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  • 1
    Electronic Resource
    Electronic Resource
    Interference by Rheumatoid Factor and Clq in the Detection of IgG Complexes: Studies of Model Systems by ELIS A (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 15 (1982), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Interference by purified IgM rheumaloid factors (RFs) and Clq in the detection of model complexes was studied by enzyme-linked immunosorbent assay (ELISA). Soluble monoclonal and polycloral cryoglobulin IgM RFs und human or porcine Clq inhibited dosedepcndently the binding of human IgG complexes to solid-phase IgM RF, Clq, and bovine conglutinin (Kg). The inhibition patterns of the Kg binding were dependent on the order of confrontation between the reactants. To achieve inhibition, Clq or RF had to he offered lo the complexes before treatment with fresh normal human serum (NHS); complexes pretreated with fresh NHS (alexinated complexes) were resistant to inhibition. Heating at 56 °C or 63 °C, which destroyed the alexinating capacity of NHS, did not affect the Kg binding of alexinated complexes. On the basis of present and previous findings it is concluded that intrinsic Clq and RFs are likely to interfere with detection of circulating immune complexes (CICs) in Clq- or RF-binding assays; they are less likely to interfere with detection of CICs in Kg-binding assays. The problems associated with selective removal of intrinsic Clq and RFs are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1982.tb00655.x
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  • 2
    Electronic Resource
    Electronic Resource
    IgG Autoantibody to Human Serum Albumin Studied by the ELISA-Technique (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 7 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: ELISA was applied for analysis of the HSA-human IgG autoantibody system responsible for the immunoelectrophoretic “Tailing Albumin” (TA) phenomenon induced in most of the TA patients by prolonged nitrofurantoin therapy. Both hyperimmune porcine anti-HSA and autoimmune human anti-HSA antibodies of the IgC class were detectable by ELISA. The presence of autologous or added HSA had some inhibitory effect upon the detectability of the anti-HSA antibodies. Partial elimination of the autologous HSA by sucrose gradient ultracentrifugation or salt precipitation increased or unmasked the anti-HSA activity of some TA sera. The sensitivity of the ELISA as detector of the anti-HSA autoantibodies of whole human sera was roughly equal to that of the immunoelectrophoretic TA phenomenon. The analogy of The anti-HSA autoantibodies and the rheumatoid factors and the theoretical interest of both of them is stressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb00472.x
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  • 3
    Electronic Resource
    Electronic Resource
    Circulating Immune Complexes and Rheumatoid Factors in Patients with Farmer's Lung (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 38 (1983), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Circulating IgG immune complexes (CICs), IgM rheumatoid factors (RFs), complement level (C3 and C4), and IgG antibodies to Aspergillus fumigatus, Aspergillus umbrosus, Thermo-actinomyces vulgaris, and Micropolyspora faeni were analysed in the sera of 14 patients with farmer's lung (FL), 10 in the acute and four in the subacute phase of the disease. Ten spouses of FL patients served as exposed healthy controls. C3 and C4 were measured fluoronephelometrically. C3 levels were above the normal range and C4 levels near the upper limit of the normal range in both patients and controls; no statistically significant difference between patients and controls were observed. CICs were determined by enzyme immunoassays (EIA) of conglutinin-binding (KgB) and Clq-binding (ClqB). CIC levels above the normal range were detected in 12 (KgB-EIA) and nine (ClqB-EIA) of the patients and three (KgB-EIA) and six (ClqB-EIA) of the controls. No statistically significant differences were found in the mean levels between patients and controls. In contrast, RF levels in the acute phase of FL were significantly higher in the patients (P 〈 0.01) than in the controls. CICs correlated positively with most microbial IgG antibodies, but negatively with RFs. RFs also correlated negatively with microbial IgG antibodies, as well as with both C3 and C4. In FL, the increased RF level may, in the absence of increased CIC and decreased complement levels, represent an immune response (IgM anti-IgG autoantibodies) induced by local mechanisms of IgG immune complexes in the lungs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1983.tb04129.x
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  • 4
    Electronic Resource
    Electronic Resource
    Rapid Adhesive Responses of Endothelial Cells and of Neutrophils Induced by Leukotriene B4 are Mediated by Leucocytic Adhesion Protein CD18 (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 31 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Controversy has existed as to the ability of leukotriene B4(LTB4) to enhance adhesive properties of human neutrophils (PMN) and endothelial cells. We found that LTB4 induced a rapid but transient adhesion of PMN to an albumin-coated plastic surface and to cultured human umbilical vein endothelial cells (HUVEC). Although the adhesive response of PMN to the chemotactic peptide N-formyl-methionyl-leucyl-phenylalaninc (fMLP) was longer lasting, peak hyperadherence was of similar magnitude as to LTB4 and was less susceptible to assay conditions. Adherence induced by either LTB4 or fMLP could be abrogated by the monoclonal antibody 60.3, indicating similar dependence on the leucocyte adhesion protein CD18. Lipoxin A did not induce PMN hyperadherenee. Treating HUVEC with LTB4, but not with its omega-oxidized metabolites 20-OH- and 20-COOH-LTB4, lipoxin A, or with IMLP conferred a rapid, dose-related, enhanced adhesion of PMN. This effect was dependent on CD18 and on divalent cations. It disappeared with prolonged exposure to LTB4, required a metabolically active HUVEC, and was not due to passive binding of LTB4 lo HUVEC. Thus, LTB4 induces a transient expression of hyperadhesiveness in HUVEC as well as in neutrophils, and both effects are dependent on expression of CD18.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02825.x
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  • 5
    Electronic Resource
    Electronic Resource
    Opposite effects of 5-hydroxytryptophan and 5-hydroxytryptamine on the function of microdissected ob/ob-mouse pancreatic islets (1983)
    Springer
    Diabetologia 24 (1983), S. 52-57 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; ob/ob mouse ; insulin release ; calcium uptake ; 5-hydroxytryptophan ; 5-hydroxytryptamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of 5-hydroxytryptamine and 5-hydroxytryptophan on insulin release and 45Ca2+ uptake in islets microdissected from ob/ob mice were studied. At a concentration of 4 mmol/l both compounds slightly stimulated insulin release at a low glucose concentration (3 mmol/l). Insulin release induced by 20 mmol/l D-glucose was inhibited by 4 mmol/l 5-hydroxytryptamine but potentiated by 4 mmol/l L-5-hydroxytryptophan. Mannoheptulose (20 mmol/l) blocked the combined effects of 20 mmol/l D-glucose and 4 mmol/l L-5-hydroxytryptophan on insulin release. 45Ca2+ uptake was inhibited by 4 mmol/l 5-hydroxytryptamine and stimulated by 4 mmol/l L-5-hydroxytryptophan. Mannoheptulose (20 mmol/l) did not affect the 45Ca2+ uptake induced by the latter. When 4 mmol/l L-5-hydroxytryptophan was present only during the 30-min preincubation period, 20 mmol/l-glucose-induced insulin release and 45Ca2+ uptake during a subsequent incubation period were inhibited. Externally added 5-hydroxytryptamine (4 mmol/l) did not change the effects of 4 mmol/l L-5-hydroxytryptophan on insulin release and 45Ca2+ uptake. It is concluded that, when added directly into the incubation medium, 5-hydroxytryptophan has effects on insulin release and 45Ca2+ uptake which are opposite to those observed when 5-hydroxytryptamine is added. These effects do not seem to be mediated by 5-hydroxytryptamine formed intracellularly from 5-hydroxytryptophan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275948
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