Bibliothek

X
feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
  • 1
    Digitale Medien
    Digitale Medien
    Neuron-Glial Interactions. The Sixth Annual University of Southern California Neuroscience Symposium, Los Angeles, November 6, 1998. (1998)
    Oxford, UK : Blackwell Publishing Ltd
    CNS drug reviews 4 (1998), S. 0 
    Details
    ISSN: 1527-3458
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1527-3458.1998.tb00078.x
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Disorganised and ‘excessive’ reinnervation of frog cardiac ganglia (1978)
    [s.l.] : Nature Publishing Group
    Nature 274 (1978), S. 286-288 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Table 1 Innervation pattern of neurones on the left branch of control and reinnervated cardiac ganglia in the frog No. of Animals Neurones % Of cells innervated by Left vagus Right vagus Both vagi Control ganglia Reinnervated ganglia 15 18 373 500 87.6±3.3%* 82.6±3.0* ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/274286a0
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Formation of the active zone at developing neuromuscular junctions in larval and adult bullfrogs (1985)
    Springer
    Journal of neurocytology 14 (1985), S. 487-512 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Development of the presynaptic active zone was studied at neuromuscular junctions with freeze-fracture electron microscopy in larval and adult bullfrogs. In rudimentary larval neuromuscular junctions, clusters of active zone particles were scattered over the P-face of the presynaptic membrane. Vesicle openings were observed at these terminals even though active zone particles lacked the mature pattern of two double rows. Gradually, active zone particles became organized into rows, but they were still randomly located and oriented. Once junctional folds were observed in replicas, developing active zones were located opposite to the folds, as in mature terminals. Multiple terminals occupying the same junctional gutters were also observed. At the end of metamorphosis, most active zones were still immature in appearance and had only grown to one third of their mature length. After metamorphosis, the number of active zone segments aligned at the same junctional fold increased. These discontinuous short active zones then elongated, joined together, and finally formed the mature active zones. Signs suggesting synapse elimination such as disorganization of active zones, absence of intramembrane particles in varicosities, and exposed muscle membranes with patches of acetylcholine receptor aggregates were observed. In some multiply innervated junctions, one terminal had mature active zones with vesicle openings, the other in the same gutter displayed disorganized active zones without vesicle openings, although both terminals showed similar sizes and distributions of background particles. This study suggests that developing active zones, as is the case for regenerating active zones in the adult, are functional before the mature organization is formed. The sequence of development of active zones is also similar to that of regeneration except for the random location and orientation of early active zones in tadpoles. The comparison between regeneration and development further indicates that the process of active zone formation is related to junctional folds and/or associated structures. It is also suggested that synapse elimination may involve degenerative changes in presynaptic membranes, although direct evidence remains to be provided.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01217757
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    A Schwann cell matrix component of neuromuscular junctions and peripheral nerves (1997)
    Springer
    Journal of neurocytology 26 (1997), S. 63-75 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Molecules localized to the synapse are potential contributors to processes unique to this specialized region, such as synapse formation and maintenance and synaptic transmission. We used an immunohistochemical strategy to uncover such molecules by generating antibodies that selectively stain synaptic regions and then using the antibodies to analyse their antigens. In this study, we utilized a monoclonal antibody, mAb 6D7, to identify and characterize an antigen concentrated at frog neuromuscular junctions and in peripheral nerves. In adult muscle, immunoelectron microscopy indicates that the antigen is located in the extracellular matrix around perisynaptic Schwann cells at the neuromuscular junction and in association with myelinated and nonmyelinated axons in peripheral nerves. The maintenance of the mAb 6D7 epitope is innervation-dependent but is muscle-independent; it disappears from the synaptic region within 2 weeks after denervation, but persists after muscle damage when the nerve is left intact. mAb 6D7 immunolabelling is also detected at the neuromuscular junction in developing tadpoles. Biochemical analyses of nerve extracts indicate that mAb 6D7 recognizes a glycoprotein of 127 kDa with both N- and O-linked carbohydrate moieties. Taken together, the results suggest that the antigen recognized by mAb 6D7 may be a novel component of the synaptic extracellular matrix overlying the terminal Schwann cell. The innervation-sensitivity of the epitope at the neuromuscular junction suggests a function in the interactions between nerves and Schwann cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018515526035
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 5
    Digitale Medien
    Digitale Medien
    A novel ω-conopeptide for the presynaptic localization of calcium channels at the mammalian neuromuscular junction (1995)
    Springer
