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  • 1
    Electronic Resource
    Electronic Resource
    Desferrioxamine-dependent iron transport in Erwinia amylovora CFBP1430: cloning of the gene encoding the ferrioxamine receptor FoxR (1996)
    Springer
    BioMetals 9 (1996), S. 143-150 
    Details
    ISSN: 1572-8773
    Keywords: desferrioxamines ; Erwinia amylovora ; ferrioxamine receptor ; iron transport ; pathogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Iron deprivation of Erwinia amylovora CFBP1430, a species causing fire blight on Pomoïdeae, was shown to induce the production of siderophores of the desferrioxamine (dfo) family and two outer membrane polypeptides with apparent molecular weight of about 70 and 80 kDa, respectively. Cyclic dfo E was characterized as the major metabolite. Phage MudIIpR13 insertional mutagenesis and screening on CAS-agar medium yielded three dfo non-producing and one overproducing clones. These clones failed to grow in the presence of the Fe(III) chelator EDDHA and were determined further as dfo and ferrioxamine transport negative mutants, respectively. The transport mutant which appeared to lack the 70 kDa polypeptide in the outer membrane allowed the purification of dfo E. Growth under iron limitation of dfo negative mutants was stimulated with ferrioxamine E and B but not with other ferrisiderophores tested. The host DNA sequence flanking the left terminal part of the MudIIpR13 prophage responsible for the transport mutation was cloned and used to probe a parental gene library by DNA-DNA hybridization. Two recombinant cosmids restoring the transport mutation to normal were identified. Both cosmids also conferred the ability to utilize ferrioxamine B and E as iron sources on a FhuE1 mutant of Escherichia coli. This correlated with the production of an additional polypeptide of 70 kDa in the outer membrane of E. coli transconjugants, thus confirming that this protein serves the ferrioxamine receptor function (FoxR) in E. amylovora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144619
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural study ofErwinia amylovora strains: Effect of culture conditions and fixation procedures (1987)
    Springer
    Protoplasma 139 (1987), S. 1-8 
    Details
    ISSN: 1615-6102
    Keywords: Ultrastructure ; Erwinia amylovora ; Outer membrane ; Fixation procedure ; Cold storage ; Virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Modifications of the ultrastructure of the plant pathogenic bacteriumErwinia amylovora were analyzed according to growth conditions and fixation procedures. Six bacterial strains with various virulence characteristics were examined. Cultures were grown either in Yeast Peptone Glucose medium (YPG) or in a medium containing asparagine (ASP) supplemented with sorbitol (1% or 5% sorbitol). When grown in ASP + 1% sorbitol or in YPG, the strains, structurally similar to each other in ASP + 5% sorbitol, presented different frequencies of small evaginations which were observed arising from the cell surface mainly after an OsO4 fixation step. There was no correlation between the frequency of evaginations and the virulence of the strain. An overnight storage at 4 °C considerably enhanced the frequency of the evaginations. It was suggested that the OsO4 fixation step visualized differences in the bacterial outer membrane structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01417529
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mutations in ams genes of Erwinia amylovora affect the interactions with host plants (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 313-322 
    Details
    ISSN: 1573-8469
    Keywords: bacterial pathogenicity ; exopolysaccharide ; fire blight ; host-pathogen interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A large region for exopolysaccharide (EPS) production that is involved in the pathogenicity of Erwinia amylovora strain CFBP1430, cloned previously, was further characterized in this study. An 8.4 Kb-long sub-cloned fragment from that ams (amylovoran synthesis) region was mutagenized in vitro by Tn3Gus insertion. Several new chromosomal mutants were obtained by marker exchange. The phenotype of those mutants was determined with regard to pathogenicity, EPS production, and growth in planta. It appeared that alteration in EPS synthesis was correlated with altered pathogenicity and poor (or no) growth in planta. Altogether these data argue for a direct role of EPS in bacterial multiplication in planta, and/or for an indirect role by protecting the bacteria against host defence reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008693614060
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    Paper (German National Licenses)
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