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  • 1
    Electronic Resource
    Electronic Resource
    Heterogeneity of Protein Profiles of Helicobacter pylori Isolated from Individual Patients (1998)
    Cambridge, MA, USA : Blackwell Science Inc, Boston, USA
    Helicobacter 3 (1998), S. 0 
    Details
    ISSN: 1523-5378
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In order to characterize the diversity of Helicobacter pylori (H. pylori) in infected individuals, 10 colonies of H. pylori were isolated from the gastric juice of 25 patients with gastroduodenal diseases (total 250 isolates).〈section xml:id="abs1-2"〉〈title type="main"〉Methods.Protein profiles of isolates were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results were confirmed by Western blotting (immunoblotting) test using rabbit antisera against three different strains of H. pylori.〈section xml:id="abs1-3"〉〈title type="main"〉Results.The protein profiles of 18 of 25 cases (72%) showed a single type of H. pylori with the same polypeptide pattern. In contrast, heterogeneity in the protein profiles was seen in isolates from seven cases (28%). Two differing H. pylori types with two very different polypeptide patterns were found in 10 isolates from one case. In six patients, the protein profiles of isolates were found to have variations in their polypeptides between molecular weights of 30,000 (30K) and 14K, which are thought to be associated with bacterial membrane protein. In some isolates, a polypeptide band of the 16K was missing. Each of three different antisera confirmed differences among the distinct isolates from individual patients.〈section xml:id="abs1-4"〉〈title type="main"〉Conclusions.These findings suggest that more than one antigenically different strain of H. pylori may exist in same infected individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1523-5378.1998.08703.x
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  • 2
    Electronic Resource
    Electronic Resource
    Purification and partial characterization of Fusobacterium necrophorum haemolysin (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 25 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Extracellular haemolysin of Fusobacterium necrophorum was partially purified by diethylaminoethyl-cellulose column chromatography and Sephadex G-200 gel filtration. The purified preparation was shown to be homogenous by polyacrylamide gel electrophoresis. In sodiumdodecylsulfate-polyacrylamide gel electrophoresis, the haemolysin was divided into two bands. Their Mrs were approximately 54000 and 48000. It was heat-sensitive and oxygen-labile. Inactivated haemolysin in air could be reactivated by the dialysis with ammonium sulfate solution containing cysteine monohydrochloride.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01464.x
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  • 3
    Electronic Resource
    Electronic Resource
    Serum antibody againstHelicobacter pylori assayed by a new capture ELISA (1995)
    Springer
    Journal of gastroenterology 30 (1995), S. 295-300 
    Details
    ISSN: 1435-5922
    Keywords: Helicobacter pylori ; enzyme-linked immunosorbent assay ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed a highly specific detection technique for serum antibody, using a monoclonal antibody to a specific antigen ofHelicobacter pylori. A monoclonal antibody preparation that reacted with the 54-kDa molecule ofH. pylori antigens was obtained. Using this preparation, an antigen-capture enzymelinked immunosorbent assay (ELISA) was established by fixation of the monoclonal antibody, followed by reaction with sonicated whole cell antigens. The serum antibody titers of patients with gastritis and peptic ulcers were significantly higher than control titers, and the antibody titer correlated with the histological severity of gastritis. Patients positive forH. pylori by bacterial culture had higher titers thanH. pylori-negative patients. Our new ELISA may be useful for the diagnosis ofH. pylori infections and for evaluation of the severity of gastric inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02347502
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of immunoreactive antigen, with a monoclonal antibody to measles virus, in tissue from a patient with Crohn's disease (1995)
    Springer
    Journal of gastroenterology 30 (1995), S. 28-33 
    Details
    ISSN: 1435-5922
    Keywords: Crohn's disease ; measles virus ; M protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using immunofluorescence (IF), we investigated reactive antigens present in Crohn's disease patients with monoclonal antibodies derived from cells infected with measles virus, but not with the subacute sclerosing panencephalitis virus. During immunoblotting, one monoclonal antibody (mAb 86) reacted with a polypeptide with a molecular weight of 36 000 dalton (M; matrix protein) in measles virus-infected cells. This monoclonal antibody displayed a positive reaction only with tissues from patients with Crohn's disease by the IF test. It did not react with samples from patients with other chronic inflammatory bowel diseases, such as ulcerative colitis. Other monoclonal antibodies to the measles virus protein, and monoclonal antibodies toHerpes simplex virus type 1 did not react with the same tissue samples. The role of measles virus infection and/or a viral antigen (possibly the M protein) as a causative agent in Crohn's disease poses a challenging avenue for further research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01211371
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  • 5
    Electronic Resource
    Electronic Resource
    Alterations in the sequence of the gene 4 from a human rotavirus after multiple passages in HepG2 liver cells (1993)
    Springer
    Archives of virology 130 (1993), S. 179-185 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The human Wa strain of rotaviruses, initially unable to grow in liver cells, was adapted by multiple passages to grow in HepG2 cells. The genome segment 4 of both the parental and passaged strains was cloned and sequenced. Five amino acid differences (residues 38, 120, 421, 525, and 618) were found in the HepG2-passaged variant compared to the parental Wa strain. Our results support the hypothesis that viral variants that have improved capabilities for infecting liver cells can be generated during infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01319006
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