ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
A murine monoclonal antibody (MoAb) F101 01 reacting with the T cell receptor (TCR)-T3 complex is presented. Immunohistological studies showed that FH101.01 specifically stains T-zone lymphocytes in lymph nodes, tonsils, and splenic tissue. Two-colour immunofluorescence and flow cytometry demonstrated co-expression of the antigen defined by F101.01 and the pan-T cell antigens defined by CD2, CD3, CD5 and CD7 antibodies. Cells Stained with CD4 and CDS antibodies were both included in the F101.01-positive population, whereas CD16-positive natural killer cells (NK). B cells (CD19 and CD20), and myeloid cells (CD13 and CD33) were excluded. The target antigen of F101.01 comodulated with the CD3-defined antigen (T3) and the TCR recognized by the MoAb WT-31. CD3 antibody and WT-31 both blocked binding or F101.01. F101.10 precipitated the TCR-T3 complex from lysates of 125-labelled peripheral blood mononuclear cells (PBMC) and HPB-ALL, when the lysate was prepared with a detergent (digitonin) that conserves the TCR-T3 complex. FACS analysis of T cells from a patient with a T cell immunodeficiency demonstrated that δ-TCS-1-CD3-CD4- and δ-TCS-1-CD3+CD8+ cells were brightly F101.01+, whereas a large subpopulation of δ-TCS-1+CD3+CD4− CD8− cells were weakly F101.01+. We conclude that F101.01 recognizes a conformational epitope of theTCR-T3 complex and that it reacts with the αβ TCR-T3 and the γδ TCR-T3 complexes with different intensities.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1988.tb02402.x
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