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  • 1
    Electronic Resource
    Electronic Resource
    Corticotrophin-releasing hormone and corticotrophin- releasing hormone binding protein in normal and pre-eclamptic human pregnancies (1995)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 102 (1995), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To measure the plasma levels of corticotrophin-releasing hormone and corticotrophin-releasing hormone binding protein in normal pregnancy and in pregnancies complicated by pre-eclampsia.Setting John Radcliffe Hospital, Oxford and St Thomas's Hospital, London.Subjects One hundred and twenty pregnant women sampled prospectively throughout gestation, of whom 91 experienced a normal pregnancy and eight developed pre-eclampsia; in a second study, 10 women with severe pre-eclampsia, presenting at a range of gestational ages, were sampled once and compared with appropriately matched normal pregnant women.Main outcome measure Plasma levels of corticotrophin-releasing hormone determined by immunoradiometric assay. Plasma levels of corticotrophin-releasing hormone binding protein measured by direct radioimmunoassay.Results In the prospective study, plasma samples from women with pre-eclampsia exhibited higher (390.2 versus 292.7 pmol/l at 36 weeks) levels of corticotrophin-releasing hormone and significantly lower (5.24 versus 8.14 nmol/l at 36 weeks, P 0.002) levels of corticotrophin-releasing hormone binding protein than normal controls. In the second, single time point study a significant elevation in CRH (P 〈 0.002) and reduction in CRH-BP (P 〈 0.001) was found in pre-eclamptic pregnancies compared with controls.Conclusions In human pregnancies complicated by pre-eclampsia there is an elevated level of corticotrophin releasing hormone whilst there is less corticotrophin-releasing hormone binding protein; therefore there is a net increase in free potentially bioactive hormone which may play a role in the pathology of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1995.tb09063.x
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  • 2
    Electronic Resource
    Electronic Resource
    Plasma corticotrophin-releasing factor (CRF) in abnormal pregnancy (1988)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 95 (1988), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Maternal plasma levels of cortiocotrophin-releasing factor (CRF) have been measured in abnormal pregnancy states to assess their potential as biochemical markers for at-risk pregnancies. CRF levels were not significantly altered in patients with hydatidiform mole, polyhydramnios or diabetes. CRF levels were elevated in pregnancies complicated by accidental antepartum haemorrhage at 28 weeks (P〈0·03) but not for the rest of the third trimester. In twin pregnancies CRF levels were significantly raised throughout the third trimester (28–32 weeks, P〈0·01; 34–36 weeks, P〈0·001). In patients with pregnancy-induced hypertension (28 weeks, P〈0·001; 32–36 weeks, P〈0·001; and 38–40 weeks, P〈0·01), preterm labour and premature rupture of the membranes (28 weeks, P〈0·004; 30–32 weeks, P〈0·002; and 34–36 weeks, P〈0·001), CRF levels were significantly raised and in some patients levels were elevated 11 weeks before the onset of signs or symptoms. These observations raise the possibility that maternal CRF measurement may be of use as a predictive indicator of certain at-risk pregnancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1988.tb06504.x
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  • 3
    Electronic Resource
    Electronic Resource
    The rate of rise of corticotrophin releasing factor and endogenous digoxin-like immunoreactivity in normal and abnormal pregnancy (1990)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 97 (1990), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Maternal plasma concentrations of corticotrophin releasing factor (CRF) and endogenous digoxin-like immunoreactivity (EDLI) were estimated in 80 normal and 88 abnormal pregnancies which were sampled sequentially from 24 weeks gestation to delivery. A slope was fitted for each woman's antenatal EDLI and CRF values, both of which rose significantly during gestation, and the mean of the slopes for the normal and abnormal groups for each value compared. There was no evidence of significant mean differences between groups for EDLI but there was evidence of a significant mean difference for CRF (P〈0.05). After adjustment for other variables which may affect pregnancy outcome, the slopes for CRF were found not to be significantly related to outcome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1990.tb02579.x
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  • 4
    Electronic Resource
    Electronic Resource
    Plasma corticotrophin-releasing factor (CRF) in normal pregnancy (1988)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 95 (1988), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Corticotrophin-releasing factor (CRF) was measured directly in maternal plasma using an immunoradiometric assay (IRMA). In the first and second trimester CRF levels were within the non-pregnant range (mean 15 pg/ml). A total of 72 women was followed sequentially from 28 weeks until delivery and CRF levels rose from a median of 20 pg/ml at 28 weeks to 1320 pg/ml at 40 weeks and 1732 pg/ml during labour. There was a strong correlation (rs= 0·81, P〈0·001) between gestational age and CRF levels. The rate of rise of CRF (pg/ml) per week was associated with weight gain (rs= 0·36, P〈0·05) but with no other obstetric variable. There was an association between umbilical cord and maternal plasma CRF levels (rs= 0·54, P〈0·01).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1988.tb06503.x
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  • 5
    Electronic Resource
    Electronic Resource
    Corticotrophin-Releasing Hormone Receptor Type 1: Generation and Characterization of Polyclonal Antipeptide Antibodies and their Localization in Pituitary Cells and Cortical Neurones in vitro (1996)
    Oxford : Blackwell Science Ltd.
