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  • 1
    Electronic Resource
    Electronic Resource
    Cisplatin induces the pro-apoptotic conformation of bak in a delta-MEKK1-dependent manner (2001)
    [s.l.] : Nature Publishing Group
    Nature genetics 27 (2001), S. 86-86 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Cisplatin induces the pro-apoptotic conformation of the Bcl-2 family protein Bak in four out of four human melanoma cell lines, whereas an effect on the pro-apoptotic conformation of Bax was seen in only one (FM55 cells). Expression of a kinase-inactive fragment of MEKK1 (dnMEKK) efficiently ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/87292
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  • 2
    Electronic Resource
    Electronic Resource
    Classification of human ovarian tumors using multivariate data analysis of polypeptide expression patterns (2001)
    [s.l.] : Nature Publishing Group
    Nature genetics 27 (2001), S. 68-68 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Quantitative molecular variation may be used for the development of methods for tumor classification. We used the statistical concept of principal component analysis to type ovarian tumors. We purified tumor cells from ovarian tumors and subjected them to two-dimensional gel electrophoresis. Using ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/87176
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of Mechanisms and Frequency of CDKN2A/B Gene Loss During Progression of RAS-Transformed Rat Embryo Fibroblast Clones (1998)
    Springer
    Somatic cell and molecular genetics 24 (1998), S. 327-339 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Rat embryo fibroblasts (REFs) are inefficiently transformed by RAS-oncogenes. Induction of p16 INK4A expression by RAS has been suggested to contribute to this resistance. Glucocorti-coid hormones, (DEX), enhance REF transformation by RAS and facilitates the isolation of transformed and immortal cell lines. We show that DEX induced cell proliferation is paralleled by a decrease in Cdkn2a gene transcripts, suggesting a mechanism for hormone promotion. The mechanisms of progression into hormone independent cell lines were examined. Twenty-two of 30 clones which reached a population size of approximately 10 6 cells could be established as cell lines. All lines studied showed homozygous deletions of the Cdkn2 loci (Cdkn2a and Cdkn2b) on RNO5. LOH was found for all RNO5 genetic markers examined in 7 of 19 cell lines, suggesting non-disjunction events. In the remaining 12 cell lines, both copies of Cdkn2 appeared to be lost by deletions/rearrangements, some of which could by demonstrated by karyotype analysis. We conclude that (i) clonal expansion of RAS-transfected REF by DEX is paralleled by down-regulation of Cdkn2a expression; (ii) homozygous deletion of Cdkn2 were estimated to occur at a frequency of 2 × 10 −8 /cell/generation or higher, and (iii) deletion/rearrangements and non-disjunction appear to be the main mechanisms leading to deletion of Cdkn2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1024486307061
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  • 4
    Electronic Resource
    Electronic Resource
    Repression of stromelysin metalloprotease expression in rat fibrosarcoma cells by dimethylsulfoxide (1993)
    Springer
    Clinical & experimental metastasis 11 (1993), S. 77-82 
    Details
    ISSN: 1573-7276
    Keywords: dimethylsulfoxide ; invasion ; metalloprotease ; stromelysin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Metalloproteases are implicated in conferring invasive properties to tumor cells. We show here that treatment of ras-oncogene-transformed rat fibroblasts with dimethylsulfoxide (DMSO) results in a reversible decrease in stromelysin mRNA. Furthermore, stromelysin expression was found to be repressed by DMSO, but not by glucocorticoid hormone, in a fibrosarcoma cell line showing low AP-1 (fos/jun) transcription factor activity. In two fibrosarcoma cell lines which express high levels of stromelysin and low levels of 68 kDa type IV collagenase, the DMSO-induced decrease in stromelysin expression was paralleled by a decreased invasive propensity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00880068
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution of stromelysin-3 mRNA transcripts and microvessels in human breast carcinomas (1997)
    Springer
    Breast cancer research and treatment 42 (1997), S. 207-213 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; matrix metalloproteases ; stroma ; tumor angiogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Several proteinases, implicated in tumor invasion, are expressed in fibroblastic cells surrounding neoplastic cells, and are believed to be induced by paracrine stimulation. Such stimulation, by the local release of angiogenic factors, is also responsible for the induction of new capillary blood vessels, a crucial aspect of tumor growth and metastasis. We have here compared the expression of a matrix metalloproteinase, stromelysin-3 (ST3), with the distribution of tumor microvessels in invasive breast carcinomas. The highest level of ST3 mRNA in each tumor was recorded and compared to the highest microvessel density. No correlation between these two parameters was observed by analysis of 63 tumors. Detailed examination of 19 individual tumors did not reveal any correlation between the distribution of ST3 mRNA and microvessels. In the material studied here, ST3 expression was observed to correlate with long-term survival of the patients, whereas microvessel density did not correlate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005769622570
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  • 6
    Electronic Resource
    Electronic Resource
    ERK signalling in metastatic human MDA-MB-231 breast carcinoma cells is adapted to obtain high urokinase expression and rapid cell proliferation (1999)
    Springer
    Clinical & experimental metastasis 17 (1999), S. 649-654 
    Details
    ISSN: 1573-7276
    Keywords: u-PA ; cyclin D1 ; breast cancer ; ERK
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Increased urokinase plasminogen activator (u-PA) production is associated with tumor invasion and metastasis in several malignancies, including breast cancer. The mechanisms underlying constitutive u-PA expression are not well understood. We examined the relationship between the signal strength of the ERK pathway and the level of u-PA expression in the metastatic human breast cancer cell line MDA-MB-231. Treatment with the MEK1 inhibitor PD98059 resulted in decreased ERK1/2 phosphorylation and decreased u-PA mRNA and protein expression. Inhibition of ERK1/2 activity also led to decreased cell proliferation and to decreased cyclin D1 expression. Less than 5% of total ERK1/2 was phosphorylated in exponentially growing MDA-MB-231 cells, and ERK1/2 activity could be stimulated by okadaic acid. Okadaic acid did not stimulate u-PA expression, but induced strong expression of the cdk-inhibitor p21Cip1. These findings suggest that ERK1/2 signaling is tuned to a level which results in high u-PA expression and rapid cell proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006741228402
