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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 22 (1997), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Methanogenesis per ciliate protozoan and most probable numbers (MPN) of methanogens per ciliate were measured and observed to be affected by feeding. The close similarity observed between the changes in apparent methane production and the changes in the number of methanogens per ciliate indicated that apparent methane production by ciliates depended primarily on the number of methanogens associated with them. Large changes in the MPN of methanogens associated with ciliates occurred rapidly. It is likely that these changes reflect the number of methanogens that associated extracellularly with the ciliates. Methanogens associated with ciliates may have differed from the free living methanogens in halophilism and their resistance to ammonium ions.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1436-2813
    Keywords: hand replantation ; arterio-venous fistula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Replantation of a completely severed finger, hand or arm is now performed with wide success. We now report an extremely rare complication of this replantation, an arterio-venous (A-V) shunt formation. Pathogenesis of the new A-V fistual formation is discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 39 (1999), S. 123-128 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The phylogeny of methanogenic archaea associated with ciliate protozoa in a sheep rumen was investigated. Ruminal ciliate protozoa were exhaustively washed and mixtures of genomic DNA extracted. Archaea-specific nested PCR amplification was conducted with the ciliate genomic mixture. The resultant small subunit (16S) ribosomal RNA gene (ssu rDNA) was cloned into Escherichia coli JM 109. Many methanogens were still observed on and/or in ciliate cells by fluorescent microscopy even after exhaustive washing with buffer. Partial sequences of ssu rDNA close to Methanobrevibacter smithii were dominant in the retrieved sequences. RFLP analyses on the retrieved sequences revealed the absence of Methanobrevibacter ruminantium in the protozoal preparation. The association of Methanobrevibacter spp. with ruminal ciliate protozoa was demonstrated by the isolation of archaeal ssu rDNA phylogenetically close to that of M. smithii.
    Type of Medium: Electronic Resource
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