ISSN:
1573-3904
Keywords:
Allergic contact dermatitis
;
Antigenic peptide
;
Fmoc chemistry
;
Solid-phase synthesis
;
Synthetic peptide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract N-α-Fmoc-N-τ-methyl-L-histidine was prepared in three stepsfrom N-α-Boc-L-histidine by treatment with methyliodine in DMF at–10 °C, deprotection of the N-α position in pure TFA andsubsequent reprotection by Fmoc-chloroformate in a 5%Na2CO3/dioxane mixture. N-α-Fmoc-N-τ-methyl-L-histidine was then used for the solid-phase synthesis oftwo analogues of the OVA323-336 T-epitope, methylated onHis331 and on His328/331, respectively. Thesepeptides were tested for their ability to activate 3 D0-54.8 T-cellhybridoma when presented by fixed A-20.1.11 antigen presenting cells, and nosignificant difference was o7 bserved in IL-2 production.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008841328236
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