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  • 1
    Electronic Resource
    Electronic Resource
    The sodium pump of erythrocytes from patients with duchenne muscular dystrophy: Effect of ouabain on the active sodium flux and on (Na+,K+)ATPase (1978)
    Springer
    Journal of neurology 217 (1978), S. 287-294 
    Details
    ISSN: 1432-1459
    Keywords: Muscular dystrophy ; Erythrocyte ; Sodium pump ; Ouabain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der aktive Ionenaustausch durch die Erythrozytenmembrane von Patienten mit Duchennescher Muskeldystrophie wurde mit folgenden zwei Methoden untersucht: — Erythrozyten-Schatten (Na+,K+)-ATPase-Aktivität und ihr Ansprechen auf Ouabain unter Anwendung einer enzymatischen Methode. — Aktives Ausströmen des Natriums und sein Ansprechen auf Ouabain unter Anwendung einer Radioisotopenmethodik. (Na+,K+)-ATPase-Aktivität und das Ausströmen des Natriums waren bei dystrophischen Patienten vermindert im Vergleich zu Kontrollfällen von gleichem Alter und Geschlecht. Die Enzymaktivität und das aktive Ausströmen von Natrium wurden durch Ouabain 10−4M bei Patienten und bei den Kontrollfällen gehemmt. Die Hemmung des aktiven Natriumausflusses war geringfügiger bei Patienten im Vergleich zu den Kontrollfällen. Die Ergebnisse wurden unter Berücksichtigung der Literatur diskutiert.
    Notes: Summary Active ion exchanges across erythrocyte membrane from patients with Duchenne muscular dystrophy were studied by two methods: — erythrocyte ghosts (Na+,K+)ATPase activity and its sensibility to ouabain, using an enzymatic method; — active sodium outflux and its sensibility to ouabain using a radioisotopic method. (Na+,K+)ATPase activity and active sodium outflux were decreased in dystrophic patients compared to normal sex- and age-matched controls. Enzymatic activity and active sodium outflux were inhibited by ouabain 10−4 M in patients and in controls. Inhibition of the active sodium outflux was lower in patients than in controls. The results are discussed in light of the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312990
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  • 2
    Electronic Resource
    Electronic Resource
    Involvement of DNA methylation in the control of the expression of an estrogen-induced breast-cancer-associated protein (pS2) in human breast cancers (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 65 (1997), S. 95-106 
    Details
    ISSN: 0730-2312
    Keywords: breast cancers ; genomic sequencing ; 5-methyldeoxycytidine ; multiple component analysis ; pS2 ; RT-PCR ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: pS2 gene has been used to investigate the relationship between alterations of DNA methylation patterns in human tumors and gene expression. The expression of pS2, which is transcriptionally controlled by estrogens in breast cancer cell lines, is restricted to estrogen-receptor-rich human breast tumors. We found that the CCGG site within the promoter/enhancer sequence of pS2 was hypomethylated in estrogen-receptor-rich breast tumors expressing this gene. The amount of DNA molecules unmethylated at this site was related to the amount of pS2 mRNA detected in the samples. The demethylation of this region, which contains the estrogen responsive element, was confirmed by genomic sequencing. Transient expression of functional human estrogen receptors stimulated the expression of the endogenous pS2 in HeLa cells, but failed, in BT-20 cells, to stimulate expression of this gene. Since the promoter/enhancer region of pS2 is unmethylated in HeLa cells and methylated in BT-20 cells, these data also support the hypothesis that DNA methylation might be involved in the control of pS2 expression. J. Cell. Biochem. 65:95-106. © 1997 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Silver stain after isoelectric focusing of unconcentrated cerebrospinal fluid: Visualization of total protein and direct immunofixation of immunoglobulin G (1982)
    Weinheim : Wiley-Blackwell
    Electrophoresis 3 (1982), S. 206-209 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A silver stain technique has been developed to study proteins in unconcentrated cerebrospinal fluid (CSF) after isoelectric focusing. This method is highly sensitive, and bands containing 25 to 50 ng protein can be clearly distinguished, so that small volumes (40 μl maximum) of native CSF can be used. Individual proteins (e. g., immunoglobulin G) can be detected easily by specific immunofixation. It is also possible to perform direct precipitation and direct specific immunofixation on a single gel in order to compare side by side the patterns of the whole and of specific proteins from different samples of CSF. The technique is simple and highly reproducible, and results are obtained 6 h after sample deposition (if immunofixation is used, a further 24 h washing is necessary). The sensitivity and versatility of this technique (immunofixation can be applied to the detection of any antigen) should permit its extension to other biological fluids with a low protein content.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150030405
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    Paper (German National Licenses)
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