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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 26 (1972), S. 163-198 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Atlantic salmon, Salmo salar L., brown trout, S. trutta L. and their hybrids are normally identified in the field by empirical differences in maxilla length, thickness of the caudal peduncle, degree of forking of the tail and overall body conformation. This study quantifies these characters and analyses their variations in electrophoretically identified hatchery-reared individuals. Means and variances of the various measures are presented for salmon, trout and hybrids separately. The morphometric characters do not satisfactorily distinguish hybrids from the pure species, and even within samples of the pure species, some individuals will be misclassified as hybrids, or as members of the opposite species, if single characters are used on their own. Hybrids often resemble one or other parent species in one or more characters and are less often intermediate in phenotype. Triploidized hybrids are more like salmon than diploid hybrids are, and triploidized salmon are not different from diploid salmon. The results confirm that frequencies of hybrids of these species cannot be reliably assessed by morphological characters alone, and even for individuals of the pure parental species, independent confirmation of species status is advisable. Early reports of hybrid frequencies in wild stocks should be treated with caution, and apparently higher levels of hybridization in more recent studies compared with older, traditional surveys may simply reflect the greater precision of electrophoretic identification.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of bioenergetics and biomembranes 3 (1972), S. 235-243 
    ISSN: 1573-6881
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Mitochondria isolated from the yeastSaccharomyces cerevisiae were negatively stained with ammonium molybdate. Extensive headpiece-stalk projections were observed lining the disrupted mitochondrial membranes. These observations represent the first clear demonstration of headpiecestalk particles on yeast mitochondrial membranes. Phosphotungstic acid was somewhat less satisfactory than ammonium molybdate in the visualization of the headpiece-stalk particles. Mitochondria isolated from glucose-repressed cells and oligomycin-resistant mutant cells were also examined by negative staining and found to show numerous headpiecestalk elements. Gross differences in the morphology of mitochondria from normal, glucose-repressed and oligomycin-resistant cells, as examined by negative staining, were not apparent in the present studies. The nature and expression of the oligomycin mutation is discussed in terms of possible changes in membrane protein and phospholipid.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of bioenergetics and biomembranes 14 (1982), S. 191-205 
    ISSN: 1573-6881
    Keywords: Plasmalemma redox ; transmembrane dehydrogenase ; proton transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Both respiratory-competent and respiratory-deficient yeast cells reduce external ferricyanide. The reduction is stimulated by ethanol and inhibited by the alcohol dehydrogenase inhibitor, pyrazole. The reduction of ferricyanide is not inhibited by inhibitors of mitochondrial or microsomal ferricyanide reduction. Cells in exponential-phase growth show a much higher rate of ferricyanide reduction. The reduction of ferricyanide is accompanied by increased release of protons by the yeast cells. We propose that the ferricyanide reduction is carried out by a transmembrane NADH dehydrogenase.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Mitochondrial ribosomes have been isolated from five strains of Saccharomyces cerevisiae each carrying a mitochondrial mutation conferring in vivo resistance to spiramycin. 2. The response of poly U directed poly-phenylalanine synthesis to the antibiotics spiramycin, carbomycin and lincomycin was tested using the ribosomes of each mutant. The results indicate that all mutants possess altered ribosomes. Genetic mapping studies suggest that the 21S rRNA may be modified in each mutant. 3. The expression of antibiotic resistance by the mitochondrial ribosomes of some strains is partially or totally dependent upon an association with the mitochondrial membrane. Interpretation of the protein synthesis data is made in terms of the existence of a functional interaction between the ribosomes and the membrane in vivo. 4. Phenotypic interactions have been observed between different ribosomal mutations including ery1, ery2, spi2, spi3 and spi4 and a mutation affecting the mitochondrial membrane, oli. The expression of the ribosomal mutation is often markedly affected by the presence of the oli1 membrane mutation. 5. Membrane effects very similar to those observed following the introduction of the oli1 mutation, such as the unmasking of antibiotic sensitive ribosomal sites, can be obtained by the treatment in vivo of certain antibiotic resistant strains with non-toxic levels of the detergent SDS (〈 0.01%). 