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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Inflorescence development in tomato plants (Lycopersicon esculentum Mill., cv. King Plus) grown under a low-light regime is promoted by exogenous applications of a mixture of N6-benzyladenine (BA) and gibberellins A4+7 (GA) directly on the inflorescence. The photosynthetic rate of the young mature leaf, which feeds the developing inflorescence, and the proportion of 14C-assimilates exported from the source leaf are not affected by the growth substance treatment, but the pattern of 14C-assimilate distribution is altered. Assimilate supply to the treated inflorescence increases concomitantly with a decrease in the 14C import into the apical shoot, reflecting a competition between these two plant parts. The increased assimilate accumulation in the treated inflorescence is apparent 1 day after the first application of BA+GA, and precedes any morphological changes in the reproductive structure. These results are discussed in relation to nutritional hypotheses that regard assimilate supply as limiting for reproductive development.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mitotic activity and nuclear DNA synthesis in tomato (Lycopersicon esculentum Mill., cv. King plus) flowers targeted for abortion under unfavorable light conditions are completely stopped 6 days after macroscopic appearance of the inflorescence. Ovular cells are arrested at the G1 (80%) and G2 (20%) stages of the cell cycle. Exogenous applications of a mixture of N6-benzyladenine (BA) and gibberellins A4+7 (GA) directly on the inflorescence may prevent its failure. Nuclear DNA synthesis and mitoses resume in ovules of the flower 16 to 20 h after the BA+GA treatment. When applied alone, BA and GA are able to mimic the effect of the mixture upon the progression of ovular cells through their cycle. Sporogenesis processes are also set in motion by the exogenous plant growth regulators. The mechanism of action of cytokinins and gibberellins in the control of floral development is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Carbohydrate ; Flower induction ; Phloem ; sap ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sinapis alba L., a long-day plant, has been induced to flower either by a single 22-h-long photoperiod or by an 8-h short photoperiod displaced by 10 h in a 24 h cycle. The ehtylenediametetraacetate method previously used for leaf exudation was modified to collect phloem sap at the apical part of the shoot. Carbohydrates in the phloem sap have been analysed comparatively in vegetative and induced plants, using high-performance liquid chromatography and refractometry. Sucrose was the major sugar detected. A dramatic increase of its flux in the apical sap occurred early and transiently during the floral transition in plants induced by both long days and displaced short days. These results indicate a message-like role for sucrose since they fit nicely with previous observations indicating that an early event in the floral transition in S. alba is the accumulation of sucrose in the meristem.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Cell division ; Cytokinin ; DNA replication ; Lolium ; Lycopersicon ; Replicon ; Shoot meristem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a previous study (Houssa et al., 1990, Planta 181, 324–326) we observed that a single application of a low dose of benzylaminopurine, a cytokinin, resulted in the halving of the size of the units of DNA replication in the vegetative shoot meristem of Sinapis alba L., a dicotyledonous plant. The effect was due to the recruitment by the hormone of latent replication origins in the chromosomal DNA. Here we report that benzylaminopurine has the same effect in both the vegetative shoot meristem of a monocotyledonous species, Lolium temulentum L., and the tomato (Lycopersicon esculentum Mill.) ovule, a dicotyledonous reproductive meristem. It is thus tentatively concluded that activation of latent replication origins is an universal effect of cytokinins in the regulation of the cell-division cycle.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; aluminium-resistance ; selection ; stress ; in vitro ; hydroponic cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A selection procedure is described that isolated Al-resistant individuals from a sensitive rice (Oryza sativa L.) genotype. Somaclonal variation was used as the only source of variability and selection pressures were applied at both callus and regenerated plant levels. When 10 week-old, embryo-derived calli were submitted to different Al stresses for a period of 20 weeks. After this in vitro stress period, selected calli were grown during 18 weeks, without selection pressure, on regeneration media. Selection pressures were applied on regenerated plants (R0), and 9 R0 plants which produced seeds were selected. The transmission of the Al-resistant character to the R1, R2, R3 and R4 generations was then investigated, and three plant lines which had an increased percentage of Al-resistant plants till the fourth generation of self-pollination, were identified. One was from a callus maintained on an Al-free medium. The efficiency of in vitro selection pressures was therefore debated.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5087
    Keywords: chilling tolerance ; fatty acids ; galactolipids ; phospholipids ; rice ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between chilling tolerance of six rice cultivars – Facagro 57, Facagro 76, Fujisaka 5, Kirundo 3, Kirundo 9 and IR64 -and the fatty acid composition in total lipids, phospholipids, galactolipids and neutral lipids from leaves was studied. Higher double bond index and proportions of linolenic acid in the phospholipid and galactolipid classes were related to cultivar chilling tolerance, but this was not so for the total lipids nor the neutral lipid class. The somaclonal families derived from Facagro 76, Kirundo 3 and Kirundo 9 that showed enhanced chilling tolerance as compared to their original parental cultivar were analyzed for fatty acid composition in phospholipids and galactolipids from leaves. Altered proportions in fatty acid composition in phospholipids, galactolipids or both were found in the somaclonal families derived from Facagro 76 and Kirundo 9, but not from Kirundo 3. These changes most usually resulted in higher double bond index and higher proportions in linoleic and linolenic acids which were related either to lower ratio of C16 to C18 fatty acids or to higher unsaturation in the C18 fatty acid fraction. Different mechanisms thus seem to be implicated in the altered fatty acid composition of somaclones, which may be related to the chilling tolerance improvement of some somaclonal families.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5087
    Keywords: cutting ; defoliation ; flowering ; Lycopersicon esculentum ; meristem identity gene ; season effects ; tomato ; topping ; uniflora mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The uniflora (uf) mutant of tomato (Lycopersicon esculentum Mill.) is known to produce solitary, normal, fertile flowers instead of inflorescences. Histological and SEM studies revealed that this unusual reproductive structure resulted from the inability of the plant to produce an inflorescence and not from post-initiation abortion processes affecting young flower buds. Development prior to floral transition was apparently not affected by the mutation since rates of germination and leaf initiation were identical in both uf and the ‘Ailsa Craig’ (AC) initial cultivar. However, the time of flowering of the mutant was always delayed as compared to AC. In uf, environmental conditions markedly influenced flowering time which occurred early in all individuals in summer, but was strongly delayed during winter, with less than 20% plants reaching flowering before having initiated 40 leaves. Defoliation treatments stimulated floral transition in uf plants since 100% flowering occurred whatever the season and since the time of floral transition was usually advanced in comparison to the non-defoliated control plants. Similarly, compared to intact uf plants, flowering of terminal meristem of cuttings and upper axillary bud of decapitated plants was promoted. The involvement of correlative influences and assimilate availability in the control of flowering in tomato is suggested by these findings.
    Type of Medium: Electronic Resource
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