ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The circulating monocyte exhibits the capacity to initiate and accelerate the coagulation cascade. We have devised a simple whole blood clotting assay which quantitates the monocyte's contribution of procoagulant (a marker of monocyte activation) to the clotting process and in addition, measures thein vivo activation of the cell. Citrated whole blood is added to saline and the recalcification time (RT) determined without incubation (RT control), with two hours incubation (RT saline), or added to endotoxin with incubation (RT endotoxin). The reduction in value between RT control and RT saline is a measure of monocyte activationin vivo. The reduction, in time between RT saline and RT endotoxin is a marker of monocyte activationin vitro. This simple test enables the measurement of monocyte activation and this cells contribution to the hypercoagulability described in many disease states.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01993228
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