ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Polyurethane foam and nylon-web carriers were compared for simultaneous production of endo-1, 4-β-glucanase and xylanase by immobilized Trichoderma reesei on a medium based on lactose (27 g/l), cellulose (3 g/l) and sorbose (0.3 g/l). Nylon sheet with 1.2 cm2 carrier surface/ml medium was superior to the others, and it was selected for further studies. The carbon source had a marked effect on enzyme production by the immobilized fungus. With pure cellulose (10 g/l) as substrate, the maximum endoglucanase activity was 690 nkat/ml and xylanase activity 4800 nkat/ml. Supplementation with 0.5 g/l of sorbose resulted in an increase in both endoglucanase and xylanase activities in all media studied. A more detailed study on the effect of sorbose on a lactose(7 g/l)- and cellulose(3 g/l)-based medium revealed a clear optimum sorbose concentration of 1.5 g/l, with a maximum endoglucanase activity of 660 nkat/ml, xylanase activity of 3670 nkat/ml, and filter-paper activity (overall cellulolytic activity) of 2.0 filter-paper units/ml. However, the addition of 1.5 g/l sorbose to the pure-cellulose(10 g/l)-based medium resulted in a slight decrease in the enzyme production.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002530050490
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