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  • 1
    Electronic Resource
    Electronic Resource
    Relationship of the pelargonium flower break carmovirus (PFBV) coat protein gene with that of other carmoviruses (1998)
    Springer
    Archives of virology 143 (1998), S. 1823-1829 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The 3′-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation. The sequence also included the putative promoter of the coat protein gene subgenomic RNA, as well as its 5′ and 3′ untranslated regions. The PFBV coat protein was more similar to that of saguaro cactus virus and carnation mottle virus than to that of other carmoviruses. Despite the lower level of similarity of CP gene sequences compared to the RNA dependent RNA polymerase (RdRp) gene sequences of small icosahedral viruses used in taxonomic studies, PFBV CP sequence comparisons and alignments confirmed that PFBV is related to carmoviruses, tombusviruses and a dianthovirus, as previously concluded from the analysis of a PFBV RdRp gene fragment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050420
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Field resistance against potato virus Y infection using natural and genetically engineered resistance genes (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 377-382 
    Details
    ISSN: 1573-8469
    Keywords: Nicotiana tabacum ; coat protein mediated protection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Commercial tobacco cultivars BB16 (burley) and PBD6 (dark air cured) were transformed with the coat protein gene of lettuce mosaic potyvirus (LMV). Transgenic BB16 plants showed resistance to potato virus Y (PVY) infection, against the necrotic strain PVY-N Versailles, as well as the resistance breaking necrotic strain PVY-N 107. Transgenic PBD6, which carries the recessive ‘va’ gene conferring resistance to PVY, was also protected against PVY-N 107. In the progenies of most BB16 and PBD6 transformants, 45% to 100% of the inoculated plants were immune to PVY infection. The rest of the plants were tolerant, with atypical attenuated non necrotic symptoms and reduced virus accumulation. No recovery was observed in the tolerant plants, which stably expressed detectable level of LMV coat protein. This suggested a protein-mediated mechanism of heterologous protection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008079003061
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    Paper (German National Licenses)
    Fulltext
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