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  • 1
    Electronic Resource
    Electronic Resource
    in Vitro Effect of Deoxynivalenol on the Differentiation of Human Colonic Cell Lines Caco-2 and t84 (1998)
    Springer
    Mycopathologia 142 (1998), S. 161-167 
    Details
    ISSN: 1573-0832
    Keywords: Caco-2 cells ; deoxynivalenol ; differentiation ; enterocytes ; T84 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of low concentrations of deoxynivalenol (DON) on structural and functional characteristics of human colonic adenocarcinoma cell lines Caco-2 and T84 were examined. Scanning electron microscopic (SEM) analysis of the apical surfaces of Caco-2 cells revealed reduction or abnormal formation of brush borders in the presence of 50, 100 and 200 ng/ml of DON. Monolayer integrity of Caco-2 and T84 cells was studied using cells which were cultured on permeable membranes. The transepithelial electrical resistance (TEER) of Caco-2 cells was significantly reduced at 50, 100 and 200 ng/ml of DON, significant increase in lucifer yellow (LY) permeability was also observed in these cells at 100 ng/ml of DON. The TEER of T84 cells was significantly reduced at 100 and 200 ng/ml of DON. LY permeability significantly increased at 200 ng/ml of DON in T84 cells. Enzyme activities in Caco-2 cells were also examined. Alkaline phosphatase activity was reduced from the 6th to 15th day of culture in the presense of 100 or 200 ng/ml of DON, whereas sucrase- isomaltase activity was significantly decreased by adding 50 or 100 ng/ml of DON for 15 or 20 days. Protein content was attenuated only by treatment with 200 ng/ml of DON thoughout the experimental period. The results indicate that DON interferes with structural and functional characteristics of differentiation in enterocytes at low doses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006923808748
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  • 2
    Electronic Resource
    Electronic Resource
    Neonatal exposure to zearalenone causes persistent anovulatory estrus in the rat (1982)
    Springer
    Archives of toxicology 50 (1982), S. 279-286 
    Details
    ISSN: 1432-0738
    Keywords: Zearalenone ; Rat ; Neonatal administration ; Persistent estrus ; Anovulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of neonatal administration of zearalenone on the female reproductive system was studied in the rat. A single subcutaneous injection of 1.0 mg zearalenone to 3- or 5-day-old rats caused persistent vaginal estrus in adulthood. Ovaries in these animals contained many large follicles but no newly formed corpora lutea. The same effects were observed in rats which had received 100 μg estradiol-17β in the neonatal period. Most rats which had received 100 μg zearalenone or 10 μg estradiol-17β showed regular 4-day estrous cycles and had newly formed corpora lutea in their ovaries. These results demonstrate that neonatal exposure to zearalenone produces persistent anovulatory estrus in the rat, the potency being about one tenth that of estradiol-17β.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310860
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of Fusarenon-X and T-2 toxin on intestinal absorption of monosaccharide in rats (1988)
    Springer
    Archives of toxicology 61 (1988), S. 489-495 
    Details
    ISSN: 1432-0738
    Keywords: Fusarenon-X ; T-2 Toxin ; Jejunal absorption ; Monosaccharide ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to delineate the effects of Fusarenon-X (FX) and T-2 toxin (T-2) on intestinal absorption of monosaccharide, jejunal absorption in vivo and uptake in vitro of 3-0-methyl glucose (m-glucose) and l-glucose were studied in rats. Jejunal absorption in vivo was assessed by determining the rate of appearance of the sugars in plasma of the mesenteric vein draining the jejunal segment, which was perfused with the medium containing radiolabelled m-glucose or l-glucose. Jejunal uptake in vitro was assessed by determining the m-glucose or l-glucose uptake by the everted jejunum taken from toxin-treated rats, m-Glucose absorption was reduced 1 or 3 h after either toxin was injected into the jejunal lumen. A reduction of m-glucose absorption was also noted after intravenous injection of the toxins, although the timing and magnitude of the reduction were slightly different from those seen after the luminal injection. These results suggest that both toxins impair the jejunal function relating to monosaccharide absorption in the early stages of intoxication. The reduction in m-glucose absorption was associated with a reduction in l-glucose absorption and unchanged or increased uptake of l-glucose. The active transport component, which was indicated by the difference between absorption or uptake of the two sugars, was also reduced in association with the reduction of m-glucose absorption. These results suggest that the toxins cause specific damage in the active transport system for monosaccharides on the one hand, and impairment of their diffusional movement from the epithelial layer to the mesenteric vein on the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293696
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro microsome-mediated aflatoxin B1-DNA binding and its inhibition by cytosol of various organs of the hamster and quail (1995)
    Springer
    Mycopathologia 132 (1995), S. 117-119 
    Details
    ISSN: 1573-0832
    Keywords: Activation ; Aflatoxin B1 ; Cytosol ; Hamster ; Microsome ; Quail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We studied thein vitro activation of aflatoxin B1 (B1) by microsomes and its inactivation by the cytosol of various quail and hamster organs, using B1-DNA binding as an index. The microsomal activity of the liver to bind B1 to DNA was not largely different between the two species and was higher than that of the other organs examined in either species. The microsomal activity of the kidney and lung was very low in the quail compared with the hamster, indicating the very small contribution of the lung and kidney microsomes to the activation of B1 in birds. Only the hamster liver cytosol showed strong inhibition of microsome-mediated B1-DNA binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103784
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