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  • 1
    Electronic Resource
    Electronic Resource
    Effects of spermidine on the synthesis of α-amylase in cotyledons of mung bean seedlings (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: When cotyledons of mung bean [Vigna radiata (L.) Wilczek] were treated with spermidine (3 mM) during the first 6 h of imbibition, the development of α-amylase activity in cotyledons during the following 3 days was severely inhibited (75%) This inhibition was due to a slower accumulation of α-amylase protein, which in turn resulted from an inhibition of α-amylase synthesis. The rise in the level of α-amylase mRNA in cotyledons was also inhibited by spermidine treatment. However, the degree of inhibition of mRNA accumulation (40%) was not so marked as that of the activity of α-amylase synthesis (80%). These results are discussed in relation to the mode of action of spermidine on α-amylase expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02131.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic analysis of fertility restoration and accumulation of ORF125 mitochondrial protein in the kosena radish (Raphanus sativus cv. Kosena) and a Brassica napus restorer line (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 949-955 
    Details
    ISSN: 1432-2242
    Keywords: Key words. Fertility Restoration (Rf) ; Cytoplasmic male sterility (CMS) ; Raphanus sativus L. ; Brassica napus ; Mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genetics of fertility restoration (Rf) of kosena radish CMS has been characterized. The kosena CMS-Rf system is genetically the same as that of the ogura CMS-Rf system. Two dominant genes that act complementary to the restoration of fertility control fertility restoration in kosena CMS. One allele (Rf1) is associated with accumulation of the CMS-associated protein, ORF125. The interaction of Rf1 and another allele (Rf2) was essential for the restoration of fertility in radish, whereas Rf1 alone was sufficient for the complete restoration of fertility in the B. napus kosena CMS cybrid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051375
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of α-amylase in response to wounding in mung bean (1995)
    Springer
    Planta 195 (1995), S. 530-534 
    Details
    ISSN: 1432-2048
    Keywords: α-Amylase ; mRNA (α-amylase) ; Vigna ; Wounding response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of α-amylase (EC 3.2.1.1) in mung bean (Vigna radiata (L.) Wilczek) cotyledons increased markedly in response to wounding. The changes in enzyme activity were in parallel with those in enzyme content. The level of α-amylase mRNA also notably increased in wounded cotyledons and attained its maximum level during the period between 1 and 2 d after wounding. The level of mRNA for phenylalanine ammonia-lyase, which is one of the well-characterized stress-inducible proteins, also increased after wounding, but the increase in mRNA level was faster than that of α-amylase mRNA. On the other hand, the content of mRNA for actin, a ‘housekeeping’ protein, was almost the same in wounded and unwounded cotyledons. The increase in α-amylase mRNA level in wounded cotyledons was severely inhibited by α-amanitin and cordycepin. α-Amylase expression in the first leaves of mung-bean seedlings was also induced by wounding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195711
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  • 4
    Electronic Resource
    Electronic Resource
    Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orf138 (1999)
    Springer
    Plant molecular biology 39 (1999), S. 183-188 
    Details
    ISSN: 1573-5028
    Keywords: cytoplasmic male sterility (CMS) ; mitochondrial DNA ; CMS-associated gene ; radish ; fertility restoration gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A CMS-associated gene, orf125, present in the Japanese radish cultivar Kosena, has a sequence homologous to that of the ogura CMS-associated gene, orf138, except for two amino acid substitutions and a 39 bp deletion in the orf138 coding region. In Kosena radish, orf125 is linked with orfB, whereas the orf125 locus differs in a Brassica napus CMS cybrid derived from protoplast fusion between Kosena radish and B. napus. A novel mtDNA sequence is present in the 3′-flanking region of orf125 in the B. napus kosena CMS cybrid. The orf125 is expressed both in the radish and the B. napus kosena CMS cybrid. Its accumulation is strongly associated with the CMS phenotype in B. napus. Fertility restoration was accompanied by a decrease in the amount of ORF125 in B. napus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006198611371
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