ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Plant Regeneration from Protoplasts in Linum (1987)

Ling, H. Q. ; Binding, H.

Oxford, UK : Blackwell Publishing Ltd

Plant breeding 98 (1987), S. 0

add to mindlist on the mindlist

Details

ISSN: 1439-0523

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Protoplasts of eight wild Linum species and 14 genotypes of flax, isolated from different parts of seedlings and from shoot apices, were regenerated to callus in medium V-KM (Binding and Nehls 1977). Plant regeneration was obtained in L. alpinum, L. amurense, L. hologynum, L. perenne, L. salsoloides and eight genotypes of L. usitatissimum on agar media B5 (Gamborg et al. 1968), B5C (Binding et al, 1981), MS (Murashige and Skoog 1962) and MSC (MS with 5% coconut endosperm) containing 2.5μM 6-BA. An adventitious embryo was regenerated in L. alpinum. Callus differentiation like sponge parenchyma, was observed in protoplast-derived tissues of L. usitatissimum. Shoots organized roots under slightly divergent conditions in the different species.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01135.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.) (1998)

Ling, H.-Q. ; Kriseleit, D. ; Ganal, M. W.

Springer

Plant cell reports 17 (1998), S. 843-847

add to mindlist on the mindlist

Details

ISSN: 1432-203X

Keywords: Key wordsLycopersicon esculentum ; Agrobacterium tumefaciens ; Transformation ; Cefotaxime ; Ticarcillin/potassium clavulanate

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Ticarcillin/potassium clavulanate is a very effective combination of antibiotics to eliminate Agrobacterium tumefaciens during tomato transformation. It shows no toxicity to tomato tissues at a concentration of 150 mg/l and significantly promotes callus formation and shoot regeneration. The transformation frequency was raised more than 40% in comparison to cefotaxime. Cefotaxime itself did not inhibit callus growth in culture medium, but it clearly decreased shoot differentiation. Together with kanamycin, cefotaxime shows a strong negative effect on callus growth, shoot regeneration and transformation efficiency. Unlike the widely used carbenicillin and cefotaxime, ticarcillin/potassium clavulanate is light stable and resistant to inactivation by β-lactamase. Furthermore, ticarcillin/potassium clavulanate is more economical than carbenicillin and cefotaxime. In conclusion, ticarcillin/potassium clavulanate is a very good alternative to eliminate Agrobacterium tumefaciens in plant transformation and has the potential to be widely used for plants which are sensitive to carbenicillin and cefotaxime.