    Journal of neurocytology 24 (1995), S. 15-27 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Voltage-sensitive Ca2+ channels are essential to transmitter release at the chemical synapse. To demonstrate the localization of voltage-sensitive Ca2+ channels in relation to the site of transmitter release, mouse neuromuscular junctions were double-labelled with α-bungarotoxin and a novel voltage-sensitive Ca2+ channel probe, SNX-260, a synthetic analog of ω-conopeptide MVIIC. Similar to ω-conopeptide MVIIC, biotinylated SNX-260 blocked nerve-stimulated transmitter release at the mouse neuromuscular junction. Fluorescently-tagged biotinylated SNX-260 labelled the nerve terminal which appeared thinner than and was outlined by acetylcholine receptor clusters as seen inen face view. This SNX-260 labelling was inhibited by preincubation with unconjugated SNX-260. Side-views of the neuromuscular junction indicated that the SNX-260 labelling was on the synaptic side facing the acetylcholine receptor rather than on the nonsynaptic side of the nerve terminal. This presynaptic binding was confirmed by the absence of SNX-260 labelling in denervated muscles following a nerve cut or disjunction after collagenase treatment. Confocal microscopy revealed spots of SNX-260 labelling that may correlate with active zones. The SNX-260 labelling pattern was not affected by preincubation with unconjugated SNX-111 (ω-conopeptide MVIIA), an N-type voltage-sensitive Ca2+ channel blocker. These findings suggest that SNX-260 is a novel probe for localizing non-N type voltage-sensitive Ca2+ channels and that these voltage-sensitive Ca2+ channels are localized near the transmitter release sites at the mammalian motor nerve terminal membrane. The results are consistent with the suggestion that non-N, probably P/Q type voltage-sensitive Ca2+ channels mediate evoked transmitter release at the mammalian neuromuscular junction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01370157
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 6
    Digitale Medien
    Digitale Medien
    A comparison of active zone structure in frog neuromuscular junctions from two fast muscles with different synaptic efficacy (1986)
    Springer
    Journal of neurocytology 15 (1986), S. 525-534 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary To search for ultrastructural correlates of differences in synaptic safety factor and neurotransmitter release, neuromuscular junctions from the cutaneous pectoris and cutaneous dorsi muscles of the grass frogRana pipiens were freeze fractured. Synaptic efficacy in these muscles was determined by the extent to which isometric twitch tension could be blocked by lowering [Ca2+] in the bathing solution. We found that junctions in the cutaneous pectoris were significantly more effective than those of the cutaneous dorsi. Morphometric analysis of 16 junctions from each type of muscle showed significant differences in some aspects of active zone structure. Cutaneous pectoris terminals had longer active zone segments and active zones spaced more closely together. This resulted in 20% more active zone length per unit terminal length in the cutaneous pectoris. Cutaneous dorsi terminals had active zones that were more often segmented into two or more sections at a single junctional fold. Mean active zone length per junctional fold and the number of active zone particles per micrometre of active zone length were not significantly different. As a result of the somewhat larger terminal width in the cutaneous dorsi, the percentage of terminal width occupied by active zone was greater in the cutaneous pectoris. As an attempt to indirectly estimate active zone spacing with the light microscope, we applied rhodamine-conjugated alpha bungarotoxin to neuromuscular junctions from the cutaneous pectoris and cutaneous dorsi. No significant difference in the spacing of fluorescently labelled acetylcholine receptor bands was found between the two types of junctions. Our results indicated that the greater active zone length per unit terminal length in the cutaneous pectoris was associated with its increased synaptic efficacy. In addition the continuity and particle organization of active zones may have contributed to the observed differences in synaptic safety factor at frog neuromuscular junctions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01611734
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 7
    Digitale Medien
    Digitale Medien
    A lectin, peanut agglutinin, as a probe for the extracellular matrix in living neuromuscular junctions (1987)
    Springer
    Journal of neurocytology 16 (1987), S. 567-576 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The extracellular matrix plays important roles in the differentiation of synapses. To identify molecules concentrated specifically in the synaptic extracellular matrix, fluorescently-labelled lectins were applied to neuromuscular junctions. A lectin, peanut agglutinin (PNA), stains the neuromuscular region selectively and irreversibly (up to at least 3 weeksin situ), outlining the periphery of the nerve terminal arborization in the frog. Snake neuromuscular junctions also stain intensely with fluorescent PNA, while mouse diaphragm staining is faint. At the electron microscopic level, the reaction products of horseradish peroxidase-conjugated PNA are found primarily in the extracellular matrix flanking Schwann cells in the frog endplate regions. Fluorescently labelled PNA does not affect synaptic potentials and can serve as a simple stain for correlating functional studies of living neuromuscular junctions. Moreover, it can be combined with a presynaptic dye to observe nerve terminals and synaptic extracellular matrix in the same junctionsin situ. This report reveals the existence of synapse-specific carbohydrates associated with Schwann cell extracellular matrix in the frog neuromuscular junction. The specific binding and its physiological compatibility make PNA a useful probe for further investigation of synaptic differentiation, plasticity and maintenance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01668509
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 8
    Digitale Medien
    Digitale Medien
    Disruption of active zones in frog neuromuscular junctions following treatment with proteolytic enzymes (1988)
    Springer
