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  • 1
    ISSN: 1618-2545
    Keywords: carbon dioxide ; Flammulina ; Pleurotus ; fruiting ; mushroom cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Edible basidiomycetesFlammulia velutipes andPleurotus ostreatus were cultivated in the usual manner on media based on sawdust and rice bran, and the cultures were exposed to slowly flowing CO2-enriched air (550 (control), 3,000, 6,000, and 9,000µl/l) for seven days at different stages of cultivation. When the cultures were exposed at the primordium stage (less than 10 mm in length), length and yield of fruit-bodies increased and pileus expansion was slightly inhibited inF. velutipes, while inP. ostreatus length increased, yield decreased, and pileus expansion was greatly inhibited. When the cultures with fruit-bodies larger than 10 mm were exposed, length and yield were insensitive and pileus expansion was greatly inhibited inF. velutipes, while inP. ostreatus length was insensitive, but pileus expansion was heavily damaged by trumpet-like deformation and yield decreased. The different action of CO2 on the two species appeared to be due to the different anatomical structures of their fruit-bodies.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1618-2545
    Keywords: dikaryon-monokaryon matings ; esterase isozymes ; nuclear selection ; nuclear substitution ; Pleurotus ostreatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclear behavior in fully compatible dikaryon-monokaryon matings inPleurotus ostreatus was examined with esterase isozymes as markers, which are located at the EST1 locus independent of mating type factors A and B. Monokaryotic strains mMA91 and mOW89 were obtained from the stocks MA91 from Japan and OW89 from Thailand, respectively. The mMA91 strains represented genotype EST1 (100), and the mOW89 strains represented either EST1 (86) or EST1 (93). Most of the stocks derived from mMA91×OW89 represented the genotype EST1 (93)+EST1 (100). This result suggests that the donor nucleus of OW89 carrying EST1 (93) migrated preferentially into the monokaryon mMA91 to conjugate with the recipient nucleus of the latter. Some of the stocks from mOW89 × MA91 represented the genotype EST1 (100)+EST1 (100). This seems to have resulted from substitution of a nucleus of the recipient mOW89 by a pair of nuclei of the donor MA91. The variations in mycelial products, mycelial growth rate, and wood degrading ability among the D1 populations of stocks from mMA91 × OW89 were significantly smaller than those from mOW89 × MA91, reflecting the selective nuclear migration from OW89 to mMA91.
    Type of Medium: Electronic Resource
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