ISSN:
1573-7373
Keywords:
brain tumor
;
(14C-methyl)-L-methionine
;
cycloheximide
;
protein synthesis
;
autoradiography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Autoradiographic study of (14C-methyl)-L-methionine with brain tumor bearing rats aimed at an elucidation of the mechanism of tracer accumulation in the protein synthesis of tumor. Twice as much tracer accumulated in the tumors compared as in the contralateral gray matter (nontumor region) at 90 min post intravenous injection. The protein-bound fraction of the tumors, expressed as acid-insoluble fraction (AIF), was 1.7 ± 0.6 (mean ± standard deviation, n=6), significantly higher than that (0.8 ± 0.2) of the nontumor region (p〈0.05 by the Mann-Whitney test). The tumor AIF comprised 82.3 ± 9.2% of the total amount of the tracers accumulated in the tumors. The protein synthesis inhibitor cycloheximide reduced the tracer uptake and the AIF of the tumors to an almost same level as the nontumor region. These findings indicate that metabolic acceleration of protein synthesis may be a main reason for the high accumulation of (14C-methyl)-L-methionine in tumor.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01050068
Permalink
