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  • 1
    Electronic Resource
    Electronic Resource
    Transgenic rice plants: Characterization of two generations of seed progeny (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Transgenic rice plants have been regenerated from kanamycin-resistant callus of Oryza sativa (cv. Taipei 309) derived from protoplasts electroporated with pCaMVNEO carrying the neomycin phosphotransferase II (nptII) gene. Of 6 randomly selected plants, all contained the nptll gene, but only 2 plants expressed NPTII activity. The transgenic plants were significantly shorter, produced fewer tillers, took longer to flower and had reduced fertility compared to non-transformed protoplastderived plants. Fifty-six seeds collected from one transgenic plant expressing NPTII activity germinated on medium containing kanamycin sulphate to give 16 green, first seed generation (R1) plants. The latter could be divided into 3 groups: (i) Plants which set seed, had normal floret morphology and produced a total of 76 seeds; (ii) Plants which flowered, but which failed to set seed; (iii) Plants which failed to flower, were shorter and had significantly fewer tillers than plants of groups (i) and (ii). The nptII gene was present in all transgenic R1 plants, but only 8 plants expressed the gene. Phenotypic characteristics, observed in transgenic R1 plants were also seen in the transforned R2 plants. These included reduced stature, a longer vegetative phase and reduced fertility compared to non-transformed plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04748.x
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  • 2
    Electronic Resource
    Electronic Resource
    Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis (1996)
    Springer
    Plant cell reports 15 (1996), S. 869-872 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or PAA. Shoots were induced only when leaf derived callus was subcultured on medium containing BAP (2.0, 5.0 mg/l) in combination with PAA (0.5, 1.0 mg/l). No shoot regeneration was observed when 2,4-D, NAA or BAP were used in the medium either singly or in different combinations. These results demonstrate that PAA in combination with BAP was essential to trigger shoot regeneration from cultured leaf callus of D. chinensis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233159
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  • 3
    Electronic Resource
    Electronic Resource
    High frequency embryoid and plantlet formation from tissue cultures of the Finger millet-Eleusine coracana (L.) Gaertn (1990)
    Springer
    Plant cell reports 9 (1990), S. 93-96 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Compact nodulated embryogenic callus differentiated from cultured seeds of Eleusine coracana (Finger Millet) on Murashige and Skoog (1962) basal medium with 2,4-dichlorophenoxyacetic acid (1.0, 3.0 mg l). This embryogenic callus was maintained on a medium with a lower level of 2,4 — dichlorophenoxyacetic acid. At every subculture the embryogenic callus had some preexisting embryoids in it. With this method of subculture the callus has retained its morphogenic potential for four years. Following transfer to media with different levels of auxins and cytokinins, the callus showed varied patterns of growth and morphogenesis. Embryoids could be germinated in profusion to form plantlets which could be transferred to the field. Shoot buds also differentiated from the whole surface of the embryoid or from the flattened meristemoids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231557
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  • 4
    Electronic Resource
    Electronic Resource
    Embryogenesis in suspension cultures of Datura innoxia Mill. (1993)
    Springer
    Plant cell reports 12 (1993), S. 581-584 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in culture. Embryos differentiated and matured in the liquid medium itself as also evidenced by histological observation. Embryos germinated to form plantlets on semisolid MS medium without growth substances. The regenerated plants had haploid number of chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233065
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  • 5
    Electronic Resource
    Electronic Resource
    Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. ch 1039) (1995)
    Springer
    Plant cell reports 14 (1995), S. 245-248 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (Oryza sativa L. cv. CH 1039). Compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. Murashige and Skoog's (MS) medium supplemented with 2.5mg/1 2,4-D was used as callus induction medium. Plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to MS basal medium supplemented with 2.0mg/1 BAP and 0.5mg/1 NAA in combination. Average number of regenerated plants from one coleoptile ranged from9.1 to 14.0.Four day old coleoptiles showed the highest frequency of plant regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233642
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  • 6
    Electronic Resource
    Electronic Resource
    Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. (1999)
    Springer
    Plant cell reports 19 (1999), S. 64-68 
    Details
    ISSN: 1432-203X