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of tissue and serum VEGF in patients with head and neck carcinoma (1998)
    Springer
    Angiogenesis 2 (1998), S. 365-372 
    Details
    ISSN: 1573-7209
    Keywords: head and neck carcinoma ; tumor marker ; VEGF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Serum vascular endothelial growth factor (VEGF) was measured in 54 cancer patients with head and neck carcinoma. In addition, tumor VEGF was examined by immunohistochemistry in sections of biopsies obtained within 4 weeks to serum sampling in 37 of these patients. Serum VEGF levels were higher in the sera of the tumor patients than in the sera of healthy control subjects (P 〈 0.005). Patients with stage II–IV tumors showed increased levels of serum VEGF, whereas patients with stage I tumors did not. The receiver operating characteristics (ROC) of serum VEGF were similar to those observed with TPS (tissue protein specific antigen). Immunohistochemistry of tissue sections showed that 24/37 tumors were VEGF positive. No connection was observed between strong VEGF staining of tumor tissue sections and high levels of serum VEGF. We conclude that serum VEGF could be a useful marker for monitoring head and neck carcinoma patients, but that serum and tissue VEGF levels do not appear to correlate with each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009211605162
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  • 8
    Electronic Resource
    Electronic Resource
    Transcription of chick genes by mammalian RNA polymerase II in chick erythrocyte-mammalian cell heterokaryons (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 113 (1982), S. 99-104 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The introduction of chick erythrocyte nuclei into mammalian cell cytoplasms results in their reactivation as evidenced by the de novo transcription of chick genes and the synthesis of both globin and constitutive proteins. In the present study, chick erythrocytes have been fused to L6 rat myoblasts and to alphaamanitin-resistant variants of L6 to determine whether the chick or the mammalian RNA polymerase II was responsible for transcription of chick genes. Heterokaryons formed by fusing chick erythrocytes with alpha-amanitin-resistant L6 myoblasts synthesize both chick globin and chick constitutive proteins in the continued presence of 5 μg/ml alpha amanitin ten days postfusion. Both the synthesis of globin and other chick polypeptides occurs at levels comparable to those observed for untreated heterokaryons. Synthesis occurs under conditions in which insignificant chick RNA polymerase II activity can be detected irv wild-type heterokaryons by autoradiography. These results demonstrate that RNA polymerase II is one of the mammalian proteins that is selectively taken up by the chick nucleus during reactivation in the presence of alpha amanitin. Furthermore, the mammalian RNA polymerase II alone can account for the transcription of both differentiation specific and constitutive genes in the chick nucleus.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041130117
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  • 9
    Electronic Resource
    Electronic Resource
    Induction of the collagenase phorbol ester response element by staurosporine (1994)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 55 (1994), S. 496-502 
    Details
    ISSN: 0730-2312
    Keywords: staurosporine ; protein kinases ; collagenase ; AP-1 ; gene induction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The indol alkaloid staurosporine is a potent inhibitor of protein kinase C, but has also been shown to have certain effects paradoxically similar to those of protein kinase C-activating phorbol esters. We show here that collagenase mRNA expression is stimulated by 10 nM staurosporine in normal and ras-oncogene-transformed rat fibroblasts. The kinetics of collagenase mRNA induction by staurosporine were slow compared to induction by phorbol ester. Staurosporine induction of the collagenase promoter appeared to be mediated via the TPA response element (TRE). Induction did not involve any increase in jun mRNA expression and did not require expression of c-Jun. Prolonged treatment with phorbol ester to deplete protein kinase C did not inhibit stimulation of the collagenase promoter by staurosporine. Instead, involvement of cAMP-dependent protein kinase (PKA) was indicated by inhibition of staurosporine induction by the PKA inhibitor H-89. In addition, raised levels of cAMP were observed during the first hour of staurosporine treatment. Altogether, our data indicate that staurosporine induces a PKA-dependent pathway leading to c-Jun-independent activation of the collagenase TRE element. © 1994 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240550409
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  • 10
    Electronic Resource
    Electronic Resource
    Rapid isolation of phage displayed antibodies to β-actin eluted from two-dimensional electrophoresis gel (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1808-1810 
    Details
    ISSN: 0173-0835
    Keywords: Phage display antibody ; Two-dimensional polyacrylamide gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We describe a simple and efficient procedure which can be used to prepare antibodies to proteins extracted by two-dimensional gel electrophoresis (2-DE), using β-actin as a model. Protein was electroeluted from a stained gel, biotinylated and used for selection of phage from a semisynthetic phage antibody library. After four rounds of selection using 50 ng β-actin per cycle, approximately 8 × 103 phage were recovered. Antibody fragments were prepared from 21 randomly picked clones. Six of eighteen (6/18) antibody-positive clones produced antibody fragments reacting against β-actin in an enzyme linked immunosorbent assay (ELISA). Sequencing of the HC-CDR3-region showed that all six clones were independent isolates, suggesting that a large number of independent phage antibody reactivities were generated.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191044
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