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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the Soluble and Membrane-bound Immobilization Antigens of Ichthyophthirius multifiliis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 42 (1995), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . The immobilization antigens (i-antigens) are a class of highly abundant surface membrane proteins found on a number of holotrich ciliates. In Ichthyophthirius multifiliis (an obligate parasite of fish) these antigens appear to be targets of the host immune response. While the i-antigens of Ichthyophthirius are predominantly membrane-associated proteins, we now find that they are released into the water surrounding the parasite in a highly enriched form. The membrane-associated and water soluble proteins appear indistinguishable by antigenic means, as well as by several biochemical criteria including peptide mapping, mobility in reducing and non-reducing SDS-polyacrylamide gels, and relative glycosylation. Antibodies raised against the membrane-associated antigens react with the water soluble proteins on Western blots. Not surprisingly, immunocytochemical localization studies show binding of these antibodies to surface membranes of the cell. In addition, however, antibody binding is also detectible on the membranes of a secretory organelle (that is, mucocysts) present in the cortical cytoplasm. The significance of these findings with regard to the potential role of the i-antigens in infection and immunity is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1995.tb05905.x
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  • 2
    Electronic Resource
    Electronic Resource
    Serotypic Variation Among Isolates of Ichthyophthirius multifiliis Based on Immobilization (1993)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 40 (1993), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Efforts have been made to determine whether surface antigens could be used as biochemical markers to define strain differences in the parasitic ciliate Ichthyophthirius multifiliis. In previous studies, a wild-type isolate designated G1 was found to have surface proteins analogous to the immobilization antigens of Paramecium and Tetrahymena; rabbit antiserum against this strain immobilizes homologous cells in vitro. It has now been shown for two additional Ichthyophthirius isolates (designated G1.1 and G2) that immobilization antigens are both present and serologically distinct. Proteins of similar size, which cross-react in Western blots with rabbit antisera against immobilization antigens of the G1 strain, are nevertheless found in the G1.1 and G2 isolates. As shown by Southern blotting analysis, the G1.1 and G2 strains also contain genomic DNA sequences which hybridize with an immobilization antigen cDNA from G1 when probed under conditions of reduced stringency. The serotypic differences in immobilization between I. multifiliis isolates appear to be stable over time and provide a means of discriminating strains. In addition to providing a basis for comparative studies, the work described here has implications for the development of vaccines against this important fish parasite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1993.tb04480.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Seasonal changes in planktonic bacterial assemblages of two Ohio streams (1998)
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 39 (1998), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. Monthly water samples were collected from two Ohio streams (Bixon and Mahoning) for 14 months to assess the extent of seasonal changes in the bacterial assemblage and in a population within the assemblage. At the assemblage level, abundances of total bacteria and colony forming units (CFUs) were measured. Populations of Burkholderia (Pseudomonas) cepacia were quantified by colony hybridization using a species-specific rDNA probe.2. Total bacterial numbers were highest in early winter and were lowest during summer and when the streams were covered with ice. Changes in the number of CFUs were more variable than changes in total number, but did not exhibit a clear seasonal pattern.3. B. cepacia was not detected during summer, but up to 8000 ml–1 were present in November. Seasonal changes in assemblage-level measurements were dissimilar to population-level changes in B. cepacia abundance.4. The temporal changes observed in this study suggest that populations of stream bacteria, such as B. cepacia, exhibit seasonal blooms that are undetected by assemblage level measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2427.1998.00269.x
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