    Keywords: Key words Capsicum annuum L. ; Chilli pepper ; Plant regeneration ; Organogenesis ; Phenylacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A highly efficient three-stage protocol for the regeneration of chilli pepper (Capsicum annuum L.) from cotyledon explants was developed. This protocol used PAA in both the shoot-bud induction medium and the medium for elongation of the shoot buds. A superior medium for the induction of buds from the cotyledons was MS medium supplemented with BA (5 or 7 mg/l) + PAA (2 mg/l). Buds were elongated during the second stage on medium containing BA (2 or 5 mg/l) + PAA (2 mg/l). On this medium most of the buds elongated, and their number also increased due to the formation of new buds; bud elongation was achieved in 100% of the cultures provided the buds were induced in the primary stage on a medium supplemented with BA+PAA. The shoots that elongated in the second-stage rooted at 100% frequency on a medium supplemented with NAA (1 mg/l). The complete plantlets with well-developed root and shoot systems were transferred to field conditions where they grew to maturity, flowered and fruited normally. While shoot-bud induction from the cultured cotyledons was also observed on media supplemented with BA (5 or 7 mg/l) alone or in combination with IAA (0.2–2 mg/l), buds induced on these media were often distorted, with most not developing into normal shoots in the second-stage subculturing; a rosette of buds was seen in the second stage subculturing. On the other hand, PAA in combination with BA in the primary induction medium and second-stage medium promoted normal development and the elongation of shoot buds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050711
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  • 7
    Electronic Resource
    Electronic Resource
    Comparison of developmental stages of inflorescence for high frequency plant regeneration in Triticum aestivum L. and T. durum Desf. (1995)
    Springer
    Plant cell reports 15 (1995), S. 227-231 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Whole immature inflorescences at 4 different developmental stages (0.5, 1.0, 1.5, 2.0 cm in size) of different genotypes of Triticum aestivum and T. durum were cultured to see the morphogenetic responses on Murashige and Skoog's (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (2.5 mg/l). Very young inflorescences 0.5 and 1.0cm long formed embryogenic callus from their entire surface while 1.5 and 2.0 cm long inflorescences formed embryogenic callus from the basal spikelets and rachis only. This embryogenic callus was maintained by regular subcultures on MS medium with 2,4-D (2.5 mg/l) for more than a year. Plantlets were regenerated by transferring the embryogenic callus on hormone-free MS medium. Inflorescences (0.5 and 1.0 cm long) responded best in forming callus as well as plantlets at a very high frequency. Variation in response was observed amongst the genotypes but the qualitative response of formation of embryogenic callus and later regeneration of plantlets was observed from all the genotypes. Immature young inflorescence explants could provide a suitable material for particle gun mediated genetic transformation in wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193725
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  • 8
    Electronic Resource
    Electronic Resource
    Selection of Daucus carota somatic hybrids using drug resistance markers and characterization of their mitochondrial genomes (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 494-502 
    Details
    ISSN: 1432-2242
    Keywords: Amino acid analog resistance ; Herbicide resistance ; Glyphosate ; 5-methyltryptophan ; Sodium selenate ; Selenocystine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from different Daucus carota L. cell strains carrying resistance to glyphosate, 5-methyltryptophan, sodium selenate or selenocystine were fused in three combinations using dextran. Clones were selected for both of the resistances carried by the individual parental strains in medium with both inhibitors. No doubly resistant colonies formed from unfused controls or from protoplasts from each individual parental strain alone. Suspension cultures from the selected clones contained predominantly the additive chromosome numbers of the parental strains. Apparently the four resistances used are expressed dominantly in fusion hybrids. Analysis of mitochondrial DNA showed that recombination occurred in one fusion combination since the mitochondrial DNA in the hybrid cells was different from that of either parent as shown by restriction endonuclease fragment analysis. Mitochondrial DNA in the other two somatic hybrid combinations was parental. Thus, a dominant, nuclear resistance marker system has been developed to select efficiently for somatic hybrids in which mitochondrial DNA recombination can be studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289531
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  • 9
    Electronic Resource
    Electronic Resource
    Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. mathania) (1989)
    Springer
    Plant cell, tissue and organ culture 16 (1989), S. 47-55 
    Details
    ISSN: 1573-5044
    Keywords: Capsicum annuum L. ; callus ; differentiation ; plant regeneration ; shoot buds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leaf, stem, hypocotyl, cotyledon, root, shoot tip and embryo explants of Capsicum annuum L. cv. mathania were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) or kinetin (Kin) alone or in combination with 3-indoleacetic acid (IAA), 3-indolebutyric acid (IBA), α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). BAP (5.0 mgl−1) in the medium was found to be the best growth regulator for shoot bud differentiation. Shoot buds cultured on 5.0 mgl−1 BAP increased in number but did not elongate. For obtaining complete plantlets, shoot buds were placed on a medium with IBA or NAA (0.1 mgl−1). Histological evidence revealed direct differentiation of buds from cotyledons. Regenerated plants were normal diploids. Unorganized callus could not be induced to differentiate shoot buds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044071
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