Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Über den Einfluß der Gelinkubation mit Polyvinylalkohol auf die Löslichkeit der Fructose-1,6-diphosphat-Aldolase (1968)
    Springer
    Histochemistry and cell biology 13 (1968), S. 196-202 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Verminderung der Löslichkeit der Fructose-1,6-diphosphat-Aldolase (Fructose-1,6-diphosphat-D-Glycerinaldehyd-3-phosphat-Lyase, E.C. 4.1.2.13) durch Zusatz von Polyvinylalkohol zum Inkubationsmedium wurde unter Einhaltung histochemischer Bedingungen überprüft. Bei der Inkubation in wäßrigem Medium sind nach 100 min in einem Schnitt des Herzmuskels und der Niere der Ratte weniger als 10% der ursprünglichen Enzymaktivität nachweisbar. Nach Zusatz von Polyvinylalkohol zum Inkubationsmedium beträgt die im Schnitt verbleibende Enzymaktivität nach gleicher Inkubationsdauer für den Rattenherzmuskel etwa 80%, für die Niere 30–40%. Die Bedeutung dieser Befunde für den histochemischen Nachweis der Aldolase wird am Beispiel des Rattenherzmuskels gezeigt. Als Ursache der organbedingten Unterschiede im Löslichkeitsverhalten wird die Anwesenheit verschiedener Isoenzyme in beiden Organen in Betracht gezogen.
    Notes: Summary The decrease in the solubility of fructose- 1,6-diphosphate-aldolase (fructose-1, 6-diphosphate-D-glyceraldehyde-3-phosphate lyase, E.C.4.1.2.13) brought about by the addition of polyvinyl alcohol to the incubation medium was tested observing histochemical conditions. After 100 minutes of incubation in an aqueous medium less than 10% of the original enzymic activity was demostrated in sections of rat cardiac muscle and kidney. After polyvinyl alcohol was added to the incubation medium the enzymic activity in the sections (measured over the same period) was 80% in rat cardiac muscle and 30–40% in kidney sections. The importance of these findings for the histochemical demonstration of aldolase is shown in the rat cardiac muscle. The organ dependend differences in the solubility may find an explanation in the presence of various iso-enzymes in the two organs under investigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266581
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Zur problematik des histotopochemischen nachweises der fructose-1,6-diphosphat-aldolase am beispiel der rattenniere (1967)
    Springer
    Histochemistry and cell biology 9 (1967), S. 84-92 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die bisher bestehenden histochemischen Nachweismethoden für die Fructose-1,6-diphosphat-Aldolase weisen bezüglich einer genauen Enzymlokalisation mehrere Fehlermöglichkeiten auf, die im einzelnen aufgezeigt werden. Ein von Simon u. Mitarb. angegebenes indirektes Nachweisverfahren ist bei Einhaltung folgender Bedingungen zur ortsrichtigen Wiedergabe der Enzymaktivität geeignet: 1. Inkubation in einem Polyvinylalkohol-Gel zur Verminderung der Diffusion des Fermentes ins Inkubationsmedium; 2. Substitution der Tetrazoliumsalzreduktase durch Phenazinmetosulf at; 3. Hemmung der Cytochromoxydase durch Kaliumcyanid. Man erhält auf diese Weise eine gleichmäßige starke Enzymaktivität in allen Tubulusabschnitten, eine kräftige Darstellung der Arterienwände sowie eine schwache Aktivität der Glomerula und des kapillarführenden Interstitiums.
    Notes: Summary All methods employed in the histochemical determination of fructose-1.6-diphosphate-aldolase show certain deficiencies which make a correct localization of the enzyme rather difficult. However, a correct topographical localization of the enzymatic activity is possible by employing the indirect method devised by Simon et al., provided the following facts are observed: 1. incubation in polyvinyl-alcohol-gel to reduce the diffusion of the enzyme into the incubation medium. 2. substitution of tetrazoliumsalt-reductase by phenazine-methosulphate. 3. inhibition of cytochrome oxidase by potassium cyanide. Thus an equally strong enzyme activity is demonstrable in all parts of the tubuli; arterial walls show a strong, glomeruli and capillarised interstitium a weak reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281810
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Nuclear image analysis of immunohistochemically stained cells in breast carcinomas (1996)
    Springer
    Histochemistry and cell biology 105 (1996), S. 479-485 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hitherto, the relationship between malignancy-associated morphological features in single tumour cells and the expression of markers indicating functional properties of these cells remained widely unknown. This study was aimed at describing differences in the size, shape and chromatin structure between tumour cells with different marker expression for progesterone receptors (PgR) and p53. Two series of breast cancers, consisting of 50 PgR-positive, and 39 p53-negative and 49 p53-positive mammary carcinomas, were investigated. The immunohistochemical staining was performed on paraffin sections using 3-amino-9-ethylcarbazole as the chromogenic substrate. By means of a cytometry workstation equipped with a computer-controlled motorised scanning stage, about 500 positive and negative tumour cells in each case were localised in the microscope and categorised by a scoring system for their staining intensity. After destaining, the tissue sections were Feulgen-stained. Then, all the tumour cells were relocated automatically and analysed by high resolution image cytometry. Among the numerous size, shape, and texture features used in the system, several variables of the nuclear contour and chromatin structure were found to be significantly different between the positive and negative tumour cell populations. Nuclei without PgR had more malignancy-associated morphological features than PgR-positive cells. Whereas p53-negative nuclei had a higher degree of regularity, their positive counterparts exhibited higher DNA ploidy values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01457662
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Beitrag zur Histochemie der Laktatdehydrogenase (1967)
    Springer
    Histochemistry and cell biology 11 (1967), S. 350-359 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Nach Verminderung der Diffusion des Enzyms in das Inkubations-medium durch Inkubation in einem Polyvinylalkohol-Gel und nach Substitution der NADH2: Tetrazoliumsalzoxidoreduktase durch PMS erhält man mit der konventionellen Methode zum Nachweis der LDH an der Rattenniere ein neuartiges Verteilungsmuster der Gesamt-aktivität, das im Gegensatz zu dem bekannten histochemischen Bild eine gute Übereinstimmung mit quantitativen biochemischen Befunden nach Mikrodissektion von Nierenschnitten zeigt. Unter Einhaltung der obengenannten Bedingungen gelingt an der Rattenniere ebenso wie an der Skeletmuskulatur eine selektive Darstellung des H-Typs der LDH-Isoenzyme, wenn dem Inkubationsmedium Harnstoff zugesetzt wird.
    Notes: Summary A completely novel distribution pattern of the total activity of LDH in the rat kidney was obtained by using polyvinyl alcohol gels as incubation medium (thus preventing the enzyme from diffusing into the incubation medium), substituting NADH2: tetrazolium-oxidoreductase by PMS and using conventional methods for the determination of LDH. In contrast to the usual histochemical findings this method corresponds very well to quantitative biochemical findings after micro-dissection of the renal sections. If the above mentioned conditions are oberserved and urea is added to the incubation medium a selective demonstration of the H-type of LDH-isoenzymes is possible in the rat kidney and skeletal musculature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305811
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...