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  • 1
    Electronic Resource
    Electronic Resource
    Aggregation-promoting factor in human vaginal Lactobacillus strains (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 19 (1997), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A total of 60 Lactobacillus sp. strains were examined for expression of auto-aggregation and cell surface hydrophobicity. Isolates were obtained from the vagina of healthy women (n=20). The results obtained showed that the occurrence of cell surface hydrophobicity correlated with auto-aggregative activity in 12 homofermentative Lactobacillus sp. strains. The aggregation mechanism was mediated by the presence of an aggregation promoting factor (APF) in one Lactobacillus sp. strain, HV 142. APF was confirmed by DNA hybridisation with a 1.3 kb PstI DNA fragment of recombinant plasmid pFDS containing the reading open frame of the APF gene derived from Lactobacillus plantarum 4B2. Coaggregation activity was seen in three strains of auto-aggregative human vaginal lactobacilli and five strains of P-fimbriated uropathogenic Escherichia coli. Moreover, one of four Lactobacillus sp. strains (HV 389) aggregated with two of five E. coli tested. These results suggest that APF producing lactobacilli could represent a further mechanism in the interaction of commensal microflora with strains of uropathogenic E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1997.tb01079.x
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  • 2
    Electronic Resource
    Electronic Resource
    Specific detection of a probiotic Lactobacillus strain in faecal samples by using multiplex PCR (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 158 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Lactobacillus gasseri 4B2 is a human isolate characterised by a strong autoaggregating phenotype mediated by APF (aggregation-promoting factor), a secreted protein. Two primer pairs were developed for simultaneous amplification of a specific fragment of the APF gene and a highly conserved region of the 16S rRNA gene. The specificity of this protocol was checked in DNA samples isolated from single and mixed cultures of Lactobacillus. The same amplification protocol was successfully used directly adding whole bacterial cells to PCR reaction tubes. The suitability of this method for in vivo studies was investigated through feeding L. gasseri 4B2 to mice and analysing colony forming units obtained by plating faecal samples on selective medium. The methodology allows a fast and reliable identification of the target strain without any DNA extraction procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12832.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a new ruminal bacteriophage lytic toStreptococcus bovis (1994)
    Springer
    Current microbiology 28 (1994), S. 355-358 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new bacteriophage, designated F4, was isolated from the ruminal fluid of a calf. The host range of F4 phage was limited to five strains ofStreptococcus bovis out of ten tested on which clear plaques 0.6–1.2 mm in diameter were found. Bacteriophage F4 had an elongated head 75 nm long and 33 nm wide with a noncontractile flexible tail 100 nm in length on average. This phage is defective in the generation of plaques at low multiplicities of infection. Its genome consists of double-stranded linear DNA of 60.38 kb lacking cohesive ends. The F4 DNA was analyzed with 13 restriction enzymes. The restriction enzymes that did not cleave it wereBamHI,EcoRI,PvuI, andSmaI. The circular restriction map was constructed with four restriction endonucleases (XbaI,EcoI,SalI, andBglI).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570201
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    Paper (German National Licenses)
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