ISSN:
1573-6784
Keywords:
apoptosis
;
cell-free extract
;
proliferation inhibition
;
protease B
;
skimmed milk
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Apoptosis-inducing materials were produced by digesting bovine skimmed milk with cell-free extract of Saccharomyces cereviiae at pH 4.8. An enzyme involved in production of the materials was purified from the cell-free extract by successive column chromatography. The purified enzyme was homogeneous and identified as protease B by analyzing N-terminal amino acid sequence. Characteristics features of apoptosis were observed within 5 h of digested skimmed milk treatment as documented by DNA fragmentation, expression of phosphatidylserine. The inducing factors were recovered in the soluble fraction of 92% ethanol, suggesting that the factors were hydrophilic low molecular weight substances.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008946616614
Permalink
