ISSN:
1432-1106
Keywords:
Acetylcholine
;
Hemicholinium
;
GABA
;
Thiosemicarbazide
;
Autoradiography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Light microscopic autoradiographic techniques were employed to detect inhibitors of acetylcholine synthesis and of the GABA-metabolism in the rat nervous system. Sites of acetylcholine synthesis were located by demonstrating deposits of in vivo injected 14C-labelled hemicholinium; sites of GABA-metabolism were located by detecting in vivo injected 14C-labelled thiosemicarbazide. 14C-hemicholinium was concentrated in motor end plates, spinal motoneurons, cerebellar cortex, paleo- and neocortex cerebri, and caudate nucleus. Isotope thiosemicarbazide was found in the hippocampus, medial habenular nucleus, cerebellar cortex, dentate nucleus, substantia nigra and nuclei pontis. Localizations of the above enzyme inhibitors are in accord with biochemically demonstrated activities of choline acetylase and glutamic acid decarboxylase, respectively. It is concluded that the acetylcholine-synthesizing machinery is located partly in neuronal parikarya, but mainly in the neuropil. Sites of GABA-metabolism are more variable; they include neuronal parikarya, neuropil and also glial cells, with special predilection to various brain areas.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00234198
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