    Journal of neuroendocrinology 8 (1996), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Corticotrophin-releasing hormone (CRH) is a 41 amino acid neuropeptide which plays a major role in regulating the endocrine response to stress. CRH acts by first binding to specific receptors on the plasma membrane of target cells. A CRH receptor from a human corticotroph adenoma and rat brain has recently been cloned (CRH-R1). In this paper, we have chosen three different peptide sequences within the CRH-R1 molecule which bear no similarity to other members of this receptor subfamily (or indeed any known protein) and which are likely to be exposed on the surface of the native protein, for antibody production. Some of these fragments produced anti-peptide antibodies of good titre which cross-reacted with the CRH-R1 receptor expressed in transiently transfected COS-7 cells and in tissue extracts from rat cerebellum, cortex, pituitary gland and human myometrium, both in Western blots and in liquid-phase radioimmunoassay. We used immunofluorescence techniques to localize the CRH receptor in transiently transfected COS-7 cells, primary cultures of rat anterior pituitary (AP) cells, the corticotroph-tumour cells AtT20 D16–16 and cortical neurons in primary culture. Our results indicate IR-CRH-R1 receptors have a punctate distribution on the plasma membrane of AP cells and AtT20 D16–16 cells. Whilst in AP cells their appearance is a fine punctate pattern, in AtT20 cells, they appear as large patches which could account for receptor clusters. Within primary cortical neurons, their distribution does not appear to be polarized. Our results suggest that distribution of CRH-R1 receptors within the different cell-types investigated depends not only on the amino acid sequence but also on cellular factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2826.1996.04866.x
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  • 6
    Electronic Resource
    Electronic Resource
    Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin (1982)
    [s.l.] : Nature Publishing Group
    Nature 299 (1982), S. 355-357 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The bioassay used in this study was that described previously12, except that the antioxidant, ascorbic acid, was added to all physiological media (60 jxg ml"1) and peptide solutions under storage (1 mg ml"1) for two reasons. First, we have observed synergism between chromatographically separated ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/299355a0
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins (1991)
    [s.l.] : Nature Publishing Group
    Nature 349 (1991), S. 423-426 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Human CRF-binding protein (hCRF-BP) was purified as previously described19 and subjected to SDS gel electrophoresis. The 37K band (relative molecular mass (Mr) 37,000) was tryp-sinized and the resultant fragments separated on reversed-phase HPLC and sequenced by Edman degradation. Sequence ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/349423a0
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  • 8
    Electronic Resource
    Electronic Resource
    Pro- γ -melanocyte-stimulating hormone cleavage in adrenal gland undergoing compensatory growth (1983)
    [s.l.] : Nature Publishing Group
    Nature 306 (1983), S. 70-73 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Dramatic and highly significant increases in adrenal weight, RNA and DNA are seen in the remaining adrenal gland 24 h after unilateral adrenalectomy compared with sham-operated controls (Fig. 1), and provide us with a rapid but objective assessment of cellular hypertrophy (RNA) and hyperplasia ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/306070a0
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  • 9
    Electronic Resource
    Electronic Resource
    Co-localisation of autoimmune antibodies specific for double stranded DNA with procorticotrophin-releasing hormone within the nucleus of stably transfected CHO-K1 cells (1995)
    Springer
    Cell & tissue research 282 (1995), S. 367-376 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Autoimmune antibodies ; Corticotrophin-releasing hormone ; Double stranded DNA ; Nucleus ; Cell culture ; CHO-K1 cells (Chinese Hamster Ovary)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Human autoantibodies and corticotrophin-releasing hormone (CRH)-specific antibodies have been used in a double-labelling immunofluorescence technique to demonstrate that immunoreactive CRH structures are co-localised with immunostaining produced by double stranded DNA-specific human autoantibodies within the nucleus of cultured ovarian cells of Chinese hamsters (CHO-K1). This co-localisation was confirmed using confocal microscopy. A metabolic labelling technique was used to investigate the role of the cytoskeleton in mediating nuclear translocation of proCRH within stably transfected CHO-K1 cells and showed that microtubule and actin disrupting agents had no effect upon the nuclear translocation of proCRH. These results, therefore, suggest that nuclear translocation of proCRH is not affected by drugs which disrupt the cytoskeleton and, consequently, modify the diameter of the nuclear pores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050488
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  • 10
    Electronic Resource
    Electronic Resource
    Co-localisation of autoimmune antibodies specific for double stranded DNA with procorticotrophin-releasing hormone within the nucleus of stably transfected CHO-K1 cells (1995)
    Springer
    Cell & tissue research 282 (1995), S. 367-376 
    Details
    ISSN: 1432-0878
    Keywords: Autoimmune antibodies ; Corticotrophin-releasing hormone ; Double stranded DNA ; Nucleus ; Cell culture ; CHO-K1 cells (Chinese Hamster Ovary)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human autoantibodies and corticotrophin-releasing hormone (CRH)-specific antibodies have been used in a double-labelling immunofluorescence technique to demonstrate that immunoreactive CRH structures are co-localised with immunostaining produced by double stranded DNA-specific human autoantibodies within the nucleus of cultured ovarian cells of Chinese hamsters (CHO-K1). This co-localisation was confirmed using confocal microscopy. A metabolic labelling technique was used to investigate the role of the cytoskeleton in mediating nuclear translocation of proCRH within stably transfected CHO-K1 cells and showed that microtubule and actin disrupting agents had no effect upon the nuclear translocation of proCRH. These results, therefore, suggest that nuclear translocation of proCRH is not affected by drugs which disrupt the cytoskeleton and, consequently, modify the diameter of the nuclear pores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318869
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