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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of wheat with high molecular weight subunit genes results in improved functional properties (1997)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 15 (1997), S. 1295-1299 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The high molecular weight (HMW) subunits of wheat glutenin are major determinants of the elastic properties of gluten that allow the use of wheat doughs to make bread, pasta, and a range of other foods. There are both quantitative and qualitative effects of HMW subunits on the quality of the grain, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1197-1295
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  • 2
    Electronic Resource
    Electronic Resource
    Plant regeneration of wildGlycine species from suspension culture-derived protoplasts (1989)
    Springer
    Plant cell reports 8 (1989), S. 112-115 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts isolated from four-week old cell suspension cultures ofGlycine canescens F. J. Herm andG. clandestina Wendl. were cultured in 8P or modified 8P to a multicellular stage. Colonies of 0.5 to 1.0 mm diameter were transferred to solid media for callus growth and regeneration. Callus consisted of friable masses with compact green nodular areas. Organogenesis of both species occurred primarily from the green nodular areas. Shoot buds ofG. clandestina did not mature, but shoots ofG. canescens proliferated on MS medium, with B5 vitamins, 0.33 mgL−1 each BA, KN, ZN, and 0.15 mgL−1 NAA. Shoots failed to root after multiple subcultures on four different rooting media.In vitro grafting ofG. canescens scions ontoG. max root stocks allowed plants to be transferred to soil. An overall protoplast division efficiency of 48% was achieved with moderately efficient shoot regeneration inG. canescens. Division efficiencies forG. clandestina were lower (11%). Refinements of this protocol should result in high efficiencies of regeneration which would allowin vitro manipulations of these wild soybean relatives at the single cell level and would make the derivation of somatic hybrid plants possible within the genusGlycine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716852
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.) (1987)
    Springer
    Plant cell reports 6 (1987), S. 305-308 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272005
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  • 4
    Electronic Resource
    Electronic Resource
    Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum (1999)
    Springer
    Euphytica 108 (1999), S. 161-167 
    Details
    ISSN: 1573-5060
    Keywords: barley ; embryogenesis ; medium ; regeneration ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Media have been developed for somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. For wheat and tritordeum inflorescences, regeneration from embryogenic calluses induced on medium with picloram was almost twice as efficient as regeneration from cultures induced on 2,4-dichlorophenoxyacetic acid (2,4-D). The addition of zeatin at 5 or 10 mg l−1 to regeneration media had a positive effect on regeneration. For scutella, the highest frequencies of embryogenesis (85%) and regeneration (50%) was obtained using an induction medium containing 2 mg l−1 of 2,4-D and half concentration of aminoacids. The morphogenetic capacities of 19 different cultivars of wheat, barley and tritordeum were compared, and clear differences were found both between explants and genotypes. In wheat, embryogenic capacity from inflorescences (average of 92%) was higher than from immature scutella (average of 62%). However, shoot regeneration from scutella was clearly higher than from inflorescences (averages of 63%, and 18% respectively). Frequencies of regeneration in wheat and barley varied widely among the cultivars tested and in both species no difference was found between spring and winter varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003676830857
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  • 5
    Electronic Resource
    Electronic Resource
    Soybean somatic embryogenesis: Effects of hormones and culture manipulations (1987)
    Springer
    Plant cell, tissue and organ culture 10 (1987), S. 197-208 
    Details
    ISSN: 1573-5044
    Keywords: soybean ; somatic embryogenesis ; hormones ; tissue culture ; embryo morphology ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryos were induced in cultures of immature soybean (Glycine max (L.) Merr) embryos, or isolated cotyledons on MS modified medium supplemented with NAA and 2,4-D, BAP and ABA. When NAA and 2,4-D were compared at similar concentrations (25 and 23 μM), 2,4-D produced larger number of somatic embryos, however, embryogenesis efficiency was improved in media containing NAA by using higher levels (100–150 μM) of the auxin. Somatic embryo morphology varied with auxin type: NAA-induced embryos more closely resembled zygotic embryos than did 2,4-D-induced embryos. Additions of BAP or ABA to auxin-containing media had either no effect or reduced embryo production, although ABA altered the morphology of 2,4-D-induced embryos. In media containing both NAA and 2,4-D, the latter was dominant in terms of embryo morphology. The effects of subculture frequency and of transfers between 2,4-D and NAA media were investigated: Subculture frequency influenced mainly the frequency of normal embryos, while preculture on 2,4-D increased subsequent embryogenesis efficiency on NAA medium but reduced the frequency of normal embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037304
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  • 6
    Electronic Resource
    Electronic Resource
    Soybean somatic embryogenesis: Effects of nutritional, physical and chemical factors (1987)
    Springer
    Plant cell, tissue and organ culture 10 (1987), S. 209-220 
    Details
    ISSN: 1573-5044
    Keywords: soybean ; tissue culture ; somatic embryogenesis ; nutritional and physical factors ; plant regeneration ; plantlet growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immature soybean (Glycine max (L.) Merr) embryos, or cotyledons isolated from them, were cultured on modified MS medium containing B5 vitamins and NAA (50 μM) to induce somatic embryogenesis. The effects of media variables, dissection treatments and light conditions were investigated in this system. The efficiency of embryogenesis increased as sugar concentration decreased from 12 to 1.5%; sucrose and glucose were similarly effective as carbon sources. In an examination of the effects of medium pH, values between pH 5.0 and 7.0 gave similar embryogenesis efficiencies, but the frequency of normal embryos was greater in media with low pH values. In buffered medium (10 mM MES), a pH of 5.0 was inhibitory to embryogenesis, and most normal embryos were produced at pH 5.5. Under various dissection treatments, embryogenesis efficiency and root and callus production were increased by tissue damage. Somatic embryogenesis was observed both in darkness and in light, although embryo development was impaired under high light (80 μE m-2 s-1) conditions. The ability of somatic embryos to germinate was closely correlated with embryo normality, and was influenced little by the hormone content of germination media. Of various media tested for their ability to support the growth of germinated embryos, a medium based on hydroponic nutrient salts, supplemented with yeast extract, and gelled with Difco-Bacto agar gave the best plantlet growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037305
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of enzyme solution on protoplast culture and transient gene expression in maize (Zea mays L.) (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 43-48 
    Details
    ISSN: 1573-5044
    Keywords: β-glucuronidase ; Macerozyme ; plating efficiency ; protoplast yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of two enzyme solutions, differing only in the presence or absence of Macerozyme, on protoplast yield, colony formation and transient GUS (β-glucuronidase) activity was studied. For all parameters tested the presence of Macerozyme during protoplast isolation had a negative influence. Using an enzyme solution without Macerozyme suspension aggregates gave up to 4.4 times higher protoplast yield and plating efficiencies were increased up to 10-fold. Further, protoplasts isolated without macerozyme showed a 5.2-fold higher GUS activity in transient gene expression. Apart from the presence of Macerozyme, longer incubation (3 compared with 1.5 h) of cell aggregates in the enzyme solution also had a negative effect on transient transformation efficiency. These data demonstrate that protoplast isolation conditions have a profound effect on transient gene expression and it is proposed that these factors will also influence stable transformation efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037590
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