ISSN:
1600-0536
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The use of a lymphocyte transformation test (LIT] to confirm allergic contact dermatitis from thiurms has been investigated. The responses of peripheral blood mononuclear cell? (PBMC) from thiuram-sensitive; and non-sensitive individuals following culture with dimethylerbamoyl-protein (human serum albumin; USA) and dimethylthiocarbamoly-HSA conjugates has been compared. Only PBMC From those patients who were patch-test-positive with thiuram-mix and sensitized to tetramethylthiuram monosulphide (TMTM) or TMTM and tetramethylthiuram disulphide (TMTD) exhibited significant proliferative responses to these conjugates. Thiuraiu-patch-test.-negative patients and control donors with no history fo allergic contact dermatitis failed to mount a significant response to any concentration of either conjugate. Two of the thiuram sensitive patients were also nickel-patch-test-positive. and PBMC isolated from these donors, but not from nickel-patch-test-negative patients, proved positive in a nickel LTT. The data reveal that relevant hapten-protein conjugates are capable of provoking spicifie human lymphocyte proliferative responses in vitro, and that, using this technique, the LTT can, in principle, be used for the investigation and/or diagnosis of skin sensitization to lipophilic contact allergens.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0536.1991.tb01692.x
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