ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: The present study investigated the effects of flupirtine(Katadolon) on tumor necrosis factor (TNF)-α-mediated cell death andBcl-2 expression in the permanent rat oligodendrocyte cell line OLN-93 (OLNcells). TNF-α (500 U/ml) induced apoptosis of OLN cells, which wasconfirmed by DNA fragmentation using an in situ end-labeling technique andultrastructural analysis. Flupirtine significantly reduced the rate ofspontaneous cell death of OLN cells already at low concentrations;TNF-α-mediated apoptosis was suppressed only with higher concentrationsof flupirtine (100 μM). Expression of Bcl-2 protein and mRNA inOLN cells was detected by immunocytochemistry, western blot, and RT-PCR.Quantitative analysis of western blots revealed an ∼2.5-fold up-regulationof Bcl-2 protein during TNF-α treatment. Furthermore, addition of 10 or100 μM flupirtine before incubation with TNF-α led to anapproximately threefold increase of Bcl-2 expression. Exposure of OLN cells toflupirtine alone moderately augmented the expression of Bcl-2 protein. Ourdata demonstrate that flupirtine up-regulates the expression of Bcl-2 proteinin OLN cells; this Bcl-2 induction is associated with a reduced rate ofTNF-α-induced cell death.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1471-4159.2000.0752270.x
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