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FGF-2 blocks TGF-β1-mediated suppression of Bcl-2 in normal melanocytes (2005)

Von Willebrand, Maria ; Köhler, Klaus ; Alanko, Tuomo ; [et al.]

Oxford, UK; Malden, USA : Munksgaard International Publishers

Experimental dermatology 14 (2005), S. 0

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ISSN: 1600-0625

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: Normal melanocytes require growth support provided by the adjacent basement membrane. In contrast, nevus cells and melanoma cells survive in the dermis, and in vitro on a soft collagen gel. Transforming growth factor-β1 (TGF-β1) produced by melanocytes themselves induces apoptosis in normal melanocytes cultured on collagen gel, an effect that can be counteracted by fibroblast growth factor-2 (FGF-2). The purpose of this study was to investigate the mechanisms by which FGF-2 counteracts the apoptotic signals from TGF-β1 in melanocytes cultured on collagen gel. We report that FGF-2 did not interfere with the signal transduction from the TGF-β1 receptors to SMAD2/3 proteins. Instead, TGF-β1 decreased the level of Bcl-2 in normal melanocytes cultured on collagen gel, and FGF-2 reversed the TGF-β1-mediated reduction in the level of Bcl-2. In nevus and melanoma cells, TGF-β1 was unable to induce a decrease in the level of Bcl-2, and treatment with FGF-2 did not cause an increase in the level of Bcl-2 in nevus or melanoma cells. In conclusion, our results suggest that a reduction in the level of the anti-apoptotic Bcl-2 is involved in the execution of apoptosis induced by TGF-β1 in normal melanocytes cultured on collagen gel and that FGF-2 can prevent TGF-β1 from causing this reduction.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.0906-6705.2005.00277.x

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Transforming growth factor-ßs as modulators of pericellular proteolytic events (1989)

Keski-Oja, Jorma ; Lohi, Jouko ; Laiho, Marikki

Springer

Cytotechnology 2 (1989), S. 317-332

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ISSN: 1573-0778

Keywords: transforming growth factor-ß (TGFß) ; TGFα, proteolysis ; growth factor ; growth inhibitor ; invasion ; metastasis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract Since the discovery of transforming growth factor-ß:s an increasing number of different biological effects have been attributed to this group of proteins. Analysis of the cellular responses to TGFß stimulation at the molecular level has indicated that TGFß acts as an activator of transcription of several genes. This may in part explain the plethora of various functions that have been ascribed to TGFß. In addition to the TGFß family of polypeptides there is an increasing number of related factors, whose major roles appear to be involved in developmental processes. A distinct feature of TGFß is its ability to regulate pericellular proteolysis of cultured cells. As yet this property has not been associated with other members of this group of polypeptides. Depending on the target cell type TGFß may either increase or decrease pericellular proteolytic activity. Proteolytic activation of latent TGFß and its possible inhibition by TGFß-induced protease inhibitors could be a physiological feed-back mechanism in the control of proteolytic activity in the vicinity of cells.