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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 21 (2005), S. 0 
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: During brain development, GABA and glycine switch from being depolarizing to being hyperpolarizing neurotransmitters. This conversion results from a gradual decrease in the chloride electrochemical equilibrium potential (ECl) of developing neurons, which correlates to an increase in the expression or activity of the potassium chloride cotransporter, KCC2. However, evidence as to whether KCC2 expression is sufficient, in and of itself, to induce this switch is lacking. In order to address this question, we used a gain-of-function approach by over-expressing human KCC2 (hKCC2) in immature cortical neurons, before endogenous up-regulation of KCC2. We found that premature expression of hKCC2 produced a substantial negative shift in the GABA reversal potential and decreased or abolished GABA-elicited calcium responses in cultured neurons. We conclude that KCC2 expression is not only necessary but is also sufficient for ending the depolarizing period of GABA in developing cortical neurons.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A growth trial was conducted to feed juvenile tilapia (initial weight, 9.1±0.1 g), Oreochromis niloticus×O. aureus, isonitrogenous diets for 8 weeks. Six diets were formulated containing 29% crude protein from casein and gelatin, 10% crude fat from soybean oil and refined soybean lecithin and varying levels of corn starch ranging from 6% to 46% at increments of 8%, with corresponding energy to protein (E/P) ratios of 35.6, 37.9, 40.2, 42.5, 44.8 and 47.1 kJ g−1. Weight gain (WG), specific growth rate, feed efficiency ratio and protein efficiency ratio were significantly higher in fish fed diets with starch ≥22% (or E/P ratio ≥40.2 kJ g−1) than in fish fed diets with 6% or 14% starch (or E/P ratio of 35.6 or 37.9 kJ g−1). No further improvement was measured when dietary starch content increased beyond 22%. Body protein retention showed the same general pattern as WG, and was highest in fish fed the 22% starch diet. Body composition was significantly affected by dietary starch level. Fish fed diets with starch ≥30% had significantly higher lipid content than fish fed diets with 6% or 14% starch. Ash content was negatively correlated with starch inclusion level, but moisture and protein contents did not show discernible trends among treatments. Results indicate that hybrid tilapia can utilize 46% dietary starch without growth retardation, while 22% starch in feed for juvenile tilapia containing 29% protein and 10% lipid, or an E/P ratio of 37.9 kJ g−1 is optimal.
    Type of Medium: Electronic Resource
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