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  • 1
    Electronic Resource
    Electronic Resource
    Ring-opening polymerization of norbornene substituted with amine and ammonium groups (1984)
    s.l. : American Chemical Society
    The @journal of organic chemistry 49 (1984), S. 1886-1890 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00185a008
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  • 2
    Electronic Resource
    Electronic Resource
    Stereoselectivity in norbornene metathesis with Group VI metal-based catalysts. Comparative behavior of cyclic vs. acyclic olefins (1982)
    s.l. : American Chemical Society
    The @journal of organic chemistry 47 (1982), S. 2019-2026 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00132a009
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the behavior of penicillium roquefortii in solid state cultivation on support by material balances
    Amsterdam : Elsevier
    Journal of Fermentation and Bioengineering 74 (1992), S. 305-311 
    Details
    ISSN: 0922-338X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0922-338X(92)90065-3
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  • 4
    Electronic Resource
    Electronic Resource
    Growth and sporulation behaviour of Penicillium roquefortii in solid substrate fermentation: effect of the hydric parameters of the medium (1992)
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 183-187 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Both growth and sporulation increase linearly according to the initial water content of the solid substrate when Penicillium roquefortii is cultivated on buckwheat seeds. This indicates that water is the limiting factor for fungal development since neither carbon nor nitrogen sources were exhausted during these experiments. This feature validates the concept of available water for fungal growth, defined as initial water content of substrate minus its residual water content when vegetative growth stops. An efficient methodology, based on drawing a regression line of mycelium dry weight production as a function of the initial water content of substrate is presented; it allows estimation of both available water and water content of mycelium. Results show that growth stops when the residual water in the substrate is close to 0.52 g H2O/g initial dry matter, corresponding to a water activity (aw) close to 0.96, and that the initial water content in mycelium is near 76%. Thus, both aw and water content of the substrate have to be taken into account during the course of solid-state cultivations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174465
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  • 5
    Electronic Resource
    Electronic Resource
    Fed-batch biotransformation of β-ionone by Aspergillus niger (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 222-227 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Aspergillus niger IFO 8541 was found to be an efficient biocatalyst for the biotransformation of β-ionone into hydroxy and oxo derivatives. The reaction had to be carried out with an inoculum made of about 4×107 fresh spores/l and with a preliminary growth period giving at least 3 g/l biomass. The fungus developed in the form of pellets when cultivated as free mycelium; entrapment of the microorganism in calcium alginate beads was an efficient way to mimic this feature in an aerated, stirred bioreactor. The biotransformation was carried out using a fed-batch mode of operation involving sequential precursor addition. β-Ionone stopped the fungal growth and was converted into metabolites only when the carbon source remained present in the medium; it was fully oxidized after sucrose exhaustion. These conditions allowed recovery of about 2.5 g/l aroma compounds after 230 h cultivation with a molar yield close to 100%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050394
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  • 6
    Electronic Resource
    Electronic Resource
    Pyrazine production by Bacillus subtilis in solid-state fermentation on soybeans (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 489-495 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract 2,5-Dimethylpyrazine (2,5-DMP) and tetramethylpyrazine (TTMP) were produced using Bacillus subtilis IFO 3013 grown on soybeans. Solid-state cultivations were carried out either in 100-ml bottles or in a fixed-bed column reactor, both systems being at 27 °C. Optimization studies showed that the best way to produce the two above aroma compounds involved two separate processes. 2,5-DMP was obtained using soybeans enriched with 75 g threonine/kg initial dry weight (i.d.w.), giving 0.85 g metabolite/kg i.d.w. after 6 days. TTMP production involved addition of 90 g/kg i.d.w. acetoin to soybeans, and 2.5 g/kg i.d.w. was recovered after 14 days. These results demonstrated the suitability of solid-state cultivation for production of high-added-value compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050961
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  • 7
    Electronic Resource
    Electronic Resource
    Fed-batch biotransformation of β-ionone by Aspergillus niger (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 222-227 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus niger IFO 8541 was found to be an efficient biocatalyst for the biotransformation of β-ionone into hydroxy and oxo derivatives. The reaction had to be carried out with an inoculum made of about 4 × 107 fresh spores/l and with a preliminary growth period giving at least 3 g/l biomass. The fungus developed in the form of pellets when cultivated as free mycelium; entrapment of the microorganism in calcium alginate beads was an efficient way to mimic this feature in an aerated, stirred bioreactor. The biotransformation was carried out using a fed-batch mode of operation involving sequential precursor addition. β-Ionone stopped the fungal growth and was converted into metabolites only when the carbon source remained present in the medium; it was fully oxidized after sucrose exhaustion. These conditions allowed recovery of about 2.5 g/l aroma compounds after 230 h cultivation with a molar yield close to 100%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172816
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  • 8
    Electronic Resource
    Electronic Resource
    Spore production ofPenicillium roqueforti by simulated solid state fermentation (1986)
    Springer
    Biotechnology letters 8 (1986), S. 453-456 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A culture technique, based on the growth of a microorganism on inert porous particles (e. g. pozzolano) impregnated and continuously fed with substrate is applied to the growth and spore production ofPenicillium roqueforti. The composition and the feed rate of the medium can be controlled, and the biomass is directly estimated.P. roqueforti exhibits a diauxic growth on the medium containing sucrose and malt extract used, and 1.5 109 spores/g pozzolano may be obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01026754
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  • 9
    Electronic Resource
    Electronic Resource
    Internal substrate concentrations during biotransformation of octanoic acid into 2-heptanone by spores ofPenicillium roquefortii (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 29-35 
    Details
    ISSN: 1476-5535
    Keywords: spores ; fatty acid ; internal concentrations ; transport ; biotransformation ; Penicillium roquefortii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Internal substrate concentrations were monitored during biotransformation of octanoic acid into 2-heptanone by spores ofPenicillium roquefortii. The fatty acid rapidly enters the spores in its undissociated form, and a Collander-type relation shows that it strongly accumulates in the spore wall and membrane; this accumulation is reversible. The reaction takes place with cytoplasmic substrate concentrations that quickly fall to zero, and the process is limited by octanoic acid penetration into the cells. This entry is accompanied by proton efflux and involves an active transport process with a H+-ATPase system that exhibits Michaelian behavior. The driving force is postulated to be ΔpH, which takes a value set by the initial substrate concentration through the stoichiometry of the H+/octanoic acid exchange.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569918
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  • 10
    Electronic Resource
    Electronic Resource
    A convenient method to study fungal spores retention on a porous preformed support (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 823-829 
    Details
    ISSN: 1573-6784
    Keywords: adsorption ; Aspergillus niger ; fungal spore ; immobilization ; preformed porous carrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A method was developed to determine engineering parameters of a preformed porous cellulose carrier, commercially known as Microcube. It demonstrated a high water retention (20 g g−1 dry support) connected to a wet support density slightly higher than that of water (1.005 g ml−1), making this material suitable for use in stirred vessels; a high porosity (96%) was also evidenced. Ungerminated spores of Aspergillus niger reversibly adsorbed on the carrier according to a Langmuir-type isotherm, while germinating conidia irreversibly attached to particles, allowing a further pellet-like development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008911006199
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