ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

A simple enzyme immunoassay for detecting brucellosis antibodies (1992)

Krambovitis, Elias ; Tselentis, John ; Tsoukatos, Debbie

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 89 (1992), S. 0

add to mindlist on the mindlist

Details

ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract A simple enzyme immunoassay was developed and evaluated for serological diagnosis of brucellosis in 25 patients with various forms of brucellosis and 292 control patients with other conditions and disorders. All brucellosis patients gave a positive test with the initial sample. In 3 acute, febrile brucellosis patients with follow-up sera taken during therapy a sharp drop in specific antibody was noted. There was a less pronounced antibody reduction in 1 chronic and 2 relapse patients and an antibody increase in 1 chronic and 1 relapse case. All control samples gave negative results. In addition, the assay was evaluated as a screening test with 315 sera from ‘healthy’ individuals living in a brucellosis focus and representing 15% of that population. 11.5% (34/293) of subjects with no reported history of brucellosis and 45% (10/22) of cases treated in the past gave a positive test result. The agreement in those samples between the assay and the serum agglutination test was 95.5%.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb04979.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

A tandem repeat of MUC1 core protein induces a weak in vitro immune response in human B cells (1999)

Andersson, Eva ; Henderikx, Paula ; Krambovitis, Elias ; [et al.]

Springer

Cancer immunology immunotherapy 47 (1999), S. 249-256

add to mindlist on the mindlist

Details

ISSN: 1432-0851

Keywords: Key words In vitro immunisation ; MUC1 ; Human ; antibodies ; Phage display ; Carcinoma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract We have recently described an efficient method to study the human humoral immune response in vitro and to generate isotype-switched, antigen-specific human B cells, which has allowed us to produce high-affinity IgG antibodies against different peptides. In an attempt to study the in vitro immune response against self-antigens, such as tumour-associated antigens, this protocol was used to immunise resting human peripheral blood B cells with a peptide epitope from the human-adenocarcinoma-associated antigen, MUC1. After the two-step in vitro immunisation, the secondary immunised cultures were tested for MUC-1-specific antibodies by enzyme-linked immunosorbent assay (ELISA). Phage molecular libraries were subsequently constructed, using the variable parts of Ig genes derived from cells taken from ELISA-positive wells. The libraries were selected on the MUC1 core peptide. Antigen-specific Fab fragments, specific for the self antigen MUC1, were found in the library of secondary immunised IgG+ B cells and these antibodies were evaluated by BIAcore analysis. The specific Fab fragments exhibited an unusually rapid dissociation rate constant and the overall response frequency was lower, as compared to other antibodies generated by this protocol, which might be explained by the repetitive nature of the core peptide used for immunisation.