    Journal of neurocytology 17 (1988), S. 63-71 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Frog neuromuscular junctions treated with proteolytic enzymes to remove the basal lamina were studied with freeze-fracture techniques in order to examine the influence of the basal lamina in the maintenance of active zone ultrastructure. The active zone is believed to be the site of transmitter release and has a unique membrane organization and location in the neuromuscular junction. After removal of the basal lamina by successive treatment of 0.01% collagenase and 0.1% protease for 1 h each, active zone disruption was observed. Some active zones became segmented, and some were also randomly located and oriented, but they still had normal double-row particle organization. Others contained only clusters of large intramembrane particles. These disorganized active zones were still functional as indicated by the presence of vesicle openings. Some enzyme-treated junctions were also exposed to the membrane cholesterol probe, filipin, to examine the expression of membrane lipid heterogeneity in disrupted active zones. As in normal active zones, filipin—sterol complexes were absent. The densities of background particles in the presynaptic membranes and of large particles thought to be acetylcholine receptors were not significantly altered by the enzyme treatment. Although a direct effect of the enzymes on active zone ultrastructure can not be totally excluded, the present work is consistent with a maintenance role of the basal lamina in active zone organization and location.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01735378
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 9
    Digitale Medien
    Digitale Medien
    Absence of sterol-specific complexes at active zones of degenerating and regenerating frog neuromuscular junctions (1986)
    Springer
    Journal of neurocytology 15 (1986), S. 231-240 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Freeze-fracture combined with filipin treatment has been used as a cytochemical probe for membrane cholesterol. As previously shown at the frog neuromuscular junction, distinctive sterol-specific complexes were formed on the presynaptic membrane after filipin treatment, except at active zones. The absence of sterol-specific complexes from active zones was confirmed using two other cytochemical agents — digitonin and saponin. We also studied the maintenance and differentiation of the presynaptic membrane heterogeneity revealed by membrane cholesterol probes at degenerating and regenerating neuromuscular junctions. During degeneration, active zones in frog nerve terminals were disorganized, but still lacked sterol-specific complexes. After engulfing the degenerating nerve terminals, Schwann cells occupied the synaptic gutters and displayed a uniform distribution of sterol-specific complexes. Schwann cell ridges opposite the postjunctional folds also had prominent sterol-specific complexes in regions formerly occupied by active zones. By 2 weeks after nerve crush, nerve terminals reinvaded the endplate region and active zones began to regenerate. While the intramembrane particles of the early regenerating active zones were not arranged in the normal double-rowed organization, filipin-sterol complexes were nevertheless excluded from these primitive active zones. Areas of nerve terminal membrane opposite to junctional folds but lacking active zones were covered with filipin-sterol complexes. These results show that the normal double-rowed organization is not required for the expression of the membrane heterogeneity associated with the active zone. In addition, the absence of sterol-specific complexes is closely associated with the active zone particles and not simply the membrane regions opposite to the postjunctional folds. The membrane heterogeneity does not seem to be directly linked with the functional state of the active zone since it is still associated with degenerating active zones after transmission failure has occurred.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01611659
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 10
    Digitale Medien
    Digitale Medien
    Perisynaptic Schwann cells at neuromuscular junctions revealed by a novel monoclonal antibody (1998)
    Springer
    Journal of neurocytology 27 (1998), S. 667-681 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract This study aimed to generate a probe for perisynaptic Schwann cells (PSCs) to investigate the emerging role of these synapse-associated glial cells in the formation and maintenance of the neuromuscular junction (NMJ). We have obtained a novel monoclonal antibody, 2A12, which labels the external surface of PSC membranes at the frog NMJ. The antibody reveals PSC fine processes or “fingers” that are interposed between nerve terminal and muscle membrane, interdigitating with bands of acetylcholine receptors. This antibody also labels PSCs at the avian neuromuscular junction and recognizes a 200 kDa protein in Torpedo electric organs. In frog muscles, axotomy induces sprouting of PSC processes beyond clusters of acetylcholine receptors and acetylcholinesterase at denervated junctional branches. PSC branches often extend across several muscle fibers. At some junctions, PSC sprouts join the tips of neighboring branches. The average length of PSC sprouts is approximately 156 µ at 3-week denervated NMJs. PSC sprouting is accompanied by a significant increase in the number of Schwann cell bodies per NMJ. Following nerve regeneration, nerve terminals reinnervate the junction along the PSC processes. In vivo observations of normal frog muscles also show PSC processes longer than nerve terminals at some junctional branches. The results suggest that nerve injury induces profuse PSC sprouting that may play a role in guiding nerve terminal regeneration at frog NMJs. In addition, antibody 2A12 reveals the fine morphology of PSCs in relation to other synaptic elements and is a useful probe in elucidating the function of these synapse-associated glial cells in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006916232627
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...