Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    A direct selection procedure for isolating yeast mutants with an impaired segregation of artificial minichromosomes (1989)
    Springer
    Current genetics 15 (1989), S. 17-25 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Minichromosomes ; Impaired segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nondisjunction of artificial yeast minichromosomes (2:0 segregation events) during mitosis is accompanied by the appearance of cells containing more than one copy of the mini-chromosome. A mathematical simulation of this process has demonstrated that under certain conditions, a nondisjunction of the minichromosomes may result in their accumulation in a considerable portion of the cell population. An increase in the copy number of artificial minichromosomes as a result of impaired segregation has been used to develop a new experimental procedure for directly selecting yeast mutants showing an impaired segregation of artificial minichromosomes during mitosis. Four new genes, AMC1, AMC2, AMC3, and AMC4, which control the segregation of artificial minichromosomes in mitosis, have been identified (AMC-3 and AMC4 are mapped to chromosome IV and VII, respectively). Mutations in the genes AMC1–AMC4 also affect the mitotic transmission of natural chromosomes. We suggest that the genes AMC1, AMC2, AMC3, and AMC4 control the segregation of natural chromosomes in yeast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445747
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids (1985)
    Springer
    Current genetics 10 (1985), S. 15-20 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Yeast transformation ; Centromere-containing plasmids ; Mitotic stability of minichromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mutant with unstable maintenance of hybrid plasmids containing either one of the centromeric loci CEN3, CEN6, CEN11 and arsl or the replicator of the 2 μ plasmid has been obtained. The frequency of loss of hybrid plasmids in the mutant was up to 3 · 10−1 per one generation versus 10−2 in the original strain. The unstable maintenance of minichromosomes in the mutant is controlled by a recessive nuclear gene, named SMC for stability of minichromosomes. Loss of some minichromosomes is connected with impairment of their segregation in cell division. In diploids homozygous for smc mitotic chromosomal segregation is not affected but sporulation is impaired. The question of adequacy of usage of minichromosomes for selection of mutants with impaired function of centromeric loci is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418488
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The stability of chromosomes in yeast (1987)
    Springer
    Current genetics 11 (1987), S. 435-443 
    Details
    ISSN: 1432-0983
    Keywords: charomyces cerevisiae ; Yeast transformation ; Centromere-containing plasmids ; Mitotic stability of chromosomes and minichromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary CL mutants with high instability of chromosome III were UV-induced in haploid strain disomic for chromosome III. The obtained CL mutants can be divided into two groups: (1) CL2, CL3, CL7, CL11–L13 with elevated level of spontaneous inter- and intragenic recombination and (2) CL4, CL8 in which instability of chromosome III is not accompanied by elevation of mitotic recombination frequency. CL4 and CL8 mutants also show unstable maintenance of artificial minichromosomes with different chromosomal replicators and centromeric loci. The instability of chromosome III and minichromosomes in CL4 and CL8 is determined by two nonallelic genes designated ch14 and ch18. The role of ch14 and ch18 genes in mitotic chromosome transmission is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384604
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Rapid genetic test for discrimination between haploid and polyploid transformants in Saccharomyces (1984)
    Springer
    Current genetics 8 (1984), S. 341-344 
    Details
    ISSN: 1432-0983
    Keywords: Spheroplast and lithium chloride transformation ; Mitotic homozygotization ; Polyploid transformants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spheroplast fusion induced by polyethylene glycol often occurs during transformation of yeast spheroplasts. It leads to the appearance of polyploid transformants. We have developed a method of discriminating between haploid and polyploid transformants, based on qualitative comparison of the frequency of mitotic homozygotization for a recessive marker, lys2, in diploid and polyploid hybrids of transformants crossed with a tester haploid bearing lys2. We have used this method to compare the frequency of appearance of polyploids during transformations of spheroplasts and of intact cells using lithium chloride in the strain DC5. Polyploids constitute from 15% to 74% of transformants after transformation of spheroplasts. In lithium chloride transformation polyploids arose in only one of four experiments and constituted 9% of transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419822
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Determinant for multiple drug resistance possessing features of a mitochondrial episome in saccharomyces cerevisiae (1977)
    Springer
    Molecular and cellular biochemistry 14 (1977), S. 19-24 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary A mutation for multiple resistance to tetracycline, cycloheximide and oligomycin appears to be followed by reconstruction of the mitochondrial genome resulting in the formation of independent nucleotide sequences that determine different resistant phenotypes. Heterozygotes for the cross resistance factor lack locus T responsible for relation tetracycline which comes from theα-parent. The nuclear recessive gene-suppressori induces deletion of the whole determinant for multiple resistance. The loss of mt-DNA on ethidium bromide treatment does not lead to the loss of this determinant which remains in the cells either in an active or in a passive state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01734160
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Genetic control of chromosone stability in the yeast Saccharomyces cerevisiae (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 4 (1988), S. 257-269 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; yeast ; chromosomes ; cell division ; mitosis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have identified four new genetic loci: CHL2 (on chromosome XII) CHL3 (on chromosomes XII); CHL4 (on chrosomes IV), and CHL5 (on chromosomes IX), controlling mitotic transmission of yeast chromosomes. The frequency of loss of chromosomes is 10-100-fold in chl5, chl2, chl3 and chl4 mutants than observed in wild-type strains. The mutants also unstable maintenance of artifcial circular minichromosomes with various chromosomal replicators (ARS) and one of the concentrations loci (CEN3, CEN4, CEN5, or CEN6). The instability of minichrosomes in the chl5, chl2, and chl4 mutants id due to the loss of minichromosomes in mitosis (1 : 0 segregation). In the chl3 mutant the instability of artificial minichromosomes is due to nondisjunction (2 : 0 segregation). The CHL3 gene therfre appears to affect the segregation of chromosomes during cell division.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320040404
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...