction (PCR)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The enzymatic detection of a polymerase chain reaction product (ED-PCR), a new detection method of PCR-amplified DNA, was evaluated for the identification of staphylococcal enterotoxin (SE) and toxic shock syndrome toxin 1 (TSST-1) genes. A total of 61Staphylococcus aureus strains, including reference strains and strains isolated from clinical specimens and food poisoning outbreaks, were examined by ED-PCR and by reverse passive latex agglutination (RPLA) phenotypic identification. There was 100% agreement between the genotypic and phenotypic identification of SEA, SEB, SEC, SEE strains and TSST-1. In the case of SED, however, 4 strains were positive by ED-PCR and negative by RPLA. ED-PCR offers an accurate alternative to traditional immunoassays or conventional PCR using electrophoresis for the detection of SE and TSST-1 production yielding results that are more precise than with older techniques.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02350847
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