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  • 1
    Electronic Resource
    Electronic Resource
    Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe (2000)
    Springer
    Biochemical genetics 38 (2000), S. 1-11 
    Details
    ISSN: 1573-4927
    Keywords: mouse testis ; poly(A)binding protein ; meiosis ; Schizosaccharomyces pombe.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A cDNA clone from a mouse testis cDNA library was isolated by the transcomplementation method using a Schizosaccharomyces pombe meiotic mutant (sme2) that is defective in meiosis I. The cDNA clone isolated has an open reading frame encoding 302 amino acids constituting a protein with a strong similarity to mouse poly(A) binding protein II (mPABII) and bovine poly(A) binding protein II (PABII). PABII is known to bind to the growing poly(A) tail and stimulates poly(A) polymerase, which catalyzes the polymerization of the mRNA poly(A) tail. Northern blot analysis of the cDNA clone identified as mPABII revealed a single transcript of 1.2 kb. This was detectable exclusively in adult testis. Immunohistochemical analysis using a polyclonal antibody demonstrated that mPABII protein was expressed in the nucleus at specific stages from late pachytene spermatocytes to round spermatids. Genetic mapping showed the Pabp3 gene encoding mPABII to be located near position 19.5 on mouse chromosome 14. These results suggest that mPABII might be involved in specific spermatogenetic cell differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1001876119130
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  • 2
    Electronic Resource
    Electronic Resource
    Jumbled spine and ribs (Jsr): a new mutation on mouse Chromosome 5 (1999)
    Springer
    Mammalian genome 10 (1999), S. 213-217 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Jumbled spine and ribs (Jsr) is an autosomal dominant mutation that results in malformation of the axial skeleton. The vertebrae of mutant mice (Jsr/+) are all shorter than those of normal mice (+/+) in the inbred line and show various abnormalities. In addition, several ribs are fused at their proximal region because of fusion of thoracic vertebrae. In this study, we localized the Jsr mutation on distal Chromosome (Chr) 5 and constructed a high-resolution map. Chromosomal mapping was performed with an inter-subspecific backcross of (CKH-Jsr/+× MOG) F1 carrying the Jsr allele and CKH-+/+. The predicted gene order around Jsr was determined to be cen–(Epo, Pdgfa, D5Mit31, D5Mit374)–(Jsr, Nfe2u, D5Mit99, D5Mit247, D5Mit284, D5Mit292, D5Mit327)–D5Mit328–tel. Subsequently, high-resolution mapping concluded the Jsr localization to be cen–Nfe2u–1.0cM–Jsr–0.2cM–D5Mit247,292–tel. Jsr/Jsr homozygotes are alive, as the mutation is not lethal. Based on histological analysis of mutant embryos, Jsr is hypothesized to be caused by abnormal development of primordial cells in the axial skeleton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900975
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemical studies of renin-containing cells in the developing sheep kidney (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 239 (1994), S. 191-197 
    Details
    ISSN: 0003-276X
    Keywords: Development ; Immunohistochemistry ; Renin-containing cells ; Sheep ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: Renin-containing (RC) cells in small ruminant kidneys have been known to be widely distributed along the blood vessels. In the present study, RC cells in developing sheep kidneys were studied to investigate not only the appearance but distribution with the potential physiological significance using immunohistochemical and histophanimetrical techniques.Methods: Seven fetal, 12 newborn, and 3 adult metanephric kidneys were used and immunostained by anti-renin antiserum. In the histoplanimetrical analysis, the numerical values of RC cells existing at the walls of 3 major arterial types in the kidneys were calculated.Results: At day 44 of gestation, RC cells were already demonstrated in the walls of renal, interlobar, and afferent vessels, located in the deep cortex and the medulla. In intermediate gestational periods, RC cells were detected throughout the intrarenal arterial trees. In late gestational periods, RC cells expressed in the walls of interlobar/arcuate and interlobular arteries tended to decrease or disappear gradually, while they were distributed predominantly in the afferent glomerular vessels. In newborn lambs, especially days 1 to 3 after birth, increased numbers of RC cells were demonstrated throughout the arterial trees in the kidneys. In older lambs, RC cells located in the interlobar/arcuate arteries and the proximal region of the interlobular arteries decreased in number and gradually disappeared. Some RC cells were still distributed in the distal portion of the interlobular artery even in the adult sheep.Conclusions: These results suggest that the wide distribution of RC cells in sheep kidney is formed in perinatal life, and that the neuronal regulation is associated with the maintenance of this distribution. © 1994 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092390210
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of renin in coagulating glands is regulated by testosterone (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 241 (1995), S. 451-460 
    Details
    ISSN: 0003-276X
    Keywords: Castration ; Coagulating gland ; Local renin-angiotensin ; Mouse ; Testosterone ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: The presence of extrarenal or local renin-angiotensin system (RAS) has been noted in several tissues, although its functions have not yet been clarified. Renin from the coagulating gland (CG) is the most recently discovered local RAS and is a significant subject for investigation because large amounts of both mRNA and proteins are detected in this organ. Recently, it has been reported that testosterone influences renin synthesis in several extrarenal tissues, although it has no effect on intrarenal renin. Therefore, it is possible that CG renin is also regulated by testosterone.Methods: Forty-four male C57BL/6 mice, aged 3 wk to 6 mo, were used in studies on the ontogeny and androgen regulation of the RAS in the CG. The tissues were fixed with Bouin's solution and paraffin sections were stained with immunohistochemical methods using antirenin antiserum. In each immunostained section, the relative number of renin-containing cells in terminal portions of the CG were counted.Results: Immunoreactivity for renin was first detected at 6 wk after birth. After that time, the number of renin-containing cells gradually increased throughout the experiment. In adults, several patterns of renin immunoreactivity were demonstrated in almost all epithelial cells of CGs, specifically; (1) basolateral granular reaction, (2) diffuse immunoreactivity throughout the cytoplasm, and (3) restricted nuclear reaction. Excretory products of some terminal lumina were also found to be positive for renin. At 10 days after castration, renin-containing cells in ductal termini were decreased and remained at low levels until at 4 wk after castration. After testosterone injection, numerical values of renin-containing cells were high at 1 wk and then decreased at 2-3 wk.Conclusion: It is suggested that CG renin of the mouse is expressed together with sexual maturation during development and that it depends on the testis, possibly the male sex hormone. © 1995 Wiley-Liss, Inc.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092410403
