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  • 1
    Electronic Resource
    Electronic Resource
    Co-ordination between membrane oriC sequestration factors and a chromosome partitioning protein, TolC (MukA) (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 22 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: oriC DNA in the hemimethylated (but not in the fully methylated) state reacts with an Escherichia coli K-12 outer membrane preparation. This reaction is drastically reduced when the membrane preparation of a seqA null mutant is used. An in vitro reconstitution of the activity was undertaken by adding a partially purified SeqA protein to a seqA mutant membrane without success. A possible reason for this failure might be a profound modification of the outer membrane of the seqA mutant (as revealed by the fact that membrane from the mutant sediments more slowly than that from the wild type during ultracentrifugation). There is also a reduction in the content of OmpF protein. Moreover, one of the minor outer membrane proteins involved in partitioning of newly synthesized chromosomes, the TolC (MukA) protein, was also found to be downregulated in the seqA mutant. This is also true of the hobH mutant grown in a high-osmolarity medium. Mutants of both seqA and hobH stop dividing after hyperosmotic shock, forming filaments (as observed in dam mutants).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.00106.x
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  • 2
    Electronic Resource
    Electronic Resource
    Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 31 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The lacZ–hobH fusion clone, containing an Escherichia coli DNA segment located at 92 min on the chromosomal map, was screened as a producer of E. coli oriC hemi-methylated binding activity. We have purified the protein encoded by this locus to near homogeneity. The protein corresponds to the monomeric form of a non-specific acid phosphatase (NAP) whose gene has been designated aphA. oriC DNA footprinting experiments showed protection of hemi-methylated probe by partially purified NAP, but not by purified preparations. Yet, gel retardation experiments with an oriC oligonucleotide demonstrated DNA binding activity of purified NAP in the presence of Mg2+. This experiment also showed an increased affinity of the protein for the hemi-methylated probe compared with the fully or unmethylated form. Indirect immunofluorescence microscopy revealed the existence of discrete NAP foci at mid-cell in cells with two nucleoids, but at cell poles in those with one nucleoid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01156.x
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  • 3
    Electronic Resource
    Electronic Resource
    The initiation mess? (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: This review concerns the mechanisms which control initiation of chromosome replication in enterobacteria with respect to cell growth. Initiation control is commonly separated into positive and negative regulatory mechanisms. Four main points are advanced concerning these different aspects of initiation control. (i) The average concentration of the initiator protein DnaA is proportional to the origin concentration, i.e. the origin per cell mass ratio and, thus, inversely proportional to the very often used term of the ‘initiation mass’. (ii) The time of initiation of chromosome replication in the cell cycle is set by DnaA protein accumulating to a threshold level, which in concert with a number of other factors allows for a co-operative formation of the initiation complex. (iii) The time of initiation is not determined by the interaction with these other factors or by the transient interaction between newly replicated origins (oriC ) and the cell surface. (iv) The aberrant initiation phenotype observed in various mutants, including dnaA (ts) mutants, might be due to a defective pre-initiation DnaA–oriC interaction or it might be due to a defect in the protection of newly initiated origins from reinitiation. Many of these points are discussed and evaluated in view of recent developments concerning the regulation of chromosome replication in Escherichia coli
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.432956.x
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  • 4
    Electronic Resource
    Electronic Resource
    Appearance of New Deficient Characters in Streptomycin-resistant Mutants of Bacillus subtilis (1960)
    [s.l.] : Nature Publishing Group
    Nature 187 (1960), S. 168-169 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The first experiment was carried out with the met, his,str-s strain. About 5 X 108 cells harvested from a culture in nutrient broth were plated on nutrient agar and a synthetic medium (Gray and Tatum's medium plus methionine and histidine). Each medium contained streptomycin in the amounts shown in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/187168a0
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  • 5
    Electronic Resource
    Electronic Resource
    Temperature Sensitive Mutant of the DNA Replication System in Escherichia coli (1970)
    [s.l.] : Nature Publishing Group
    Nature 228 (1970), S. 1157-1160 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The true DNA replicase system of E. coli may have been identified by assaying DNA synthesis in toluenized bacteria of a temperature sensitive DNA replication ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2281157a0
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  • 6
    Electronic Resource
    Electronic Resource
    Potassium ions and changes in bacterial DNA supercoiling under osmotic stress (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 99 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK756 behaves like the K+ transport mutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05559.x
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  • 7
    Electronic Resource
    Electronic Resource
    Impairment of nucleoid segregation and cell division at high osmolarity in a strain of Escherichia coli overproducing the chaperone DnaK (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 113 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Escherichia coli strain WM1390, which overproduces 20-fold the chaperone protein DnaK, was able to grow exponentially without apparent abnormality in 300 mosM medium. In contrast, it showed both aberrant nucleoid segregation and strong inhibition of septation when shifted to high osmolarity. These impairments could not be accounted for by a bacteriocidal effect of the high DnaK content. Rather, the DnaK content appeared to promote faster growth than that of the parent C600, at least at high osmolarity in the presence of the osmoprotectant glycine betaine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06494.x
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  • 8
    Electronic Resource
    Electronic Resource
    ATP is required for K+ active transport in the archaebacterium Haloferax volcanii (1989)
    Springer
    Archives of microbiology 151 (1989), S. 530-536 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; K+ transport ; Haloferax volcanii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Archaebacterium Haloferax volcanii concentrates K+ up to 3.6 M. This creates a very large K+ ion gradient of between 500- to 1,000-fold across the cell membrane. H. volcanii cells can be partially depleted of their internal K+ but the residual K+ concentration cannot be lowered below 1.5 M. In these conditions, the cells retain the ability to take up potassium from the medium and to restore a high internal K+ concentration (3 to 3.2 M) via an energy dependent, active transport mechanism with a K m of between 1 to 2 mM. The driving force for K+ transport has been explored. Internal K+ concentration is not in equilibrium with ΔΨm suggesting that K+ transport cannot be accounted for by a passive uniport process. A requirement for ATP has been found. Indeed, the depletion of the ATP pool by arsenate or the inhibition of ATP synthesis by N,N′-dicyclohexylcarbodiimide inhibits by 100% K+ transport even though membrane potential ΔΨm is maintained under these conditions. By contrast, the necessity of a ΔΨm for K+ accumulation has not yet been clearly demonstrated. K+ transport in H. volcanii can be compared with K+ transport via the Trk system in Escherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454870
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic interaction observed in Bacillus subtilis (1959)
    Springer
    Molecular genetics and genomics 90 (1959), S. 347-355 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Some evidence was obtained that genetic interaction occurs inBacillus subtilis K. A mixed inoculation of two doubly auxotrophic mutants onto approriate media yielded tiny colonies which seemed to be initiated by heterocaryons or heterozygotes. The tiny colonies contained not only a recombinant type which acquired two characters from one or another parent, but also some abnormal types having new characters which were not recognized in either parent. The phenomenon is similar to the genetic interaction found inStreptomyces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00888809
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  • 10
    Electronic Resource
    Electronic Resource
    Recognition sites for a membrane-derived DNA binding protein preparation in the E. coli replication origin (1983)
    Springer
    Molecular genetics and genomics 191 (1983), S. 460-465 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The DNA binding protein B' preparation, isolated from the membrane of E. coli, recognizes two sites, one of which is locatd in the minimum oriC (35–270 bp) and the other between base pairs 417 and 488. Recognition is only possible when restriction fragments containing these sites are in single-stranded state. At the first site the strand reading 3′OH-5′P in the direction of the E. coli genetic map is recognized, at the second site the 5′P-3′OH strand.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425763
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