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  • 1
    ISSN: 0168-9452
    Keywords: Brassica ; cell cycle asynchrony ; differentiation ; mesophyll and hypocotyl protoplasts ; proplastids and chloroplasts ; somatic hybridization
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two Palaemon prawn species, P. adspersus Rathke and P. squilla (L.), occur along the European Atlantic coast. In areas heavily affected by tides (Netherlands and France), one species, P. squilla, inhabits the intertidal zone, and P. adspersus is found subtidally in the Zostera marina meadows. In areas with small tidal ranges (Sweden) both species occur in the Z. marina zone, and here the body size of P. squilla is reduced, something that in turn reduces reproductive output in this species. Is adaptive improvement in non-tidal P. squilla populations prevented by too extensive gene flow from tidal areas? To wanswer this, genetic differentiation was studied by horizontal starch gel electrophoresis. Each of the two species was sampled at five sites along the European Atlantic coast. Twenty-two enzymes representing 25 loci were scored. Significant genetic heterogeneity between sites was found in both species. Thus the extensive gene flow hypothesis was not supported by the results, and non-tidal populations ought to have the possibility to adapt to local conditions.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Key words Asymmetric somatic hybridisation ; Mapped RFLP markers ; Restriction endonuclease ; Ultraviolet irradiation ; X-irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Asymmetric somatic hybrids between Brassica napus (receptor) and Arabidopsis thaliana (donor) have been produced by three different methods supposed to induce asymmetry. The donor protoplasts were either UV- or X-irradiated, or the mixture of protoplasts was treated with the restriction enzyme PvuII immediately before fusion. The genome composition of the hybrids was analysed with Southern blot hybridisations using 15 different mapped A. thaliana RFLP markers as probes. Both UV- and X-irradiation were found to be efficient treatments for induction of asymmetry in somatic hybrids in a dose-dependent manner. The addition of a restriction enzyme to the protoplast mixture did not have any effect on the frequency of asymmetric hybrids or on the degree of asymmetry in the hybrids produced. UV- and X-irradiation resulted in higher fertility in the hybrids, while PvuII treatment did not have any effect on seed set. A significant positive correlation between degree of asymmetry in different plants and seed set after selfing was detected.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic cultures were initiated from mature zygotic embryos of Picea abies. The somatic embryos in the embryogenic cultures were first stimulated to mature and then either to develop further into plantlets or to differentiate new embryogenic cultures. The procedure was repeated three times during two years. The ability to give rise to new embryogenic cultures or to develop into plantlets was similar for all somatic embryos irrespective of how long they had been cultured in vitro. The nuclear DNA content, measured in a flow cytometer, was estimated at 32 pg/G1 nuclei in seedings developed from zygotic embryos. Nuclei isolated from embryogenic cultures and from plantlets regenerated from somatic embryos had the same DNA content as those isolated from seedlings.
    Type of Medium: Electronic Resource
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