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution of epithelioid cells in the wall of the chicken aorta and their functional role as chemoreceptors (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 242 (1995), S. 302-309 
    Details
    ISSN: 0003-276X
    Keywords: Aorta ; Chicken ; Chemoreceptor ; Epithelioid cells ; Wholemount immunohistochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: The ultrastructural characteristics of epithelioid cells in the wall of the chicken aorta have been studied by several investigators. Their characteristics were homologous to those of carotid body type I cells and are considered to be one of the peripheral chemoreceptors. However, there are few descriptions about their location, distribution, and how they react to chemical signals, nor have there been many reports about the localization of bioactive substances in the epithelioid cells. Therefore we designed this investigation to address these problems.Methods: Wholemount immunohistochemistry using antiserotonin antiserum, scanning (SEM), and transmission (TEM) electron microscopy were used to observe the epithelioid cells and the lumen of the chicken aorta. The localizations of bioactive substances in the epithelioid cells were immunohistochemically investigated using 11 antisera.Results: Epithelioid cells were dispersed in the wall of the aorta, forming a band ∼1 mm in width, located 10 mm proximal to the confluence of the right and left ligamenta arteriosa. Serotonin, chromogranin, and neuron specific enolase immunoreactivities were detected in the epithelioid cells. SEM observations clearly demonstrated intraendothelial fenestrations, 1-3 μm in diameter, on the endothelial surface of the region of the band of epithelioid cells. TEM observations revealed that these fenestrations corresponded to endothelial gaps, directly beneath which epithelioid cells were sometimes located.Conclusions: The epithelioid cells are in direct access to the aortic lumen through endothelial fenestrations. Thus they may be able to perceive chemical signals from arterial blood directly. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092420303
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological evidence of exocrine function in coagulating gland renin of mouse strain C57BL/6 (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 243 (1995), S. 200-207 
    Details
    ISSN: 0003-276X
    Keywords: Coagulating gland ; Exocrine ; Immunoelectron microscopy ; Mouse ; Renin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: Renin is classically secreted from juxtaglomerular cells of the kidney by endocrine or paracrine mechanisms. In a previous report, intense renin immunoreactivity was observed in the coagulating gland (CG), a new source for renin in mice. In the present study, immunoelectron microscopical analysis for renin was carried out to clarify the secretory site of CGs.Methods: Five adult male C57BL/6 mice were used in this study. The CGs were fixed with an ice-cold 2% glutaraldehyde and 2% paraformaldehyde mixture and embedded in Lowicryl K4M. Ultrathin sections were treated with antimouse renin antiserum and colloidal gold (15 nm)-labeled protein A complex.Results: In immunoelectron microscopical observation, renin was first detected at the Golgi vacuoles just budding from the lamellae, although it was not demonstrated in all Golgi vacuoles. In the production series of exocrine granules, renin immunoreactivity was observed in some granules that were distributed in the supranuclear region. Both renin-positive and negative exocrine granules were secreted from the apical cell membrane by exocytosis. The lysosomal granules also showed stronger renin immunoreactivity and contained homogeneous or heterogeneous materials. In the supranuclear region, it was observed that exocrine granules were fused with irregular lysosomal granules. At the apical region, such lysosomal granules were closely associated with cell membrane. At the basolateral region, immunoreactivity for renin was localized in electron dense granules.Conclusions: These results suggest that part of the renin in the CGs is released by exocrine secretion into the genital tract. © 1995 Wiley-Liss, Inc.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092430207
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  • 7
    Electronic Resource
    Electronic Resource
    Morphological and northern blot analysis of juxtaglomerular cells in experimental hydronephrotic mice (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 232 (1992), S. 393-400 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The purpose of this study is to demonstrate the developmental changes of the experimental hydronephrotic kidney using immunohistochemical, histoplanimetrical, and Northern blot techniques. At 1 month after ligation of the ureter, a large number of renin-positive cells were detected immunohistochemically even at a dilution of 1:10,000 in this hydronephrotic kidney; however, there were few renin-positive cells in the non-ligated side. At 6 months after ligation, no difference in reactivity for renin between ligated and non-ligated kidneys was demonstrated. In the morphometrical analysis of the renin-positive region, the numerical value of the ligated side was already increased at 2 weeks, reached the highest value at 1 month, and then decreased gradually to almost the same value as the control kidney by the end of the experiment. On the other hand, the value of the non-ligated side decreased immediately after the unilateral ligation, increased later, and finally reached almost the same value as the control kidney. In the Northern blot analysis, the activity of renin mRNA in the ligated side at 1 month after ligation was markedly higher than that in the non-ligated side. However, the difference between the ligated and the non-ligated sides was not demonstrated at 6 months and the value came to be almost the same as in the nonoperated kidney.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092320309
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