6. The SDS effect together with the observation of phenotypic interactions between mutations provide strong supportive evidence of a functional association between a membrane component (in this case of the mtATPase) and a component of the mitochondrial ribosomes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 141 (1975), S. 9-22 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isolation and characterisation of a mutant affecting the assembly of mitochondrial ATPase is reported. The mutation confers resistance to oligomycin and venturicidin and sensitivity of growth on nonfermentable substrates to low temperature (19°). Genetic analysis indicates that the phenotype is due to a single mutation located on the mitochondrial DNA which is probably allelic with the independently isolated oligomycin resistance mutation [oli1-r]. Growth of the mutant at the non-restrictive temperature (28°) yields mitochondria in which the ATPase appears more sensitive to oligomycin than that of the sensitive parental strain. However, when the enzyme is isolated free from the influence of the membrane strong resistance to oligomycin is evident. These data suggest that the component responsible for the oligomycin resistance of the ATPase is part of or subject to interaction with the mitochondrial inner membrane. Measurements of the ATPase content of mitochondria indicate that ATPase production is impaired during growth at 19° C. In addition, studies of the maximum inhibition of mitochondrial ATPase activity by high concentrations of oligomycin suggest a selective lesion in ATPase assembly at low temperature. The nett result is that during growth at 19° only about 10% of the normal level of ATPase is produced of which less than half is membrane integrated and thus capable of oxidative energy production. We propose that the mutation affects a mitochondrially synthesised membrane sector peptide of the ATPase which defines the interaction of F1 ATPase with specific environments on the mitochondrial inner membrane.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 140 (1975), S. 333-337 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mutant has been isolated which carries a nuclear mutation capable of suppressing certain aspects of the phenotype imposed by a specific mitochondrial mutation. The mitochondrial mutation [tso-r] confers cold sensitivity to growth on nonfermentable substrates and resistance to oligomycin. When both the mitochondrial and nuclear mutations are present in the same cell the cell is phenotypically cold resistant but retains a high level of oligomycin resistance. The extent of cold sensitivity suppression is dependent upon other unspecified nuclear genes. The molecular basis for the suppression may involve interactions between cytoplasmic and mitochondrial products necessary for the assembly of mitochondrial ATPase.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 145 (1976), S. 43-52 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative study of eight independently isolated mitochondrial oligomycin resistant mutants obtained from three laboratories show a variety of phenotypes based on cross resistance to venturicidin and sensitivity to low temperature. Analysis of recombination between pairs of markers indicate the existence of at least three genetic classes; class A, cross resistant to venturicidin and including the mutations O III, [oli1-r], [OLG1-R], [tso-r]; class B, mutations O I, [oli17-r], [OLG2-R]; and class C, the mutation O II. The recombination data is consistent with mutations of each class residing in three separate genes, although mutations of class A and B show very close linkage. Recombination in non-polar crosses has demonstrated that markers of all three classes are linked to the mik1 locus in the configuration (AB)-mik1-C. The mapping of this segment with respect to other markers of the mitochondrial genome and the order of classes A and B was established by analyses of co-retention frequencies of markers in primary petite isolates as well as by analysis of marker overlap of genetically and physically defined petite genomes. The unambiguous order ery1-A-B-mik1-C-par was obtained. DNA-DNA hybridization studies using mtDNA isolated from selected petites confirms this map and estimates the physical separation of markers. A reasonable correlation exists in this region of the genome between distances estimated physically by hybridization and genetically by frequency of recombination in non-polar crosses. It is postulated that the oligomycin-mikamycin linkage group represents a cluster of genes involved in determining a number of mitochondrial membrane proteins associated with the mitochondrial ATPase and respiratory complex III.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1615-6102
    Keywords: Plasma membrane ; NADH oxidoreductase ; Superoxide radicals ; Rho 0(ϱ0) cells ; Quinones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have considered the regulatory interrelationship of the plasma membrane oxidoreductase (PMOR) system and the mitochondrial respiratory capacity of human Namalwa (lymphoblastoid) cells. To this end, we made use of mitchondrially respiratory competent (ϱ+) cells and ϱ0 cells, which lack mitochondrial DNA (mtDNA) and consequently mitochondrial respiratory activity. NADH-fer-ricyanide reductase activity of the PMOR system is increased 3-fold in ϱ0 Namalwa cells compared to ϱ+ cells. It is also shown for the first time that addition of coenzyme Q10 and coenzyme Q10-ana-logues, which can rescue ϱ0 Namalwa cells in the absence of pyravate, gives rise to a further 2–3-fold increase in plasma membrane NADH-ferricyanide reductase activity. These systems were examined to determine if there exists a correlation between the regulation of the PMOR system and extracellular Superoxide radical formation as measured with the fluorescence probe L-012. No correlation was found between NADH-ferricyanide reductase activity and extracellular Superoxide radical production. PMOR function in cellular proliferation appears therefore not to involve extracellular Superoxide radical production.
    Type of Medium: Electronic Resource
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