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  • 1
    Electronic Resource
    Electronic Resource
    TRIC channels are essential for Ca 2+ handling in intracellular stores (2007)
    [s.l.] : Nature Publishing Group
    Nature 448 (2007), S. 78-82 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cell signalling requires efficient Ca2+ mobilization from intracellular stores through Ca2+ release channels, as well as predicted counter-movement of ions across the sarcoplasmic/endoplasmic reticulum membrane to balance the transient negative potential generated ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05928
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of calcium in the chick yolk sac membrane endodermal cells as revealed by cytochemistry and X-ray microanalysis (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 607-614 
    Details
    ISSN: 1432-0568
    Keywords: Chick embryo ; Yolk sac membrane ; Calcium transport ; Cytochemistry ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The yolk sac membrane (YSM) of the chick embryo transports calcium from the yolk into the embryonic circulation during the first half of development, but the intracellular pathway of calcium transport is poorly understood. In the present study, the ultrastructural localization of calcium was investigated in cells of the YSM of 9-day chick embryos. X-ray microanalysis as well as cytochemical techniques performed on yolk sac membrane cells treated with potassium oxalate, potassium ferricyanide and potassium antimonate demonstrated accumulation of calcium in yolk granules, digested yolk products, electron-dense bodies (EDBs; 100–400 nm diameter) and electron-dense granules (EDGs; 30–50 nm diameter). When strontium ions were injected into the yolk, they were incorporated into the endodermal cells and sequestered specifically in EDGs. From these results, we propose that calcium enters the endodermal cells by endocytosis of calcium-containing yolk granules, as well as through calcium channels in the apical cell membrane. In the cytoplasm, digested yolk products, EDBs, and EDGs act as sites of sequestration and accumulation of calcium. Extrusion of intracellular calcium into the extracellular space and embryonic circulation is accomplished by exocytosis of calcium-containing material and via an ion pump in the basal cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214440
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  • 3
    Electronic Resource
    Electronic Resource
    Calcium-containing, smooth-surfaced endoplasmic reticulum and vacuoles in cells of the blastopore-forming region during gastrulation of the newt, Cynops pyrrhogaster (1995)
    Springer
    Anatomy and embryology 191 (1995), S. 369-376 
    Details
    ISSN: 1432-0568
    Keywords: Amphibian gastrulation ; Blastopore formation ; Smooth-surfaced endoplasmic reticulum Calcium ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ultrastructural changes in cells of the blastopore-forming region (BFR) were examined during gastrulation of the newt, Cynops pyrrhogaster. Smooth-surfaced endoplasmic reticulum (sER), tubular in shape and with two types of vacuole, empty vacuoles and multilamellar vacuoles, appeared in cells of the BFR at the beginning of formation of the blastopore. The extent of the tubular sER increased during formation of the blastopore. With the deepening of the blastoporal groove, the tubular sER and multilamellar vacuoles disappeared in cells of the BFR. The empty vacuoles increased in number and appeared throughout the cytoplasm of the cells in the BFR and the marginal zone as formation of the archenteron progressed. Many large empty vacuoles were closely associated with deformed lipid droplets. Cytochemistry and X-ray microanalysis revealed the accumulation of calcium in the tubular sER and in some of the empty vacuoles. These results suggest important roles for the calcium-containing tubular sER in the regulation of the intracellular concentration of calcium during formation of the blastopore, as well as a role for empty vacuoles in the dissolution and consumption of lipid droplets during early gastrulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00534690
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of calcium in the chick chorioallantoic membrane as revealed by cytochemistry and X-ray microanalysis (1992)
    Springer
    Anatomy and embryology 186 (1992), S. 529-535 
    Details
    ISSN: 1432-0568
    Keywords: Chick ; Chorioallantoic membrane ; Calcium ; Cytochemistry ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The chorioallantoic membrane (CAM) of the chick embryo actively transports calcium from the egg shell into the embryonic circulation. To investigate the intracellular pathway of calcium transport across the CAM, ultrastructural localization of intracellular calcium in cells of the chorionic ectoderm (CE) was determined using cytochemical methods and X-ray microanalysis. Treatment of the CE with potassium oxalate, potassium ferricyanide or potassium pyroantimonate revealed large numbers of electron-dense granules (EDGs) in the ectodermal cells. These measure 30–40 nm in diameter, and are not membrane-bound. These granules were seen in all three cell types of the CE. The presence of calcium in the EDG was directly confirmed by X-ray microanalysis. When strontium or barium ions were applied to the shell membrane side of the CAM, the cells of the CE incorporated these divalent cations and sequestered them in granules (25–40 nm in diameter) in cytoplasm and mitochondria. This study indicates that calcium enters the CE cells by means other than endocytosis, as the EDGs are not membrane-bound, that all three types of the CE cells appear to function in transport of calcium from shell to embryo during embryogenesis, and that the EDG plays important roles in intracellular accumulation of calcium during the process of calcium transport across the chorioallantoic membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186975
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  • 5
    Electronic Resource
    Electronic Resource
    Calcium-containing vacuolated mitochondria during early heart development in chick embryos as demonstrated by cytochemistry and X-ray microanalysis (1994)
    Springer
    Anatomy and embryology 189 (1994), S. 441-446 
    Details
    ISSN: 1432-0568
    Keywords: Chick heart development ; Mitochondria ; Calcium ; Cytochemistry ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of mitochondria in the developing heart was examined in chick embryos from 2 to 7 days after fertilization. Vacuolated mitochondria were observed in the heart muscle cells of embryos at all stages examined. The number of vacuolated mitochondria as a percentage of total mitochondria in muscle cells was high in embryos at 3 and 4 days and was much higher in the ventricular cells than in the atrial cells. Examination by cytochemistry and X-ray microanalysis revealed the accumulation of calcium in the vacuoles of mitochondria. These results suggest important roles for vacuolated mitochondria in the regulation of the intracellular concentration of calcium during the early development of the chick heart.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185439
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  • 6
    Electronic Resource
    Electronic Resource
    A morphological study on regulation of hyaline bleb formation in early embryonic cells of Cynops pyrrhogaster (1991)
    Springer
    Cell & tissue research 263 (1991), S. 337-344 
    Details
    ISSN: 1432-0878
    Keywords: Early development ; Cleavage cycle ; Hyaline bleb ; Submembranous cortical layer ; Electric pulses ; Cynops pyrrhogaster (Urodela)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Presumptive ectodermal cells isolated from the animal pole region of Cynops pyrrhogaster embryos showed active formation of hyaline blebs after the eleventh cleavage. The role of the submembranous cortical layer (SCL) in hyaline bleb formation was investigated in relation to the number of cleavages. Rhodamine-phalloidin staining and transmission electron-microscopy showed structural changes in the SCL after the eleventh cleavage. Partial breakdown of the SCL by electric pulses and deformation of the SCL by treatment with cycloheximide and nocodazole induced hyaline bleb formation before the eleventh cleavage. These results suggest that the SCL plays an important role in hyaline bleb formation after the eleventh cleavage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318775
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